A Standardized <i>Lindera obtusiloba</i> Extract Improves Endothelial Dysfunction and Attenuates Plaque Development in Hyperlipidemic ApoE-Knockout Mice

<i>Lindera obtusiloba</i> extract (LOE), a traditional herbal medicine used to enhance blood circulation and to reduce inflammation, induced NO-mediated endothelium-dependent relaxation, and reduced the formation of reactive oxygen species (ROS). The study investigated whether LOE improv...

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Autores principales:	Sang-Hyun Ihm, Sin-Hee Park, Jung-Ok Lee, Ok-Ran Kim, Eun-Hye Park, Kyoung-Rak Kim, Jong-Hoon Kim, Byung-Hee Hwang, Ho-Joong Youn, Min-Ho Oak, Kiyuk Chang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Lindera obtusiloba</i> apolipoprotein E-deficient mice atherosclerosis endothelium Botany QK1-989
Acceso en línea:	https://doaj.org/article/713ec3fd3cc548bdb4f12df18a5e6506
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spelling	oai:doaj.org-article:713ec3fd3cc548bdb4f12df18a5e65062021-11-25T18:47:03ZA Standardized <i>Lindera obtusiloba</i> Extract Improves Endothelial Dysfunction and Attenuates Plaque Development in Hyperlipidemic ApoE-Knockout Mice10.3390/plants101124932223-7747https://doaj.org/article/713ec3fd3cc548bdb4f12df18a5e65062021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2493https://doaj.org/toc/2223-7747<i>Lindera obtusiloba</i> extract (LOE), a traditional herbal medicine used to enhance blood circulation and to reduce inflammation, induced NO-mediated endothelium-dependent relaxation, and reduced the formation of reactive oxygen species (ROS). The study investigated whether LOE improves endothelial dysfunction and reduces plaque inflammation and progression by inhibiting ROS generation in a mouse model of atherosclerosis. Eight-week-old apolipoprotein E-deficient (apoE<sup>−/−</sup>) mice fed with a western diet (WD) were randomized into different groups by administering vehicle (0.5% carboxymethylcellulose (CMC)), LOE (100 mg/kg/day), or losartan (30 mg/kg/day) by gavage until the age of 28 weeks. Fourteen male C57BL/6 mice that were fed normal chow and treated with CMC were used as negative controls. Similar to losartan treatment, LOE treatment induced the concentration-dependent relaxation of aorta rings in WD-fed apoE<sup>−/−</sup> mice. LOE treatment significantly reduced the vascular ROS formation and expression of NADPH oxidase subunits, including p22phox and p47phox. Compared with WD-fed apoE<sup>−/−</sup> mice, mice exposed to chronic LOE treatment exhibited reductions in plaque inflammation-related fluorescence signals and atherosclerotic lesions. These effects were greater than those of losartan treatment. In conclusion, LOE treatment improves endothelial dysfunction and reduces plaque inflammation as well as lesion areas by reducing vascular NADPH oxidase-induced ROS generation in a mouse model of atherosclerosis.Sang-Hyun IhmSin-Hee ParkJung-Ok LeeOk-Ran KimEun-Hye ParkKyoung-Rak KimJong-Hoon KimByung-Hee HwangHo-Joong YounMin-Ho OakKiyuk ChangMDPI AGarticle<i>Lindera obtusiloba</i>apolipoprotein E-deficient miceatherosclerosisendotheliumBotanyQK1-989ENPlants, Vol 10, Iss 2493, p 2493 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Lindera obtusiloba</i> apolipoprotein E-deficient mice atherosclerosis endothelium Botany QK1-989
