Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM

2D crystals of the MloK1 potassium channel diffract to only 10 Å. Here the authors present a method, where they apply cryo-EM single-particle image processing to recorded 2D crystal images, which reveals conformational conformational variability of MloK1 within the crystals and enabled them to deter...

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Auteurs principaux: Ricardo D. Righetto, Nikhil Biyani, Julia Kowal, Mohamed Chami, Henning Stahlberg
Format: article
Langue:EN
Publié: Nature Portfolio 2019
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Accès en ligne:https://doaj.org/article/714e4f4cdcf04cc487fb9cd2715a0b91
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