Next generation plasma proteome profiling of COVID-19 patients with mild to moderate symptoms

Background: COVID-19 has caused millions of deaths globally, yet the cellular mechanisms underlying the various effects of the disease remain poorly understood. Recently, a new analytical platform for comprehensive analysis of plasma protein profiles using proximity extension assays combined with ne...

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Autores principales:	Wen Zhong, Ozlem Altay, Muhammad Arif, Fredrik Edfors, Levent Doganay, Adil Mardinoglu, Mathias Uhlen, Linn Fagerberg
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	COVID-19 Protein profiling Plasma proteome Immune response Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7164b72146b44fdc9e77d6fdcc3f62b6
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spelling	oai:doaj.org-article:7164b72146b44fdc9e77d6fdcc3f62b62021-11-28T04:33:30ZNext generation plasma proteome profiling of COVID-19 patients with mild to moderate symptoms2352-396410.1016/j.ebiom.2021.103723https://doaj.org/article/7164b72146b44fdc9e77d6fdcc3f62b62021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S235239642100517Xhttps://doaj.org/toc/2352-3964Background: COVID-19 has caused millions of deaths globally, yet the cellular mechanisms underlying the various effects of the disease remain poorly understood. Recently, a new analytical platform for comprehensive analysis of plasma protein profiles using proximity extension assays combined with next generation sequencing has been developed, which allows for multiple proteins to be analyzed simultaneously without sacrifice on accuracy or sensitivity. Methods: We analyzed the plasma protein profiles of COVID-19 patients (n = 50) with mild and moderate symptoms by comparing the protein levels in newly diagnosed patients with the protein levels in the same individuals after 14 days. Findings: The study has identified more than 200 proteins that are significantly elevated during infection and many of these are related to cytokine response and other immune-related functions. In addition, several other proteins are shown to be elevated, including SCARB2, a host cell receptor protein involved in virus entry. A comparison with the plasma protein response in patients with severe symptoms shows a highly similar pattern, but with some interesting differences. Interpretation: The study presented here demonstrates the usefulness of “next generation plasma protein profiling” to identify molecular signatures of importance for disease progression and to allow monitoring of disease during recovery from the infection. The results will facilitate further studies to understand the molecular mechanism of the immune-related response of the SARS-CoV-2 virus. Funding: This work was financially supported by Knut and Alice Wallenberg Foundation.Wen ZhongOzlem AltayMuhammad ArifFredrik EdforsLevent DoganayAdil MardinogluMathias UhlenLinn FagerbergElsevierarticleCOVID-19Protein profilingPlasma proteomeImmune responseMedicineRMedicine (General)R5-920ENEBioMedicine, Vol 74, Iss , Pp 103723- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	COVID-19 Protein profiling Plasma proteome Immune response Medicine R Medicine (General) R5-920
spellingShingle	COVID-19 Protein profiling Plasma proteome Immune response Medicine R Medicine (General) R5-920 Wen Zhong Ozlem Altay Muhammad Arif Fredrik Edfors Levent Doganay Adil Mardinoglu Mathias Uhlen Linn Fagerberg Next generation plasma proteome profiling of COVID-19 patients with mild to moderate symptoms
description	Background: COVID-19 has caused millions of deaths globally, yet the cellular mechanisms underlying the various effects of the disease remain poorly understood. Recently, a new analytical platform for comprehensive analysis of plasma protein profiles using proximity extension assays combined with next generation sequencing has been developed, which allows for multiple proteins to be analyzed simultaneously without sacrifice on accuracy or sensitivity. Methods: We analyzed the plasma protein profiles of COVID-19 patients (n = 50) with mild and moderate symptoms by comparing the protein levels in newly diagnosed patients with the protein levels in the same individuals after 14 days. Findings: The study has identified more than 200 proteins that are significantly elevated during infection and many of these are related to cytokine response and other immune-related functions. In addition, several other proteins are shown to be elevated, including SCARB2, a host cell receptor protein involved in virus entry. A comparison with the plasma protein response in patients with severe symptoms shows a highly similar pattern, but with some interesting differences. Interpretation: The study presented here demonstrates the usefulness of “next generation plasma protein profiling” to identify molecular signatures of importance for disease progression and to allow monitoring of disease during recovery from the infection. The results will facilitate further studies to understand the molecular mechanism of the immune-related response of the SARS-CoV-2 virus. Funding: This work was financially supported by Knut and Alice Wallenberg Foundation.
format	article
author	Wen Zhong Ozlem Altay Muhammad Arif Fredrik Edfors Levent Doganay Adil Mardinoglu Mathias Uhlen Linn Fagerberg
author_facet	Wen Zhong Ozlem Altay Muhammad Arif Fredrik Edfors Levent Doganay Adil Mardinoglu Mathias Uhlen Linn Fagerberg
author_sort	Wen Zhong
title	Next generation plasma proteome profiling of COVID-19 patients with mild to moderate symptoms
title_short	Next generation plasma proteome profiling of COVID-19 patients with mild to moderate symptoms
title_full	Next generation plasma proteome profiling of COVID-19 patients with mild to moderate symptoms
title_fullStr	Next generation plasma proteome profiling of COVID-19 patients with mild to moderate symptoms
title_full_unstemmed	Next generation plasma proteome profiling of COVID-19 patients with mild to moderate symptoms
title_sort	next generation plasma proteome profiling of covid-19 patients with mild to moderate symptoms
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/7164b72146b44fdc9e77d6fdcc3f62b6
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Next generation plasma proteome profiling of COVID-19 patients with mild to moderate symptoms

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