spellingShingle	<i>Lindera obtusiloba</i> apolipoprotein E-deficient mice atherosclerosis endothelium Botany QK1-989 Sang-Hyun Ihm Sin-Hee Park Jung-Ok Lee Ok-Ran Kim Eun-Hye Park Kyoung-Rak Kim Jong-Hoon Kim Byung-Hee Hwang Ho-Joong Youn Min-Ho Oak Kiyuk Chang A Standardized <i>Lindera obtusiloba</i> Extract Improves Endothelial Dysfunction and Attenuates Plaque Development in Hyperlipidemic ApoE-Knockout Mice
description	<i>Lindera obtusiloba</i> extract (LOE), a traditional herbal medicine used to enhance blood circulation and to reduce inflammation, induced NO-mediated endothelium-dependent relaxation, and reduced the formation of reactive oxygen species (ROS). The study investigated whether LOE improves endothelial dysfunction and reduces plaque inflammation and progression by inhibiting ROS generation in a mouse model of atherosclerosis. Eight-week-old apolipoprotein E-deficient (apoE<sup>−/−</sup>) mice fed with a western diet (WD) were randomized into different groups by administering vehicle (0.5% carboxymethylcellulose (CMC)), LOE (100 mg/kg/day), or losartan (30 mg/kg/day) by gavage until the age of 28 weeks. Fourteen male C57BL/6 mice that were fed normal chow and treated with CMC were used as negative controls. Similar to losartan treatment, LOE treatment induced the concentration-dependent relaxation of aorta rings in WD-fed apoE<sup>−/−</sup> mice. LOE treatment significantly reduced the vascular ROS formation and expression of NADPH oxidase subunits, including p22phox and p47phox. Compared with WD-fed apoE<sup>−/−</sup> mice, mice exposed to chronic LOE treatment exhibited reductions in plaque inflammation-related fluorescence signals and atherosclerotic lesions. These effects were greater than those of losartan treatment. In conclusion, LOE treatment improves endothelial dysfunction and reduces plaque inflammation as well as lesion areas by reducing vascular NADPH oxidase-induced ROS generation in a mouse model of atherosclerosis.
format	article
author	Sang-Hyun Ihm Sin-Hee Park Jung-Ok Lee Ok-Ran Kim Eun-Hye Park Kyoung-Rak Kim Jong-Hoon Kim Byung-Hee Hwang Ho-Joong Youn Min-Ho Oak Kiyuk Chang
author_facet	Sang-Hyun Ihm Sin-Hee Park Jung-Ok Lee Ok-Ran Kim Eun-Hye Park Kyoung-Rak Kim Jong-Hoon Kim Byung-Hee Hwang Ho-Joong Youn Min-Ho Oak Kiyuk Chang
author_sort	Sang-Hyun Ihm
title	A Standardized <i>Lindera obtusiloba</i> Extract Improves Endothelial Dysfunction and Attenuates Plaque Development in Hyperlipidemic ApoE-Knockout Mice
title_short	A Standardized <i>Lindera obtusiloba</i> Extract Improves Endothelial Dysfunction and Attenuates Plaque Development in Hyperlipidemic ApoE-Knockout Mice
title_full	A Standardized <i>Lindera obtusiloba</i> Extract Improves Endothelial Dysfunction and Attenuates Plaque Development in Hyperlipidemic ApoE-Knockout Mice
title_fullStr	A Standardized <i>Lindera obtusiloba</i> Extract Improves Endothelial Dysfunction and Attenuates Plaque Development in Hyperlipidemic ApoE-Knockout Mice
title_full_unstemmed	A Standardized <i>Lindera obtusiloba</i> Extract Improves Endothelial Dysfunction and Attenuates Plaque Development in Hyperlipidemic ApoE-Knockout Mice
title_sort	standardized <i>lindera obtusiloba</i> extract improves endothelial dysfunction and attenuates plaque development in hyperlipidemic apoe-knockout mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/713ec3fd3cc548bdb4f12df18a5e6506
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A Standardized <i>Lindera obtusiloba</i> Extract Improves Endothelial Dysfunction and Attenuates Plaque Development in Hyperlipidemic ApoE-Knockout Mice

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