Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination

Abstract Rice lines with slower starch digestibility provide opportunities in mitigating the global rise in type II diabetes and related non-communicable diseases. However, screening for low glycemic index (GI) in rice breeding programs is not possible due to time and cost constraints. This study ev...

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Autores principales:	Maria Krishna de Guzman, Sabiha Parween, Vito M. Butardo, Crisline Mae Alhambra, Roslen Anacleto, Christiane Seiler, Anthony R. Bird, Chung-Ping Chow, Nese Sreenivasulu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7159edb612564c3b80f721bbf9e60f05
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spelling	oai:doaj.org-article:7159edb612564c3b80f721bbf9e60f052021-12-02T16:08:19ZInvestigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination10.1038/s41598-017-06026-02045-2322https://doaj.org/article/7159edb612564c3b80f721bbf9e60f052017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06026-0https://doaj.org/toc/2045-2322Abstract Rice lines with slower starch digestibility provide opportunities in mitigating the global rise in type II diabetes and related non-communicable diseases. However, screening for low glycemic index (GI) in rice breeding programs is not possible due to time and cost constraints. This study evaluated the feasibility of using in vitro cooked grain amylolysis, starch mobilization patterns during seed germination, and variation in starch structure and composition in the mature seed to differentiate patterns of starch digestibility. Mobilization patterns of total starch, resistant starch, amylose and amylopectin chains, and free sugars during seed germination revealed that the process is analogous to digestion in the human gastrointestinal tract. The combination of these biochemical markers can be used as an alternative measure to predict GI. Additionally, transcriptome analysis of stored mRNA transcripts in high and low GI lines detected differences in starch metabolism and confirmed the importance of seed storage pathways in influencing digestibility. Pathway analyses supported by metabolomics data revealed that resistant starch, cell wall non-starch polysaccharides and flavonoids potentially contribute to slower digestibility. These new insights can guide precision breeding programs to produce low GI rice with acceptable cooking quality to help mitigate the burden of diet-associated lifestyle diseases.Maria Krishna de GuzmanSabiha ParweenVito M. ButardoCrisline Mae AlhambraRoslen AnacletoChristiane SeilerAnthony R. BirdChung-Ping ChowNese SreenivasuluNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Krishna de Guzman Sabiha Parween Vito M. Butardo Crisline Mae Alhambra Roslen Anacleto Christiane Seiler Anthony R. Bird Chung-Ping Chow Nese Sreenivasulu Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination
description	Abstract Rice lines with slower starch digestibility provide opportunities in mitigating the global rise in type II diabetes and related non-communicable diseases. However, screening for low glycemic index (GI) in rice breeding programs is not possible due to time and cost constraints. This study evaluated the feasibility of using in vitro cooked grain amylolysis, starch mobilization patterns during seed germination, and variation in starch structure and composition in the mature seed to differentiate patterns of starch digestibility. Mobilization patterns of total starch, resistant starch, amylose and amylopectin chains, and free sugars during seed germination revealed that the process is analogous to digestion in the human gastrointestinal tract. The combination of these biochemical markers can be used as an alternative measure to predict GI. Additionally, transcriptome analysis of stored mRNA transcripts in high and low GI lines detected differences in starch metabolism and confirmed the importance of seed storage pathways in influencing digestibility. Pathway analyses supported by metabolomics data revealed that resistant starch, cell wall non-starch polysaccharides and flavonoids potentially contribute to slower digestibility. These new insights can guide precision breeding programs to produce low GI rice with acceptable cooking quality to help mitigate the burden of diet-associated lifestyle diseases.
format	article
author	Maria Krishna de Guzman Sabiha Parween Vito M. Butardo Crisline Mae Alhambra Roslen Anacleto Christiane Seiler Anthony R. Bird Chung-Ping Chow Nese Sreenivasulu
author_facet	Maria Krishna de Guzman Sabiha Parween Vito M. Butardo Crisline Mae Alhambra Roslen Anacleto Christiane Seiler Anthony R. Bird Chung-Ping Chow Nese Sreenivasulu
author_sort	Maria Krishna de Guzman
title	Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination
title_short	Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination
title_full	Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination
title_fullStr	Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination
title_full_unstemmed	Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination
title_sort	investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7159edb612564c3b80f721bbf9e60f05
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Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination

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