Haplotype-resolved de novo assembly of the Vero cell line genome

Abstract The Vero cell line is the most used continuous cell line for viral vaccine manufacturing with more than 40 years of accumulated experience in the vaccine industry. Additionally, the Vero cell line has shown a high affinity for infection by MERS-CoV, SARS-CoV, and recently SARS-CoV-2, emergi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marie-Angélique Sène, Sascha Kiesslich, Haig Djambazian, Jiannis Ragoussis, Yu Xia, Amine A. Kamen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/7132a21470654cf7a9cefa5d2162ab40
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7132a21470654cf7a9cefa5d2162ab40
record_format	dspace
spelling	oai:doaj.org-article:7132a21470654cf7a9cefa5d2162ab402021-12-02T16:46:32ZHaplotype-resolved de novo assembly of the Vero cell line genome10.1038/s41541-021-00358-92059-0105https://doaj.org/article/7132a21470654cf7a9cefa5d2162ab402021-08-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00358-9https://doaj.org/toc/2059-0105Abstract The Vero cell line is the most used continuous cell line for viral vaccine manufacturing with more than 40 years of accumulated experience in the vaccine industry. Additionally, the Vero cell line has shown a high affinity for infection by MERS-CoV, SARS-CoV, and recently SARS-CoV-2, emerging as an important discovery and screening tool to support the global research and development efforts in this COVID-19 pandemic. However, the lack of a reference genome for the Vero cell line has limited our understanding of host–virus interactions underlying such affinity of the Vero cell towards key emerging pathogens, and more importantly our ability to redesign high-yield vaccine production processes using Vero genome editing. In this paper, we present an annotated highly contiguous 2.9 Gb assembly of the Vero cell genome. In addition, several viral genome insertions, including Adeno-associated virus serotypes 3, 4, 7, and 8, have been identified, giving valuable insights into quality control considerations for cell-based vaccine production systems. Variant calling revealed that, in addition to interferon, chemokines, and caspases-related genes lost their functions. Surprisingly, the ACE2 gene, which was previously identified as the host cell entry receptor for SARS-CoV and SARS-CoV-2, also lost function in the Vero genome due to structural variations.Marie-Angélique SèneSascha KiesslichHaig DjambazianJiannis RagoussisYu XiaAmine A. KamenNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Marie-Angélique Sène Sascha Kiesslich Haig Djambazian Jiannis Ragoussis Yu Xia Amine A. Kamen Haplotype-resolved de novo assembly of the Vero cell line genome
description	Abstract The Vero cell line is the most used continuous cell line for viral vaccine manufacturing with more than 40 years of accumulated experience in the vaccine industry. Additionally, the Vero cell line has shown a high affinity for infection by MERS-CoV, SARS-CoV, and recently SARS-CoV-2, emerging as an important discovery and screening tool to support the global research and development efforts in this COVID-19 pandemic. However, the lack of a reference genome for the Vero cell line has limited our understanding of host–virus interactions underlying such affinity of the Vero cell towards key emerging pathogens, and more importantly our ability to redesign high-yield vaccine production processes using Vero genome editing. In this paper, we present an annotated highly contiguous 2.9 Gb assembly of the Vero cell genome. In addition, several viral genome insertions, including Adeno-associated virus serotypes 3, 4, 7, and 8, have been identified, giving valuable insights into quality control considerations for cell-based vaccine production systems. Variant calling revealed that, in addition to interferon, chemokines, and caspases-related genes lost their functions. Surprisingly, the ACE2 gene, which was previously identified as the host cell entry receptor for SARS-CoV and SARS-CoV-2, also lost function in the Vero genome due to structural variations.
format	article
author	Marie-Angélique Sène Sascha Kiesslich Haig Djambazian Jiannis Ragoussis Yu Xia Amine A. Kamen
author_facet	Marie-Angélique Sène Sascha Kiesslich Haig Djambazian Jiannis Ragoussis Yu Xia Amine A. Kamen
author_sort	Marie-Angélique Sène
title	Haplotype-resolved de novo assembly of the Vero cell line genome
title_short	Haplotype-resolved de novo assembly of the Vero cell line genome
title_full	Haplotype-resolved de novo assembly of the Vero cell line genome
title_fullStr	Haplotype-resolved de novo assembly of the Vero cell line genome
title_full_unstemmed	Haplotype-resolved de novo assembly of the Vero cell line genome
title_sort	haplotype-resolved de novo assembly of the vero cell line genome
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7132a21470654cf7a9cefa5d2162ab40
work_keys_str_mv	AT marieangeliquesene haplotyperesolveddenovoassemblyoftheverocelllinegenome AT saschakiesslich haplotyperesolveddenovoassemblyoftheverocelllinegenome AT haigdjambazian haplotyperesolveddenovoassemblyoftheverocelllinegenome AT jiannisragoussis haplotyperesolveddenovoassemblyoftheverocelllinegenome AT yuxia haplotyperesolveddenovoassemblyoftheverocelllinegenome AT amineakamen haplotyperesolveddenovoassemblyoftheverocelllinegenome
_version_	1718383402094166016

Haplotype-resolved de novo assembly of the Vero cell line genome

Ejemplares similares