Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM

2D crystals of the MloK1 potassium channel diffract to only 10 Å. Here the authors present a method, where they apply cryo-EM single-particle image processing to recorded 2D crystal images, which reveals conformational conformational variability of MloK1 within the crystals and enabled them to deter...

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Auteurs principaux:	Ricardo D. Righetto, Nikhil Biyani, Julia Kowal, Mohamed Chami, Henning Stahlberg
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2019
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/714e4f4cdcf04cc487fb9cd2715a0b91
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id	oai:doaj.org-article:714e4f4cdcf04cc487fb9cd2715a0b91
record_format	dspace
spelling	oai:doaj.org-article:714e4f4cdcf04cc487fb9cd2715a0b912021-12-02T15:35:40ZRetrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM10.1038/s41467-019-09661-52041-1723https://doaj.org/article/714e4f4cdcf04cc487fb9cd2715a0b912019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09661-5https://doaj.org/toc/2041-17232D crystals of the MloK1 potassium channel diffract to only 10 Å. Here the authors present a method, where they apply cryo-EM single-particle image processing to recorded 2D crystal images, which reveals conformational conformational variability of MloK1 within the crystals and enabled them to determine the structure at 4 Å resolution.Ricardo D. RighettoNikhil BiyaniJulia KowalMohamed ChamiHenning StahlbergNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Ricardo D. Righetto Nikhil Biyani Julia Kowal Mohamed Chami Henning Stahlberg Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM
description	2D crystals of the MloK1 potassium channel diffract to only 10 Å. Here the authors present a method, where they apply cryo-EM single-particle image processing to recorded 2D crystal images, which reveals conformational conformational variability of MloK1 within the crystals and enabled them to determine the structure at 4 Å resolution.
format	article
author	Ricardo D. Righetto Nikhil Biyani Julia Kowal Mohamed Chami Henning Stahlberg
author_facet	Ricardo D. Righetto Nikhil Biyani Julia Kowal Mohamed Chami Henning Stahlberg
author_sort	Ricardo D. Righetto
title	Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM
title_short	Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM
title_full	Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM
title_fullStr	Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM
title_full_unstemmed	Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM
title_sort	retrieving high-resolution information from disordered 2d crystals by single-particle cryo-em
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/714e4f4cdcf04cc487fb9cd2715a0b91
work_keys_str_mv	AT ricardodrighetto retrievinghighresolutioninformationfromdisordered2dcrystalsbysingleparticlecryoem AT nikhilbiyani retrievinghighresolutioninformationfromdisordered2dcrystalsbysingleparticlecryoem AT juliakowal retrievinghighresolutioninformationfromdisordered2dcrystalsbysingleparticlecryoem AT mohamedchami retrievinghighresolutioninformationfromdisordered2dcrystalsbysingleparticlecryoem AT henningstahlberg retrievinghighresolutioninformationfromdisordered2dcrystalsbysingleparticlecryoem
_version_	1718386480756293632

Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM

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