Charge-Modulated Synthesis of Highly Stable Iron Oxide Nanoparticles for In Vitro and In Vivo Toxicity Evaluation

The surface charge of iron oxide nanoparticles (IONPs) plays a critical role in the interactions between nanoparticles and biological components, which significantly affects their toxicity in vitro and in vivo. In this study, we synthesized three differently charged IONPs (negative, neutral, and pos...

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Autores principales:	Sunyoung Woo, Soojin Kim, Hyunhong Kim, Young Woo Cheon, Seokjoo Yoon, Jung-Hwa Oh, Jongnam Park
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	iron oxide nanoparticles toxicity biocompatibility colloidal stability PEG ligands PEG ligands Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/714d038f910042e79ee90b6e6e063b39
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spelling	oai:doaj.org-article:714d038f910042e79ee90b6e6e063b392021-11-25T18:32:12ZCharge-Modulated Synthesis of Highly Stable Iron Oxide Nanoparticles for In Vitro and In Vivo Toxicity Evaluation10.3390/nano111130682079-4991https://doaj.org/article/714d038f910042e79ee90b6e6e063b392021-11-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/3068https://doaj.org/toc/2079-4991The surface charge of iron oxide nanoparticles (IONPs) plays a critical role in the interactions between nanoparticles and biological components, which significantly affects their toxicity in vitro and in vivo. In this study, we synthesized three differently charged IONPs (negative, neutral, and positive) based on catechol-derived dopamine, polyethylene glycol, carboxylic acid, and amine groups, via reversible addition–fragmentation chain transfer-mediated polymerization (RAFT polymerization) and ligand exchange. The zeta potentials of the negative, neutral, and positive IONPs were −39, −0.6, and +32 mV, respectively, and all three IONPs showed long-term colloidal stability for three months in an aqueous solution without agglomeration. The cytotoxicity of the IONPs was studied by analyzing cell viability and morphological alteration in three human cell lines, A549, Huh-7, and SH-SY5Y. Neither IONP caused significant cellular damage in any of the three cell lines. Furthermore, the IONPs showed no acute toxicity in BALB/c mice, in hematological and histological analyses. These results indicate that our charged IONPs, having high colloidal stability and biocompatibility, are viable for bio-applications.Sunyoung WooSoojin KimHyunhong KimYoung Woo CheonSeokjoo YoonJung-Hwa OhJongnam ParkMDPI AGarticleiron oxide nanoparticlestoxicitybiocompatibilitycolloidal stability PEG ligandsPEG ligandsChemistryQD1-999ENNanomaterials, Vol 11, Iss 3068, p 3068 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	iron oxide nanoparticles toxicity biocompatibility colloidal stability PEG ligands PEG ligands Chemistry QD1-999
spellingShingle	iron oxide nanoparticles toxicity biocompatibility colloidal stability PEG ligands PEG ligands Chemistry QD1-999 Sunyoung Woo Soojin Kim Hyunhong Kim Young Woo Cheon Seokjoo Yoon Jung-Hwa Oh Jongnam Park Charge-Modulated Synthesis of Highly Stable Iron Oxide Nanoparticles for In Vitro and In Vivo Toxicity Evaluation
description	The surface charge of iron oxide nanoparticles (IONPs) plays a critical role in the interactions between nanoparticles and biological components, which significantly affects their toxicity in vitro and in vivo. In this study, we synthesized three differently charged IONPs (negative, neutral, and positive) based on catechol-derived dopamine, polyethylene glycol, carboxylic acid, and amine groups, via reversible addition–fragmentation chain transfer-mediated polymerization (RAFT polymerization) and ligand exchange. The zeta potentials of the negative, neutral, and positive IONPs were −39, −0.6, and +32 mV, respectively, and all three IONPs showed long-term colloidal stability for three months in an aqueous solution without agglomeration. The cytotoxicity of the IONPs was studied by analyzing cell viability and morphological alteration in three human cell lines, A549, Huh-7, and SH-SY5Y. Neither IONP caused significant cellular damage in any of the three cell lines. Furthermore, the IONPs showed no acute toxicity in BALB/c mice, in hematological and histological analyses. These results indicate that our charged IONPs, having high colloidal stability and biocompatibility, are viable for bio-applications.
format	article
author	Sunyoung Woo Soojin Kim Hyunhong Kim Young Woo Cheon Seokjoo Yoon Jung-Hwa Oh Jongnam Park
author_facet	Sunyoung Woo Soojin Kim Hyunhong Kim Young Woo Cheon Seokjoo Yoon Jung-Hwa Oh Jongnam Park
author_sort	Sunyoung Woo
title	Charge-Modulated Synthesis of Highly Stable Iron Oxide Nanoparticles for In Vitro and In Vivo Toxicity Evaluation
title_short	Charge-Modulated Synthesis of Highly Stable Iron Oxide Nanoparticles for In Vitro and In Vivo Toxicity Evaluation
title_full	Charge-Modulated Synthesis of Highly Stable Iron Oxide Nanoparticles for In Vitro and In Vivo Toxicity Evaluation
title_fullStr	Charge-Modulated Synthesis of Highly Stable Iron Oxide Nanoparticles for In Vitro and In Vivo Toxicity Evaluation
title_full_unstemmed	Charge-Modulated Synthesis of Highly Stable Iron Oxide Nanoparticles for In Vitro and In Vivo Toxicity Evaluation
title_sort	charge-modulated synthesis of highly stable iron oxide nanoparticles for in vitro and in vivo toxicity evaluation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/714d038f910042e79ee90b6e6e063b39
work_keys_str_mv	AT sunyoungwoo chargemodulatedsynthesisofhighlystableironoxidenanoparticlesforinvitroandinvivotoxicityevaluation AT soojinkim chargemodulatedsynthesisofhighlystableironoxidenanoparticlesforinvitroandinvivotoxicityevaluation AT hyunhongkim chargemodulatedsynthesisofhighlystableironoxidenanoparticlesforinvitroandinvivotoxicityevaluation AT youngwoocheon chargemodulatedsynthesisofhighlystableironoxidenanoparticlesforinvitroandinvivotoxicityevaluation AT seokjooyoon chargemodulatedsynthesisofhighlystableironoxidenanoparticlesforinvitroandinvivotoxicityevaluation AT junghwaoh chargemodulatedsynthesisofhighlystableironoxidenanoparticlesforinvitroandinvivotoxicityevaluation AT jongnampark chargemodulatedsynthesisofhighlystableironoxidenanoparticlesforinvitroandinvivotoxicityevaluation
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Charge-Modulated Synthesis of Highly Stable Iron Oxide Nanoparticles for In Vitro and In Vivo Toxicity Evaluation

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