The effect of magnetic nanoparticles of Fe3O4 on immune function in normal ICR mice

Bao-An Chen1, Nan Jin1, Jun Wang1, Jiahua Ding1, Chong Gao1, Jian Cheng1, Guohua Xia1, Feng Gao1, Yin Zhou1, Yue Chen1, Guina Zhou1, Xiaomao Li2, Yu Zhang3, Men Tang3, Xuemei Wang31Department of Hematology, Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, People&rsqu...

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Autores principales:	Bao-An Chen, Nan Jin, Jun Wang, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/71371b2b35af49d398f116194eaa2145
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spelling	oai:doaj.org-article:71371b2b35af49d398f116194eaa21452021-12-02T04:46:23ZThe effect of magnetic nanoparticles of Fe3O4 on immune function in normal ICR mice1176-91141178-2013https://doaj.org/article/71371b2b35af49d398f116194eaa21452010-08-01T00:00:00Zhttp://www.dovepress.com/the-effect-of-magnetic-nanoparticles-of-fe3o4-on-immune-function-in-no-a5011https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Bao-An Chen1, Nan Jin1, Jun Wang1, Jiahua Ding1, Chong Gao1, Jian Cheng1, Guohua Xia1, Feng Gao1, Yin Zhou1, Yue Chen1, Guina Zhou1, Xiaomao Li2, Yu Zhang3, Men Tang3, Xuemei Wang31Department of Hematology, Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, People&rsquo;s Republic of China; 2Department of Physics, University of Saarland, D-266041 Saarbruechen, Germany; 3National Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing, People&rsquo;s Republic of ChinaAbstract: We investigated the effect of magnetic nanoparticles of Fe3O4 (Fe3O4-MNPs) on the mice immune system. Imprinting control region (ICR) mice were assigned randomly into four groups and treated with normal saline or low, medium, or high doses of Fe3O4-MNPs, respectively. After intravenous administration of Fe3O4-MNPs for 72 hours, the peripheral T cells and the induction of primary immune responses in mice were investigated by flow cytometry and determined using enzyme-linked immunosorbent assay, respectively. The results showed that the ratio of spleen to body weight was not different between the experimental groups and control group (P &gt; 0.05). The lymphocyte transformation rates in the suspension of spleen were higher in low-dose group than those in the control group (P &lt; 0.05), while the proliferation of splenocytes was low in the medium and high groups when compared to the control group (P &lt; 0.05). In peripheral blood, both the proportions of subset CD4+ and CD8+ T lymphocytes in the low-dose group were higher than those in the control group, whereas there was no difference in the number of CD4+ T cells between the medium- and low-dose groups. Interestingly, the Fe3O4-MNPs enhanced the production of interleukin-2 (IL-2), interferon-&gamma;, and IL-10 but did not affect the production of IL-4 in peripheral blood. It is concluded that Fe3O4-MNPs could influence immune functions of normal ICR mice in a dose-dependent manner.Keywords: magnetic nanoparticle of Fe3O4, immune function, splenocyte proliferation, cytokine Bao-An ChenNan JinJun Wanget alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 593-599 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Bao-An Chen Nan Jin Jun Wang et al The effect of magnetic nanoparticles of Fe3O4 on immune function in normal ICR mice
description	Bao-An Chen1, Nan Jin1, Jun Wang1, Jiahua Ding1, Chong Gao1, Jian Cheng1, Guohua Xia1, Feng Gao1, Yin Zhou1, Yue Chen1, Guina Zhou1, Xiaomao Li2, Yu Zhang3, Men Tang3, Xuemei Wang31Department of Hematology, Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, People&rsquo;s Republic of China; 2Department of Physics, University of Saarland, D-266041 Saarbruechen, Germany; 3National Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing, People&rsquo;s Republic of ChinaAbstract: We investigated the effect of magnetic nanoparticles of Fe3O4 (Fe3O4-MNPs) on the mice immune system. Imprinting control region (ICR) mice were assigned randomly into four groups and treated with normal saline or low, medium, or high doses of Fe3O4-MNPs, respectively. After intravenous administration of Fe3O4-MNPs for 72 hours, the peripheral T cells and the induction of primary immune responses in mice were investigated by flow cytometry and determined using enzyme-linked immunosorbent assay, respectively. The results showed that the ratio of spleen to body weight was not different between the experimental groups and control group (P &gt; 0.05). The lymphocyte transformation rates in the suspension of spleen were higher in low-dose group than those in the control group (P &lt; 0.05), while the proliferation of splenocytes was low in the medium and high groups when compared to the control group (P &lt; 0.05). In peripheral blood, both the proportions of subset CD4+ and CD8+ T lymphocytes in the low-dose group were higher than those in the control group, whereas there was no difference in the number of CD4+ T cells between the medium- and low-dose groups. Interestingly, the Fe3O4-MNPs enhanced the production of interleukin-2 (IL-2), interferon-&gamma;, and IL-10 but did not affect the production of IL-4 in peripheral blood. It is concluded that Fe3O4-MNPs could influence immune functions of normal ICR mice in a dose-dependent manner.Keywords: magnetic nanoparticle of Fe3O4, immune function, splenocyte proliferation, cytokine
format	article
author	Bao-An Chen Nan Jin Jun Wang et al
author_facet	Bao-An Chen Nan Jin Jun Wang et al
author_sort	Bao-An Chen
title	The effect of magnetic nanoparticles of Fe3O4 on immune function in normal ICR mice
title_short	The effect of magnetic nanoparticles of Fe3O4 on immune function in normal ICR mice
title_full	The effect of magnetic nanoparticles of Fe3O4 on immune function in normal ICR mice
title_fullStr	The effect of magnetic nanoparticles of Fe3O4 on immune function in normal ICR mice
title_full_unstemmed	The effect of magnetic nanoparticles of Fe3O4 on immune function in normal ICR mice
title_sort	effect of magnetic nanoparticles of fe3o4 on immune function in normal icr mice
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/71371b2b35af49d398f116194eaa2145
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The effect of magnetic nanoparticles of Fe3O4 on immune function in normal ICR mice

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