Identification of glucose transporters in Aspergillus nidulans.

To characterize the mechanisms involved in glucose transport, in the filamentous fungus Aspergillus nidulans, we have identified four glucose transporter encoding genes hxtB-E. We evaluated the ability of hxtB-E to functionally complement the Saccharomyces cerevisiae EBY.VW4000 strain that is unable...

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Autores principales:	Thaila Fernanda Dos Reis, João Filipe Menino, Vinícius Leite Pedro Bom, Neil Andrew Brown, Ana Cristina Colabardini, Marcela Savoldi, Maria Helena S Goldman, Fernando Rodrigues, Gustavo Henrique Goldman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/71704d88f58942eeb62c5642d8149e27
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spelling	oai:doaj.org-article:71704d88f58942eeb62c5642d8149e272021-11-18T08:44:48ZIdentification of glucose transporters in Aspergillus nidulans.1932-620310.1371/journal.pone.0081412https://doaj.org/article/71704d88f58942eeb62c5642d8149e272013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24282591/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203To characterize the mechanisms involved in glucose transport, in the filamentous fungus Aspergillus nidulans, we have identified four glucose transporter encoding genes hxtB-E. We evaluated the ability of hxtB-E to functionally complement the Saccharomyces cerevisiae EBY.VW4000 strain that is unable to grow on glucose, fructose, mannose or galactose as single carbon source. In S. cerevisiae HxtB-E were targeted to the plasma membrane. The expression of HxtB, HxtC and HxtE was able to restore growth on glucose, fructose, mannose or galactose, indicating that these transporters accept multiple sugars as a substrate through an energy dependent process. A tenfold excess of unlabeled maltose, galactose, fructose, and mannose were able to inhibit glucose uptake to different levels (50 to 80 %) in these s. cerevisiae complemented strains. Moreover, experiments with cyanide-m-chlorophenylhydrazone (CCCP), strongly suggest that hxtB, -C, and -E mediate glucose transport via active proton symport. The A. nidulans ΔhxtB, ΔhxtC or ΔhxtE null mutants showed ~2.5-fold reduction in the affinity for glucose, while ΔhxtB and -C also showed a 2-fold reduction in the capacity for glucose uptake. The ΔhxtD mutant had a 7.8-fold reduction in affinity, but a 3-fold increase in the capacity for glucose uptake. However, only the ΔhxtB mutant strain showed a detectable decreased rate of glucose consumption at low concentrations and an increased resistance to 2-deoxyglucose.Thaila Fernanda Dos ReisJoão Filipe MeninoVinícius Leite Pedro BomNeil Andrew BrownAna Cristina ColabardiniMarcela SavoldiMaria Helena S GoldmanFernando RodriguesGustavo Henrique GoldmanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e81412 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thaila Fernanda Dos Reis João Filipe Menino Vinícius Leite Pedro Bom Neil Andrew Brown Ana Cristina Colabardini Marcela Savoldi Maria Helena S Goldman Fernando Rodrigues Gustavo Henrique Goldman Identification of glucose transporters in Aspergillus nidulans.
description	To characterize the mechanisms involved in glucose transport, in the filamentous fungus Aspergillus nidulans, we have identified four glucose transporter encoding genes hxtB-E. We evaluated the ability of hxtB-E to functionally complement the Saccharomyces cerevisiae EBY.VW4000 strain that is unable to grow on glucose, fructose, mannose or galactose as single carbon source. In S. cerevisiae HxtB-E were targeted to the plasma membrane. The expression of HxtB, HxtC and HxtE was able to restore growth on glucose, fructose, mannose or galactose, indicating that these transporters accept multiple sugars as a substrate through an energy dependent process. A tenfold excess of unlabeled maltose, galactose, fructose, and mannose were able to inhibit glucose uptake to different levels (50 to 80 %) in these s. cerevisiae complemented strains. Moreover, experiments with cyanide-m-chlorophenylhydrazone (CCCP), strongly suggest that hxtB, -C, and -E mediate glucose transport via active proton symport. The A. nidulans ΔhxtB, ΔhxtC or ΔhxtE null mutants showed ~2.5-fold reduction in the affinity for glucose, while ΔhxtB and -C also showed a 2-fold reduction in the capacity for glucose uptake. The ΔhxtD mutant had a 7.8-fold reduction in affinity, but a 3-fold increase in the capacity for glucose uptake. However, only the ΔhxtB mutant strain showed a detectable decreased rate of glucose consumption at low concentrations and an increased resistance to 2-deoxyglucose.
format	article
author	Thaila Fernanda Dos Reis João Filipe Menino Vinícius Leite Pedro Bom Neil Andrew Brown Ana Cristina Colabardini Marcela Savoldi Maria Helena S Goldman Fernando Rodrigues Gustavo Henrique Goldman
author_facet	Thaila Fernanda Dos Reis João Filipe Menino Vinícius Leite Pedro Bom Neil Andrew Brown Ana Cristina Colabardini Marcela Savoldi Maria Helena S Goldman Fernando Rodrigues Gustavo Henrique Goldman
author_sort	Thaila Fernanda Dos Reis
title	Identification of glucose transporters in Aspergillus nidulans.
title_short	Identification of glucose transporters in Aspergillus nidulans.
title_full	Identification of glucose transporters in Aspergillus nidulans.
title_fullStr	Identification of glucose transporters in Aspergillus nidulans.
title_full_unstemmed	Identification of glucose transporters in Aspergillus nidulans.
title_sort	identification of glucose transporters in aspergillus nidulans.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/71704d88f58942eeb62c5642d8149e27
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Identification of glucose transporters in Aspergillus nidulans.

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