Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM

2D crystals of the MloK1 potassium channel diffract to only 10 Å. Here the authors present a method, where they apply cryo-EM single-particle image processing to recorded 2D crystal images, which reveals conformational conformational variability of MloK1 within the crystals and enabled them to deter...

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Autores principales: Ricardo D. Righetto, Nikhil Biyani, Julia Kowal, Mohamed Chami, Henning Stahlberg
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Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/714e4f4cdcf04cc487fb9cd2715a0b91
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spelling oai:doaj.org-article:714e4f4cdcf04cc487fb9cd2715a0b912021-12-02T15:35:40ZRetrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM10.1038/s41467-019-09661-52041-1723https://doaj.org/article/714e4f4cdcf04cc487fb9cd2715a0b912019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09661-5https://doaj.org/toc/2041-17232D crystals of the MloK1 potassium channel diffract to only 10 Å. Here the authors present a method, where they apply cryo-EM single-particle image processing to recorded 2D crystal images, which reveals conformational conformational variability of MloK1 within the crystals and enabled them to determine the structure at 4 Å resolution.Ricardo D. RighettoNikhil BiyaniJulia KowalMohamed ChamiHenning StahlbergNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Ricardo D. Righetto
Nikhil Biyani
Julia Kowal
Mohamed Chami
Henning Stahlberg
Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM
description 2D crystals of the MloK1 potassium channel diffract to only 10 Å. Here the authors present a method, where they apply cryo-EM single-particle image processing to recorded 2D crystal images, which reveals conformational conformational variability of MloK1 within the crystals and enabled them to determine the structure at 4 Å resolution.
format article
author Ricardo D. Righetto
Nikhil Biyani
Julia Kowal
Mohamed Chami
Henning Stahlberg
author_facet Ricardo D. Righetto
Nikhil Biyani
Julia Kowal
Mohamed Chami
Henning Stahlberg
author_sort Ricardo D. Righetto
title Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM
title_short Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM
title_full Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM
title_fullStr Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM
title_full_unstemmed Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM
title_sort retrieving high-resolution information from disordered 2d crystals by single-particle cryo-em
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/714e4f4cdcf04cc487fb9cd2715a0b91
work_keys_str_mv AT ricardodrighetto retrievinghighresolutioninformationfromdisordered2dcrystalsbysingleparticlecryoem
AT nikhilbiyani retrievinghighresolutioninformationfromdisordered2dcrystalsbysingleparticlecryoem
AT juliakowal retrievinghighresolutioninformationfromdisordered2dcrystalsbysingleparticlecryoem
AT mohamedchami retrievinghighresolutioninformationfromdisordered2dcrystalsbysingleparticlecryoem
AT henningstahlberg retrievinghighresolutioninformationfromdisordered2dcrystalsbysingleparticlecryoem
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