Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle

Abstract The mechanistic target of rapamycin (mTOR) is a central mediator of protein synthesis in skeletal muscle. We utilized immunofluorescence approaches to study mTOR cellular distribution and protein-protein co-localisation in human skeletal muscle in the basal state as well as immediately, 1 a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhe Song, Daniel R. Moore, Nathan Hodson, Carl Ward, Jessica R. Dent, Mary F. O’Leary, Andrew M. Shaw, D. Lee Hamilton, Sovan Sarkar, Yann-Gaël Gangloff, Troy A. Hornberger, Lawrence L. Spriet, George J. Heigenhauser, Andrew Philp
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/71453322f31c4042b79fba8f35f34ad7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:71453322f31c4042b79fba8f35f34ad7
record_format	dspace
spelling	oai:doaj.org-article:71453322f31c4042b79fba8f35f34ad72021-12-02T16:06:51ZResistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle10.1038/s41598-017-05483-x2045-2322https://doaj.org/article/71453322f31c4042b79fba8f35f34ad72017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05483-xhttps://doaj.org/toc/2045-2322Abstract The mechanistic target of rapamycin (mTOR) is a central mediator of protein synthesis in skeletal muscle. We utilized immunofluorescence approaches to study mTOR cellular distribution and protein-protein co-localisation in human skeletal muscle in the basal state as well as immediately, 1 and 3 h after an acute bout of resistance exercise in a fed (FED; 20 g Protein/40 g carbohydrate/1 g fat) or energy-free control (CON) state. mTOR and the lysosomal protein LAMP2 were highly co-localised in basal samples. Resistance exercise resulted in rapid translocation of mTOR/LAMP2 towards the cell membrane. Concurrently, resistance exercise led to the dissociation of TSC2 from Rheb and increased in the co-localisation of mTOR and Rheb post exercise in both FED and CON. In addition, mTOR co-localised with Eukaryotic translation initiation factor 3 subunit F (eIF3F) at the cell membrane post-exercise in both groups, with the response significantly greater at 1 h of recovery in the FED compared to CON. Collectively our data demonstrate that cellular trafficking of mTOR occurs in human muscle in response to an anabolic stimulus, events that appear to be primarily influenced by muscle contraction. The translocation and association of mTOR with positive regulators (i.e. Rheb and eIF3F) is consistent with an enhanced mRNA translational capacity after resistance exercise.Zhe SongDaniel R. MooreNathan HodsonCarl WardJessica R. DentMary F. O’LearyAndrew M. ShawD. Lee HamiltonSovan SarkarYann-Gaël GangloffTroy A. HornbergerLawrence L. SprietGeorge J. HeigenhauserAndrew PhilpNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhe Song Daniel R. Moore Nathan Hodson Carl Ward Jessica R. Dent Mary F. O’Leary Andrew M. Shaw D. Lee Hamilton Sovan Sarkar Yann-Gaël Gangloff Troy A. Hornberger Lawrence L. Spriet George J. Heigenhauser Andrew Philp Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle
description	Abstract The mechanistic target of rapamycin (mTOR) is a central mediator of protein synthesis in skeletal muscle. We utilized immunofluorescence approaches to study mTOR cellular distribution and protein-protein co-localisation in human skeletal muscle in the basal state as well as immediately, 1 and 3 h after an acute bout of resistance exercise in a fed (FED; 20 g Protein/40 g carbohydrate/1 g fat) or energy-free control (CON) state. mTOR and the lysosomal protein LAMP2 were highly co-localised in basal samples. Resistance exercise resulted in rapid translocation of mTOR/LAMP2 towards the cell membrane. Concurrently, resistance exercise led to the dissociation of TSC2 from Rheb and increased in the co-localisation of mTOR and