In Vivo Molecular Optical Coherence Tomography of Lymphatic Vessel Endothelial Hyaluronan Receptors

Abstract Optical Coherence Tomography (OCT) imaging of living subjects offers increased depth of penetration while maintaining high spatial resolution when compared to other optical microscopy techniques. However, since most protein biomarkers do not exhibit inherent contrast detectable by OCT, exog...

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Autores principales:	Peng Si, Debasish Sen, Rebecca Dutta, Siavash Yousefi, Roopa Dalal, Yonatan Winetraub, Orly Liba, Adam de la Zerda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/716ae97445a14025ac104e3e1c9c3eb2
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spelling	oai:doaj.org-article:716ae97445a14025ac104e3e1c9c3eb22021-12-02T12:32:25ZIn Vivo Molecular Optical Coherence Tomography of Lymphatic Vessel Endothelial Hyaluronan Receptors10.1038/s41598-017-01172-x2045-2322https://doaj.org/article/716ae97445a14025ac104e3e1c9c3eb22017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01172-xhttps://doaj.org/toc/2045-2322Abstract Optical Coherence Tomography (OCT) imaging of living subjects offers increased depth of penetration while maintaining high spatial resolution when compared to other optical microscopy techniques. However, since most protein biomarkers do not exhibit inherent contrast detectable by OCT, exogenous contrast agents must be employed for imaging specific cellular biomarkers of interest. While a number of OCT contrast agents have been previously studied, demonstrations of molecular targeting with such agents in live animals have been historically challenging and notably limited in success. Here we demonstrate for the first time that microbeads (µBs) can be used as contrast agents to target cellular biomarkers in lymphatic vessels and can be detected by OCT using a phase variance algorithm. This molecular OCT method enables in vivo imaging of the expression profiles of lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1), a biomarker that plays crucial roles in inflammation and tumor metastasis. In vivo OCT imaging of LVYE-1 showed that the biomarker was significantly down-regulated during inflammation induced by acute contact hypersensitivity (CHS). Our work demonstrated a powerful molecular imaging tool that can be used for high resolution studies of lymphatic function and dynamics in models of inflammation, tumor development, and other lymphatic diseases.Peng SiDebasish SenRebecca DuttaSiavash YousefiRoopa DalalYonatan WinetraubOrly LibaAdam de la ZerdaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peng Si Debasish Sen Rebecca Dutta Siavash Yousefi Roopa Dalal Yonatan Winetraub Orly Liba Adam de la Zerda In Vivo Molecular Optical Coherence Tomography of Lymphatic Vessel Endothelial Hyaluronan Receptors
description	Abstract Optical Coherence Tomography (OCT) imaging of living subjects offers increased depth of penetration while maintaining high spatial resolution when compared to other optical microscopy techniques. However, since most protein biomarkers do not exhibit inherent contrast detectable by OCT, exogenous contrast agents must be employed for imaging specific cellular biomarkers of interest. While a number of OCT contrast agents have been previously studied, demonstrations of molecular targeting with such agents in live animals have been historically challenging and notably limited in success. Here we demonstrate for the first time that microbeads (µBs) can be used as contrast agents to target cellular biomarkers in lymphatic vessels and can be detected by OCT using a phase variance algorithm. This molecular OCT method enables in vivo imaging of the expression profiles of lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1), a biomarker that plays crucial roles in inflammation and tumor metastasis. In vivo OCT imaging of LVYE-1 showed that the biomarker was significantly down-regulated during inflammation induced by acute contact hypersensitivity (CHS). Our work demonstrated a powerful molecular imaging tool that can be used for high resolution studies of lymphatic function and dynamics in models of inflammation, tumor development, and other lymphatic diseases.
format	article
author	Peng Si Debasish Sen Rebecca Dutta Siavash Yousefi Roopa Dalal Yonatan Winetraub Orly Liba Adam de la Zerda
author_facet	Peng Si Debasish Sen Rebecca Dutta Siavash Yousefi Roopa Dalal Yonatan Winetraub Orly Liba Adam de la Zerda
author_sort	Peng Si
title	In Vivo Molecular Optical Coherence Tomography of Lymphatic Vessel Endothelial Hyaluronan Receptors
title_short	In Vivo Molecular Optical Coherence Tomography of Lymphatic Vessel Endothelial Hyaluronan Receptors
title_full	In Vivo Molecular Optical Coherence Tomography of Lymphatic Vessel Endothelial Hyaluronan Receptors
title_fullStr	In Vivo Molecular Optical Coherence Tomography of Lymphatic Vessel Endothelial Hyaluronan Receptors
title_full_unstemmed	In Vivo Molecular Optical Coherence Tomography of Lymphatic Vessel Endothelial Hyaluronan Receptors
title_sort	in vivo molecular optical coherence tomography of lymphatic vessel endothelial hyaluronan receptors
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/716ae97445a14025ac104e3e1c9c3eb2
work_keys_str_mv	AT pengsi invivomolecularopticalcoherencetomographyoflymphaticvesselendothelialhyaluronanreceptors AT debasishsen invivomolecularopticalcoherencetomographyoflymphaticvesselendothelialhyaluronanreceptors AT rebeccadutta invivomolecularopticalcoherencetomographyoflymphaticvesselendothelialhyaluronanreceptors AT siavashyousefi invivomolecularopticalcoherencetomographyoflymphaticvesselendothelialhyaluronanreceptors AT roopadalal invivomolecularopticalcoherencetomographyoflymphaticvesselendothelialhyaluronanreceptors AT yonatanwinetraub invivomolecularopticalcoherencetomographyoflymphaticvesselendothelialhyaluronanreceptors AT orlyliba invivomolecularopticalcoherencetomographyoflymphaticvesselendothelialhyaluronanreceptors AT adamdelazerda invivomolecularopticalcoherencetomographyoflymphaticvesselendothelialhyaluronanreceptors
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In Vivo Molecular Optical Coherence Tomography of Lymphatic Vessel Endothelial Hyaluronan Receptors

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