Reduced proliferation in the adult mouse subventricular zone increases survival of olfactory bulb interneurons.

Neurogenesis in the adult brain is largely restricted to the subependymal zone (SVZ) of the lateral ventricle, olfactory bulb (OB) and the dentate subgranular zone, and survival of adult-born cells in the OB is influenced by factors including sensory experience. We examined, in mice, whether surviva...

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Autores principales:	Yi Sui, Malcolm K Horne, Davor Stanić
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/716b538a0bd2482398cfb2fc9e9d580f
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spelling	oai:doaj.org-article:716b538a0bd2482398cfb2fc9e9d580f2021-11-18T07:27:20ZReduced proliferation in the adult mouse subventricular zone increases survival of olfactory bulb interneurons.1932-620310.1371/journal.pone.0031549https://doaj.org/article/716b538a0bd2482398cfb2fc9e9d580f2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22363671/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Neurogenesis in the adult brain is largely restricted to the subependymal zone (SVZ) of the lateral ventricle, olfactory bulb (OB) and the dentate subgranular zone, and survival of adult-born cells in the OB is influenced by factors including sensory experience. We examined, in mice, whether survival of adult-born cells is also regulated by the rate of precursor proliferation in the SVZ. Precursor proliferation was decreased by depleting the SVZ of dopamine after lesioning dopamine neurons in the substantia nigra compacta with 6-hydroxydopamine. Subsequently, we examined the effect of reduced SVZ proliferation on the generation, migration and survival of neuroblasts and mature adult-born cells in the SVZ, rostral migratory stream (RMS) and OB. Proliferating cells in the SVZ, measured by 5-bromo-2-deoxyuridine (BrdU) injected 2 hours prior to death or by immunoreactivity against Ki67, were reduced by 47% or 36%, respectively, 7 days after dopamine depletion, and by 29% or 31% 42 days after dopamine depletion, compared to sham-treated animals. Neuroblast generation in the SVZ and their migration along the RMS were not affected, neither 7 nor 42 days after the 6-hydroxydopamine injection, since the number of doublecortin-immunoreactive neuroblasts in the SVZ and RMS, as well as the number of neuronal nuclei-immunoreactive cells in the OB, were stable compared to control. However, survival analysis 15 days after 6-hydroxydopamine and 6 days after BrdU injections showed that the number of BrdU+ cells in the SVZ was 70% higher. Also, 42 days after 6-hydroxydopamine and 30 days after BrdU injections, we found an 82% increase in co-labeled BrdU+/γ-aminobutyric acid-immunoreactive cell bodies in the granular cell layer, while double-labeled BrdU+/tyrosine hydroxylase-immunoreactive cell bodies in the glomerular layer increased by 148%. We conclude that the number of OB interneurons following reduced SVZ proliferation is maintained through an increased survival of adult-born precursor cells, neuroblasts and interneurons.Yi SuiMalcolm K HorneDavor StanićPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31549 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi Sui Malcolm K Horne Davor Stanić Reduced proliferation in the adult mouse subventricular zone increases survival of olfactory bulb interneurons.
description	Neurogenesis in the adult brain is largely restricted to the subependymal zone (SVZ) of the lateral ventricle, olfactory bulb (OB) and the dentate subgranular zone, and survival of adult-born cells in the OB is influenced by factors including sensory experience. We examined, in mice, whether survival of adult-born cells is also regulated by the rate of precursor proliferation in the SVZ. Precursor proliferation was decreased by depleting the SVZ of dopamine after lesioning dopamine neurons in the substantia nigra compacta with 6-hydroxydopamine. Subsequently, we examined the effect of reduced SVZ proliferation on the generation, migration and survival of neuroblasts and mature adult-born cells in the SVZ, rostral migratory stream (RMS) and OB. Proliferating cells in the SVZ, measured by 5-bromo-2-deoxyuridine (BrdU) injected 2 hours prior to death or by immunoreactivity against Ki67, were reduced by 47% or 36%, respectively, 7 days after dopamine depletion, and by 29% or 31% 42 days after dopamine depletion, compared to sham-treated animals. Neuroblast generation in the SVZ and their migration along the RMS were not affected, neither 7 nor 42 days after the 6-hydroxydopamine injection, since the number of doublecortin-immunoreactive neuroblasts in the SVZ and RMS, as well as the number of neuronal nuclei-immunoreactive cells in the OB, were stable compared to control. However, survival analysis 15 days after 6-hydroxydopamine and 6 days after BrdU injections showed that the number of BrdU+ cells in the SVZ was 70% higher. Also, 42 days after 6-hydroxydopamine and 30 days after BrdU injections, we found an 82% increase in co-labeled BrdU+/γ-aminobutyric acid-immunoreactive cell bodies in the granular cell layer, while double-labeled BrdU+/tyrosine hydroxylase-immunoreactive cell bodies in the glomerular layer increased by 148%. We conclude that the number of OB interneurons following reduced SVZ proliferation is maintained through an increased survival of adult-born precursor cells, neuroblasts and interneurons.
format	article
author	Yi Sui Malcolm K Horne Davor Stanić
author_facet	Yi Sui Malcolm K Horne Davor Stanić
author_sort	Yi Sui
title	Reduced proliferation in the adult mouse subventricular zone increases survival of olfactory bulb interneurons.
title_short	Reduced proliferation in the adult mouse subventricular zone increases survival of olfactory bulb interneurons.
title_full	Reduced proliferation in the adult mouse subventricular zone increases survival of olfactory bulb interneurons.
title_fullStr	Reduced proliferation in the adult mouse subventricular zone increases survival of olfactory bulb interneurons.
title_full_unstemmed	Reduced proliferation in the adult mouse subventricular zone increases survival of olfactory bulb interneurons.
title_sort	reduced proliferation in the adult mouse subventricular zone increases survival of olfactory bulb interneurons.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/716b538a0bd2482398cfb2fc9e9d580f
work_keys_str_mv	AT yisui reducedproliferationintheadultmousesubventricularzoneincreasessurvivalofolfactorybulbinterneurons AT malcolmkhorne reducedproliferationintheadultmousesubventricularzoneincreasessurvivalofolfactorybulbinterneurons AT davorstanic reducedproliferationintheadultmousesubventricularzoneincreasessurvivalofolfactorybulbinterneurons
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Reduced proliferation in the adult mouse subventricular zone increases survival of olfactory bulb interneurons.

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