Rheb post exercise in both FED and CON. In addition, mTOR co-localised with Eukaryotic translation initiation factor 3 subunit F (eIF3F) at the cell membrane post-exercise in both groups, with the response significantly greater at 1 h of recovery in the FED compared to CON. Collectively our data demonstrate that cellular trafficking of mTOR occurs in human muscle in response to an anabolic stimulus, events that appear to be primarily influenced by muscle contraction. The translocation and association of mTOR with positive regulators (i.e. Rheb and eIF3F) is consistent with an enhanced mRNA translational capacity after resistance exercise.
format	article
author	Zhe Song Daniel R. Moore Nathan Hodson Carl Ward Jessica R. Dent Mary F. O’Leary Andrew M. Shaw D. Lee Hamilton Sovan Sarkar Yann-Gaël Gangloff Troy A. Hornberger Lawrence L. Spriet George J. Heigenhauser Andrew Philp
author_facet	Zhe Song Daniel R. Moore Nathan Hodson Carl Ward Jessica R. Dent Mary F. O’Leary Andrew M. Shaw D. Lee Hamilton Sovan Sarkar Yann-Gaël Gangloff Troy A. Hornberger Lawrence L. Spriet George J. Heigenhauser Andrew Philp
author_sort	Zhe Song
title	Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle
title_short	Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle
title_full	Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle
title_fullStr	Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle
title_full_unstemmed	Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle
title_sort	resistance exercise initiates mechanistic target of rapamycin (mtor) translocation and protein complex co-localisation in human skeletal muscle
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/71453322f31c4042b79fba8f35f34ad7
work_keys_str_mv	AT zhesong resistanceexerciseinitiatesmechanistictargetofrapamycinmtortranslocationandproteincomplexcolocalisationinhumanskeletalmuscle AT danielrmoore resistanceexerciseinitiatesmechanistictargetofrapamycinmtortranslocationandproteincomplexcolocalisationinhumanskeletalmuscle AT nathanhodson resistanceexerciseinitiatesmechanistictargetofrapamycinmtortranslocationandproteincomplexcolocalisationinhumanskeletalmuscle AT carlward resistanceexerciseinitiatesmechanistictargetofrapamycinmtortranslocationandproteincomplexcolocalisationinhumanskeletalmuscle AT jessicardent resistanceexerciseinitiatesmechanistictargetofrapamycinmtortranslocationandproteincomplexcolocalisationinhumanskeletalmuscle AT maryfoleary resistanceexerciseinitiatesmechanistictargetofrapamycinmtortranslocationandproteincomplexcolocalisationinhumanskeletalmuscle AT andrewmshaw resistanceexerciseinitiatesmechanistictargetofrapamycinmtortranslocationandproteincomplexcolocalisationinhumanskeletalmuscle AT dleehamilton resistanceexerciseinitiatesmechanistictargetofrapamycinmtortranslocationandproteincomplexcolocalisationinhumanskeletalmuscle AT sovansarkar resistanceexerciseinitiatesmechanistictargetofrapamycinmtortranslocationandproteincomplexcolocalisationinhumanskeletalmuscle AT yanngaelgangloff resistanceexerciseinitiatesmechanistictargetofrapamycinmtortranslocationandproteincomplexcolocalisationinhumanskeletalmuscle AT troyahornberger resistanceexerciseinitiatesmechanistictargetofrapamycinmtortranslocationandproteincomplexcolocalisationinhumanskeletalmuscle AT lawrencelspriet resistanceexerciseinitiatesmechanistictargetofrapamycinmtortranslocationandproteincomplexcolocalisationinhumanskeletalmuscle AT georgejheigenhauser resistanceexerciseinitiatesmechanistictargetofrapamycinmtortranslocationandproteincomplexcolocalisationinhumanskeletalmuscle AT andrewphilp resistanceexerciseinitiatesmechanistictargetofrapamycinmtortranslocationandproteincomplexcolocalisationinhumanskeletalmuscle
_version_	1718384847429304320

Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle

Ejemplares similares