Proteome Analysis of Camellia nitidissima Chi Revealed Its Role in Colon Cancer Through the Apoptosis and Ferroptosis Pathway

Colon cancer is the third most common cancer in the world with a high mortality rate. At present, surgery combined with radiotherapy and chemotherapy is the primary treatment, but patient prognosis remains poor. Traditional Chinese medicine (TCM) has become a complementary and alternative source of...

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Autores principales:	Yiwei Chen, Fan Zhang, Zhengcai Du, Jinling Xie, Lei Xia, Xiaotao Hou, Erwei Hao, Jiagang Deng
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Camellia nitidissima Chi colon cancer ferroptosis proteomics 4D label-free Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/716397954dde46b0b7af505167a72da6
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spelling	oai:doaj.org-article:716397954dde46b0b7af505167a72da62021-11-09T06:04:23ZProteome Analysis of Camellia nitidissima Chi Revealed Its Role in Colon Cancer Through the Apoptosis and Ferroptosis Pathway2234-943X10.3389/fonc.2021.727130https://doaj.org/article/716397954dde46b0b7af505167a72da62021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fonc.2021.727130/fullhttps://doaj.org/toc/2234-943XColon cancer is the third most common cancer in the world with a high mortality rate. At present, surgery combined with radiotherapy and chemotherapy is the primary treatment, but patient prognosis remains poor. Traditional Chinese medicine (TCM) has become a complementary and alternative source of anti-cancer drugs. Camellia nitidissima Chi (CNC) is a TCM used to treat a variety of cancers. However, the role of CNC in cancer remains elusive, and its effect and mechanism on colon cancer have not been reported. Here, we show that CNC exerts an excellent inhibitory effect on colon cancer proliferation and apoptosis induction in vitro and in vivo. We performed label free-based quantitative proteomic analysis to evaluate the HCT116 cells treated with CNC. Our data revealed a total of 363 differentially expressed proteins, of which 157 were up-regulated and 206 down-regulated. Gene Ontology enrichment analysis showed that these proteins were involved in tumor occurrence and development through multiple biological processes such as cell proliferation, cell apoptosis, cell cycle, and cell death. Interestingly, we also found significant changes in ferroptosis pathways. The role of essential proteins glutathione peroxidase 4 (GPX4) and heme oxygenase-1 (HMOX1) were verified. CNC decreased the expression of GPX4 and increased the expression of HMOX1 at the mRNA and protein levels in vivo and in vitro. Collectively, these findings reveal that CNC regulates colon cancer progression via the ferroptosis pathway and could be an attractive treatment for colon cancer.Yiwei ChenYiwei ChenYiwei ChenFan ZhangFan ZhangZhengcai DuZhengcai DuZhengcai DuJinling XieJinling XieLei XiaLei XiaXiaotao HouXiaotao HouXiaotao HouErwei HaoErwei HaoErwei HaoJiagang DengJiagang DengJiagang DengFrontiers Media S.A.articleCamellia nitidissima Chicolon cancerferroptosisproteomics4D label-freeNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENFrontiers in Oncology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Camellia nitidissima Chi colon cancer ferroptosis proteomics 4D label-free Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Camellia nitidissima Chi colon cancer ferroptosis proteomics 4D label-free Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Yiwei Chen Yiwei Chen Yiwei Chen Fan Zhang Fan Zhang Zhengcai Du Zhengcai Du Zhengcai Du Jinling Xie Jinling Xie Lei Xia Lei Xia Xiaotao Hou Xiaotao Hou Xiaotao Hou Erwei Hao Erwei Hao Erwei Hao Jiagang Deng Jiagang Deng Jiagang Deng Proteome Analysis of Camellia nitidissima Chi Revealed Its Role in Colon Cancer Through the Apoptosis and Ferroptosis Pathway
description	Colon cancer is the third most common cancer in the world with a high mortality rate. At present, surgery combined with radiotherapy and chemotherapy is the primary treatment, but patient prognosis remains poor. Traditional Chinese medicine (TCM) has become a complementary and alternative source of anti-cancer drugs. Camellia nitidissima Chi (CNC) is a TCM used to treat a variety of cancers. However, the role of CNC in cancer remains elusive, and its effect and mechanism on colon cancer have not been reported. Here, we show that CNC exerts an excellent inhibitory effect on colon cancer proliferation and apoptosis induction in vitro and in vivo. We performed label free-based quantitative proteomic analysis to evaluate the HCT116 cells treated with CNC. Our data revealed a total of 363 differentially expressed proteins, of which 157 were up-regulated and 206 down-regulated. Gene Ontology enrichment analysis showed that these proteins were involved in tumor occurrence and development through multiple biological processes such as cell proliferation, cell apoptosis, cell cycle, and cell death. Interestingly, we also found significant changes in ferroptosis pathways. The role of essential proteins glutathione peroxidase 4 (GPX4) and heme oxygenase-1 (HMOX1) were verified. CNC decreased the expression of GPX4 and increased the expression of HMOX1 at the mRNA and protein levels in vivo and in vitro. Collectively, these findings reveal that CNC regulates colon cancer progression via the ferroptosis pathway and could be an attractive treatment for colon cancer.
format	article
author	Yiwei Chen Yiwei Chen Yiwei Chen Fan Zhang Fan Zhang Zhengcai Du Zhengcai Du Zhengcai Du Jinling Xie Jinling Xie Lei Xia Lei Xia Xiaotao Hou Xiaotao Hou Xiaotao Hou Erwei Hao Erwei Hao Erwei Hao Jiagang Deng Jiagang Deng Jiagang Deng
author_facet	Yiwei Chen Yiwei Chen Yiwei Chen Fan Zhang Fan Zhang Zhengcai Du Zhengcai Du Zhengcai Du Jinling Xie Jinling Xie Lei Xia Lei Xia Xiaotao Hou Xiaotao Hou Xiaotao Hou Erwei Hao Erwei Hao Erwei Hao Jiagang Deng Jiagang Deng Jiagang Deng
author_sort	Yiwei Chen
title	Proteome Analysis of Camellia nitidissima Chi Revealed Its Role in Colon Cancer Through the Apoptosis and Ferroptosis Pathway
title_short	Proteome Analysis of Camellia nitidissima Chi Revealed Its Role in Colon Cancer Through the Apoptosis and Ferroptosis Pathway
title_full	Proteome Analysis of Camellia nitidissima Chi Revealed Its Role in Colon Cancer Through the Apoptosis and Ferroptosis Pathway
title_fullStr	Proteome Analysis of Camellia nitidissima Chi Revealed Its Role in Colon Cancer Through the Apoptosis and Ferroptosis Pathway
title_full_unstemmed	Proteome Analysis of Camellia nitidissima Chi Revealed Its Role in Colon Cancer Through the Apoptosis and Ferroptosis Pathway
title_sort	proteome analysis of camellia nitidissima chi revealed its role in colon cancer through the apoptosis and ferroptosis pathway
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/716397954dde46b0b7af505167a72da6
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Proteome Analysis of Camellia nitidissima Chi Revealed Its Role in Colon Cancer Through the Apoptosis and Ferroptosis Pathway

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