Bacillus subtilis two-component system sensory kinase DegS is regulated by serine phosphorylation in its input domain.

Bacillus subtilis two-component system DegS/U is well known for the complexity of its regulation. The cytosolic sensory kinase DegS does not receive a single predominant input signal like most two-component kinases, instead it integrates a wide array of metabolic inputs that modulate its activity. T...

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Autores principales:	Carsten Jers, Ahasanul Kobir, Elsebeth Oline Søndergaard, Peter Ruhdal Jensen, Ivan Mijakovic
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/716e4e6917ea4a88b553662b39aec58e
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spelling	oai:doaj.org-article:716e4e6917ea4a88b553662b39aec58e2021-11-18T06:59:15ZBacillus subtilis two-component system sensory kinase DegS is regulated by serine phosphorylation in its input domain.1932-620310.1371/journal.pone.0014653https://doaj.org/article/716e4e6917ea4a88b553662b39aec58e2011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21304896/?tool=EBIhttps://doaj.org/toc/1932-6203Bacillus subtilis two-component system DegS/U is well known for the complexity of its regulation. The cytosolic sensory kinase DegS does not receive a single predominant input signal like most two-component kinases, instead it integrates a wide array of metabolic inputs that modulate its activity. The phosphorylation state of the response regulator DegU also does not confer a straightforward "on/off" response; it is fine-tuned and at different levels triggers different sub-regulons. Here we describe serine phosphorylation of the DegS sensing domain, which stimulates its kinase activity. We demonstrate that DegS phosphorylation can be carried out by at least two B. subtilis Hanks-type kinases in vitro, and this stimulates the phosphate transfer towards DegU. The consequences of this process were studied in vivo, using phosphomimetic (Ser76Asp) and non-phosphorylatable (Ser76Ala) mutants of DegS. In a number of physiological assays focused on different processes regulated by DegU, DegS S76D phosphomimetic mutant behaved like a strain with intermediate levels of DegU phosphorylation, whereas DegS S76A behaved like a strain with lower levels of DegU phophorylation. These findings suggest a link between DegS phosphorylation at serine 76 and the level of DegU phosphorylation, establishing this post-translational modification as an additional trigger for this two-component system.Carsten JersAhasanul KobirElsebeth Oline SøndergaardPeter Ruhdal JensenIvan MijakovicPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e14653 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carsten Jers Ahasanul Kobir Elsebeth Oline Søndergaard Peter Ruhdal Jensen Ivan Mijakovic Bacillus subtilis two-component system sensory kinase DegS is regulated by serine phosphorylation in its input domain.
description	Bacillus subtilis two-component system DegS/U is well known for the complexity of its regulation. The cytosolic sensory kinase DegS does not receive a single predominant input signal like most two-component kinases, instead it integrates a wide array of metabolic inputs that modulate its activity. The phosphorylation state of the response regulator DegU also does not confer a straightforward "on/off" response; it is fine-tuned and at different levels triggers different sub-regulons. Here we describe serine phosphorylation of the DegS sensing domain, which stimulates its kinase activity. We demonstrate that DegS phosphorylation can be carried out by at least two B. subtilis Hanks-type kinases in vitro, and this stimulates the phosphate transfer towards DegU. The consequences of this process were studied in vivo, using phosphomimetic (Ser76Asp) and non-phosphorylatable (Ser76Ala) mutants of DegS. In a number of physiological assays focused on different processes regulated by DegU, DegS S76D phosphomimetic mutant behaved like a strain with intermediate levels of DegU phosphorylation, whereas DegS S76A behaved like a strain with lower levels of DegU phophorylation. These findings suggest a link between DegS phosphorylation at serine 76 and the level of DegU phosphorylation, establishing this post-translational modification as an additional trigger for this two-component system.
format	article
author	Carsten Jers Ahasanul Kobir Elsebeth Oline Søndergaard Peter Ruhdal Jensen Ivan Mijakovic
author_facet	Carsten Jers Ahasanul Kobir Elsebeth Oline Søndergaard Peter Ruhdal Jensen Ivan Mijakovic
author_sort	Carsten Jers
title	Bacillus subtilis two-component system sensory kinase DegS is regulated by serine phosphorylation in its input domain.
title_short	Bacillus subtilis two-component system sensory kinase DegS is regulated by serine phosphorylation in its input domain.
title_full	Bacillus subtilis two-component system sensory kinase DegS is regulated by serine phosphorylation in its input domain.
title_fullStr	Bacillus subtilis two-component system sensory kinase DegS is regulated by serine phosphorylation in its input domain.
title_full_unstemmed	Bacillus subtilis two-component system sensory kinase DegS is regulated by serine phosphorylation in its input domain.
title_sort	bacillus subtilis two-component system sensory kinase degs is regulated by serine phosphorylation in its input domain.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/716e4e6917ea4a88b553662b39aec58e
work_keys_str_mv	AT carstenjers bacillussubtilistwocomponentsystemsensorykinasedegsisregulatedbyserinephosphorylationinitsinputdomain AT ahasanulkobir bacillussubtilistwocomponentsystemsensorykinasedegsisregulatedbyserinephosphorylationinitsinputdomain AT elsebetholinesøndergaard bacillussubtilistwocomponentsystemsensorykinasedegsisregulatedbyserinephosphorylationinitsinputdomain AT peterruhdaljensen bacillussubtilistwocomponentsystemsensorykinasedegsisregulatedbyserinephosphorylationinitsinputdomain AT ivanmijakovic bacillussubtilistwocomponentsystemsensorykinasedegsisregulatedbyserinephosphorylationinitsinputdomain
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Bacillus subtilis two-component system sensory kinase DegS is regulated by serine phosphorylation in its input domain.

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