Next-Generation Sequencing reveals relationship between the larval microbiome and food substrate in the polyphagous Queensland fruit fly

Abstract Insects typically host substantial microbial communities (the ‘microbiome’) that can serve as a vital source of nutrients and also acts as a modulator of immune function. While recent studies have shown that diet is an important influence on the gut microbiome, very little is known about th...

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Autores principales:	Rajib Majumder, Brodie Sutcliffe, Phillip W. Taylor, Toni A. Chapman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/713c1078052e483fb238735902e1149c
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spelling	oai:doaj.org-article:713c1078052e483fb238735902e1149c2021-12-02T15:08:30ZNext-Generation Sequencing reveals relationship between the larval microbiome and food substrate in the polyphagous Queensland fruit fly10.1038/s41598-019-50602-52045-2322https://doaj.org/article/713c1078052e483fb238735902e1149c2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-50602-5https://doaj.org/toc/2045-2322Abstract Insects typically host substantial microbial communities (the ‘microbiome’) that can serve as a vital source of nutrients and also acts as a modulator of immune function. While recent studies have shown that diet is an important influence on the gut microbiome, very little is known about the dynamics underpinning microbial acquisition from natural food sources. Here, we addressed this gap by comparing the microbiome of larvae of the polyphagous fruit fly Bactrocera tryoni (‘Queensland fruit fly’) that were collected from five different fruit types (sapodilla [from two different localities], hog plum, pomegranate, green apple, and quince) from North-east to South-east Australia. Using Next-Generation Sequencing on the Illumina MiSeq platform, we addressed two questions: (1) what bacterial communities are available to B. tryoni larvae from different host fruit; and (2) how does the microbiome vary between B. tryoni larvae and its host fruit? The abundant bacterial taxa were similar for B. tryoni larvae from different fruit despite significant differences in the overall microbial community compositions. Our study suggests that the bacterial community structure of B. tryoni larvae is related less to the host fruit (diet) microbiome and more to vertical transfer of the microbiome during egg laying. Our findings also suggest that geographic location may play a quite limited role in structuring of larval microbiomes. This is the first study to use Next-Generation Sequencing to analyze the microbiome of B. tryoni larvae together with the host fruit, an approach that has enabled greatly increased resolution of relationships between the insect’s microbiome and that of the surrounding host tissues.Rajib MajumderBrodie SutcliffePhillip W. TaylorToni A. ChapmanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rajib Majumder Brodie Sutcliffe Phillip W. Taylor Toni A. Chapman Next-Generation Sequencing reveals relationship between the larval microbiome and food substrate in the polyphagous Queensland fruit fly
description	Abstract Insects typically host substantial microbial communities (the ‘microbiome’) that can serve as a vital source of nutrients and also acts as a modulator of immune function. While recent studies have shown that diet is an important influence on the gut microbiome, very little is known about the dynamics underpinning microbial acquisition from natural food sources. Here, we addressed this gap by comparing the microbiome of larvae of the polyphagous fruit fly Bactrocera tryoni (‘Queensland fruit fly’) that were collected from five different fruit types (sapodilla [from two different localities], hog plum, pomegranate, green apple, and quince) from North-east to South-east Australia. Using Next-Generation Sequencing on the Illumina MiSeq platform, we addressed two questions: (1) what bacterial communities are available to B. tryoni larvae from different host fruit; and (2) how does the microbiome vary between B. tryoni larvae and its host fruit? The abundant bacterial taxa were similar for B. tryoni larvae from different fruit despite significant differences in the overall microbial community compositions. Our study suggests that the bacterial community structure of B. tryoni larvae is related less to the host fruit (diet) microbiome and more to vertical transfer of the microbiome during egg laying. Our findings also suggest that geographic location may play a quite limited role in structuring of larval microbiomes. This is the first study to use Next-Generation Sequencing to analyze the microbiome of B. tryoni larvae together with the host fruit, an approach that has enabled greatly increased resolution of relationships between the insect’s microbiome and that of the surrounding host tissues.
format	article
author	Rajib Majumder Brodie Sutcliffe Phillip W. Taylor Toni A. Chapman
author_facet	Rajib Majumder Brodie Sutcliffe Phillip W. Taylor Toni A. Chapman
author_sort	Rajib Majumder
title	Next-Generation Sequencing reveals relationship between the larval microbiome and food substrate in the polyphagous Queensland fruit fly
title_short	Next-Generation Sequencing reveals relationship between the larval microbiome and food substrate in the polyphagous Queensland fruit fly
title_full	Next-Generation Sequencing reveals relationship between the larval microbiome and food substrate in the polyphagous Queensland fruit fly
title_fullStr	Next-Generation Sequencing reveals relationship between the larval microbiome and food substrate in the polyphagous Queensland fruit fly
title_full_unstemmed	Next-Generation Sequencing reveals relationship between the larval microbiome and food substrate in the polyphagous Queensland fruit fly
title_sort	next-generation sequencing reveals relationship between the larval microbiome and food substrate in the polyphagous queensland fruit fly
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/713c1078052e483fb238735902e1149c
work_keys_str_mv	AT rajibmajumder nextgenerationsequencingrevealsrelationshipbetweenthelarvalmicrobiomeandfoodsubstrateinthepolyphagousqueenslandfruitfly AT brodiesutcliffe nextgenerationsequencingrevealsrelationshipbetweenthelarvalmicrobiomeandfoodsubstrateinthepolyphagousqueenslandfruitfly AT phillipwtaylor nextgenerationsequencingrevealsrelationshipbetweenthelarvalmicrobiomeandfoodsubstrateinthepolyphagousqueenslandfruitfly AT toniachapman nextgenerationsequencingrevealsrelationshipbetweenthelarvalmicrobiomeandfoodsubstrateinthepolyphagousqueenslandfruitfly
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Next-Generation Sequencing reveals relationship between the larval microbiome and food substrate in the polyphagous Queensland fruit fly

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