Fusarium graminearum DICER-like-dependent sRNAs are required for the suppression of host immune genes and full virulence.

In filamentous fungi, gene silencing by RNA interference (RNAi) shapes many biological processes, including pathogenicity. Recently, fungal small RNAs (sRNAs) have been shown to act as effectors that disrupt gene activity in interacting plant hosts, thereby undermining their defence responses. We sh...

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Autores principales:	Bernhard Timo Werner, Aline Koch, Ena Šečić, Jonas Engelhardt, Lukas Jelonek, Jens Steinbrenner, Karl-Heinz Kogel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7141f93ceb054042b345db6f46277e99
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spelling	oai:doaj.org-article:7141f93ceb054042b345db6f46277e992021-12-02T20:18:42ZFusarium graminearum DICER-like-dependent sRNAs are required for the suppression of host immune genes and full virulence.1932-620310.1371/journal.pone.0252365https://doaj.org/article/7141f93ceb054042b345db6f46277e992021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252365https://doaj.org/toc/1932-6203In filamentous fungi, gene silencing by RNA interference (RNAi) shapes many biological processes, including pathogenicity. Recently, fungal small RNAs (sRNAs) have been shown to act as effectors that disrupt gene activity in interacting plant hosts, thereby undermining their defence responses. We show here that the devastating mycotoxin-producing ascomycete Fusarium graminearum (Fg) utilizes DICER-like (DCL)-dependent sRNAs to target defence genes in two Poaceae hosts, barley (Hordeum vulgare, Hv) and Brachypodium distachyon (Bd). We identified 104 Fg-sRNAs with sequence homology to host genes that were repressed during interactions of Fg and Hv, while they accumulated in plants infected by the DCL double knock-out (dKO) mutant PH1-dcl1/2. The strength of target gene expression correlated with the abundance of the corresponding Fg-sRNA. Specifically, the abundance of three tRNA-derived fragments (tRFs) targeting immunity-related Ethylene overproducer 1-like 1 (HvEOL1) and three Poaceae orthologues of Arabidopsis thaliana BRI1-associated receptor kinase 1 (HvBAK1, HvSERK2 and BdSERK2) was dependent on fungal DCL. Additionally, RNA-ligase-mediated Rapid Amplification of cDNA Ends (RLM-RACE) identified infection-specific degradation products for the three barley gene transcripts, consistent with the possibility that tRFs contribute to fungal virulence via targeted gene silencing.Bernhard Timo WernerAline KochEna ŠečićJonas EngelhardtLukas JelonekJens SteinbrennerKarl-Heinz KogelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0252365 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bernhard Timo Werner Aline Koch Ena Šečić Jonas Engelhardt Lukas Jelonek Jens Steinbrenner Karl-Heinz Kogel Fusarium graminearum DICER-like-dependent sRNAs are required for the suppression of host immune genes and full virulence.
description	In filamentous fungi, gene silencing by RNA interference (RNAi) shapes many biological processes, including pathogenicity. Recently, fungal small RNAs (sRNAs) have been shown to act as effectors that disrupt gene activity in interacting plant hosts, thereby undermining their defence responses. We show here that the devastating mycotoxin-producing ascomycete Fusarium graminearum (Fg) utilizes DICER-like (DCL)-dependent sRNAs to target defence genes in two Poaceae hosts, barley (Hordeum vulgare, Hv) and Brachypodium distachyon (Bd). We identified 104 Fg-sRNAs with sequence homology to host genes that were repressed during interactions of Fg and Hv, while they accumulated in plants infected by the DCL double knock-out (dKO) mutant PH1-dcl1/2. The strength of target gene expression correlated with the abundance of the corresponding Fg-sRNA. Specifically, the abundance of three tRNA-derived fragments (tRFs) targeting immunity-related Ethylene overproducer 1-like 1 (HvEOL1) and three Poaceae orthologues of Arabidopsis thaliana BRI1-associated receptor kinase 1 (HvBAK1, HvSERK2 and BdSERK2) was dependent on fungal DCL. Additionally, RNA-ligase-mediated Rapid Amplification of cDNA Ends (RLM-RACE) identified infection-specific degradation products for the three barley gene transcripts, consistent with the possibility that tRFs contribute to fungal virulence via targeted gene silencing.
format	article
author	Bernhard Timo Werner Aline Koch Ena Šečić Jonas Engelhardt Lukas Jelonek Jens Steinbrenner Karl-Heinz Kogel
author_facet	Bernhard Timo Werner Aline Koch Ena Šečić Jonas Engelhardt Lukas Jelonek Jens Steinbrenner Karl-Heinz Kogel
author_sort	Bernhard Timo Werner
title	Fusarium graminearum DICER-like-dependent sRNAs are required for the suppression of host immune genes and full virulence.
title_short	Fusarium graminearum DICER-like-dependent sRNAs are required for the suppression of host immune genes and full virulence.
title_full	Fusarium graminearum DICER-like-dependent sRNAs are required for the suppression of host immune genes and full virulence.
title_fullStr	Fusarium graminearum DICER-like-dependent sRNAs are required for the suppression of host immune genes and full virulence.
title_full_unstemmed	Fusarium graminearum DICER-like-dependent sRNAs are required for the suppression of host immune genes and full virulence.
title_sort	fusarium graminearum dicer-like-dependent srnas are required for the suppression of host immune genes and full virulence.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/7141f93ceb054042b345db6f46277e99
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Fusarium graminearum DICER-like-dependent sRNAs are required for the suppression of host immune genes and full virulence.

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