Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered

Abstract It is a fundamental question in cell biology and biophysics whether sphingomyelin (SM)- and cholesterol (Chol)- driven nanodomains exist in living cells and in model membranes. Biophysical studies on model membranes revealed SM and Chol driven micrometer-sized liquid-ordered domains. Althou...

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Autores principales:	Alena Koukalová, Mariana Amaro, Gokcan Aydogan, Gerhard Gröbner, Philip T. F. Williamson, Ilya Mikhalyov, Martin Hof, Radek Šachl
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7164659d8d9f4ac9aa6acda2fdde406c
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spelling	oai:doaj.org-article:7164659d8d9f4ac9aa6acda2fdde406c2021-12-02T11:52:36ZLipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered10.1038/s41598-017-05539-y2045-2322https://doaj.org/article/7164659d8d9f4ac9aa6acda2fdde406c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05539-yhttps://doaj.org/toc/2045-2322Abstract It is a fundamental question in cell biology and biophysics whether sphingomyelin (SM)- and cholesterol (Chol)- driven nanodomains exist in living cells and in model membranes. Biophysical studies on model membranes revealed SM and Chol driven micrometer-sized liquid-ordered domains. Although the existence of such microdomains has not been proven for the plasma membrane, such lipid mixtures have been often used as a model system for ‘rafts’. On the other hand, recent super resolution and single molecule results indicate that the plasma membrane might organize into nanocompartments. However, due to the limited resolution of those techniques their unambiguous characterization is still missing. In this work, a novel combination of Förster resonance energy transfer and Monte Carlo simulations (MC-FRET) identifies directly 10 nm large nanodomains in liquid-disordered model membranes composed of lipid mixtures containing SM and Chol. Combining MC-FRET with solid-state wide-line and high resolution magic angle spinning NMR as well as with fluorescence correlation spectroscopy we demonstrate that these nanodomains containing hundreds of lipid molecules are fluid and disordered. In terms of their size, fluidity, order and lifetime these nanodomains may represent a relevant model system for cellular membranes and are closely related to nanocompartments suggested to exist in cellular membranes.Alena KoukalováMariana AmaroGokcan AydoganGerhard GröbnerPhilip T. F. WilliamsonIlya MikhalyovMartin HofRadek ŠachlNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alena Koukalová Mariana Amaro Gokcan Aydogan Gerhard Gröbner Philip T. F. Williamson Ilya Mikhalyov Martin Hof Radek Šachl Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered
description	Abstract It is a fundamental question in cell biology and biophysics whether sphingomyelin (SM)- and cholesterol (Chol)- driven nanodomains exist in living cells and in model membranes. Biophysical studies on model membranes revealed SM and Chol driven micrometer-sized liquid-ordered domains. Although the existence of such microdomains has not been proven for the plasma membrane, such lipid mixtures have been often used as a model system for ‘rafts’. On the other hand, recent super resolution and single molecule results indicate that the plasma membrane might organize into nanocompartments. However, due to the limited resolution of those techniques their unambiguous characterization is still missing. In this work, a novel combination of Förster resonance energy transfer and Monte Carlo simulations (MC-FRET) identifies directly 10 nm large nanodomains in liquid-disordered model membranes composed of lipid mixtures containing SM and Chol. Combining MC-FRET with solid-state wide-line and high resolution magic angle spinning NMR as well as with fluorescence correlation spectroscopy we demonstrate that these nanodomains containing hundreds of lipid molecules are fluid and disordered. In terms of their size, fluidity, order and lifetime these nanodomains may represent a relevant model system for cellular membranes and are closely related to nanocompartments suggested to exist in cellular membranes.
format	article
author	Alena Koukalová Mariana Amaro Gokcan Aydogan Gerhard Gröbner Philip T. F. Williamson Ilya Mikhalyov Martin Hof Radek Šachl
author_facet	Alena Koukalová Mariana Amaro Gokcan Aydogan Gerhard Gröbner Philip T. F. Williamson Ilya Mikhalyov Martin Hof Radek Šachl
author_sort	Alena Koukalová
title	Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered
title_short	Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered
title_full	Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered
title_fullStr	Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered
title_full_unstemmed	Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered
title_sort	lipid driven nanodomains in giant lipid vesicles are fluid and disordered
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7164659d8d9f4ac9aa6acda2fdde406c
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Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered

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