BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA

The emergence of resistance among the most important bacterial pathogens is generally recognized as one of the major public health problems. The most important of these organisms are penicillin-resistant Streptococcus pneumoniae, vancomycin-resistant enterococci and methicillin- and vancomycin-resis...

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Autores principales:	A. Yu. Borisenko, Yu. P. Dzhioev, N. P. Peretolchina, L. A. Stepanenko, V. A. Kuzminova, Yu. M. Zemlyanskaya, L. A. Kokorina, N. A. Arefieva, O. N. Reva, Y. Wang, Z. Qu, V. I. Zlobin
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2018
Materias:	crispr/casсистема Science Q
Acceso en línea:	https://doaj.org/article/7172ef98e787428b9ecc43e4d8acfdb1
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spelling	oai:doaj.org-article:7172ef98e787428b9ecc43e4d8acfdb12021-11-23T06:14:41ZBIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA2541-94202587-959610.29413/ABS.2018-3.5.7https://doaj.org/article/7172ef98e787428b9ecc43e4d8acfdb12018-09-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/706https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596The emergence of resistance among the most important bacterial pathogens is generally recognized as one of the major public health problems. The most important of these organisms are penicillin-resistant Streptococcus pneumoniae, vancomycin-resistant enterococci and methicillin- and vancomycin-resistant Staphylococcus aureus. These antibiotic resistance in common pathogens have made antimicrobial therapy of many infections. Scientists need to look for new ways of treating bacterial infections in the work, using the developed algorithm from the methods of search software in the genomic structure of Staphylococcus aureus subsp. aureus ST228, the CRISPR/Cas locus and the division structures of its CRISPR cassette. The results of the bacteriophage search through the decoded spacer sequences of CRISPR-cassettes of this strain were also obtained using the developed algorithm of the software methods of bioinformatics. It was determined that the CRISPR/Cas system of strain of ST228 of S. aureus was of type IIIA. It is shown that cas-genes are in the immediate vicinity of CRISPR cassettes. The spacer structures in the detected CRISPR cassette are the Staphylococcus, Mycobacterium, Streptococcus, Bacillus, Gordonia, Arthrobacter, Streptomyces. The implementation of the algorithm of program methods for locating CRISPR/Cas-loci can be applied to many other decoded bacterial genomes to return bacteriophage therapy.A. Yu. BorisenkoYu. P. DzhioevN. P. PeretolchinaL. A. StepanenkoV. A. KuzminovaYu. M. ZemlyanskayaL. A. KokorinaN. A. ArefievaO. N. RevaY. WangZ. QuV. I. ZlobinScientific Сentre for Family Health and Human Reproduction Problemsarticlecrispr/casсистемаScienceQRUActa Biomedica Scientifica, Vol 3, Iss 5, Pp 49-53 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	crispr/casсистема Science Q
spellingShingle	crispr/casсистема Science Q A. Yu. Borisenko Yu. P. Dzhioev N. P. Peretolchina L. A. Stepanenko V. A. Kuzminova Yu. M. Zemlyanskaya L. A. Kokorina N. A. Arefieva O. N. Reva Y. Wang Z. Qu V. I. Zlobin BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA
description	The emergence of resistance among the most important bacterial pathogens is generally recognized as one of the major public health problems. The most important of these organisms are penicillin-resistant Streptococcus pneumoniae, vancomycin-resistant enterococci and methicillin- and vancomycin-resistant Staphylococcus aureus. These antibiotic resistance in common pathogens have made antimicrobial therapy of many infections. Scientists need to look for new ways of treating bacterial infections in the work, using the developed algorithm from the methods of search software in the genomic structure of Staphylococcus aureus subsp. aureus ST228, the CRISPR/Cas locus and the division structures of its CRISPR cassette. The results of the bacteriophage search through the decoded spacer sequences of CRISPR-cassettes of this strain were also obtained using the developed algorithm of the software methods of bioinformatics. It was determined that the CRISPR/Cas system of strain of ST228 of S. aureus was of type IIIA. It is shown that cas-genes are in the immediate vicinity of CRISPR cassettes. The spacer structures in the detected CRISPR cassette are the Staphylococcus, Mycobacterium, Streptococcus, Bacillus, Gordonia, Arthrobacter, Streptomyces. The implementation of the algorithm of program methods for locating CRISPR/Cas-loci can be applied to many other decoded bacterial genomes to return bacteriophage therapy.
format	article
author	A. Yu. Borisenko Yu. P. Dzhioev N. P. Peretolchina L. A. Stepanenko V. A. Kuzminova Yu. M. Zemlyanskaya L. A. Kokorina N. A. Arefieva O. N. Reva Y. Wang Z. Qu V. I. Zlobin
author_facet	A. Yu. Borisenko Yu. P. Dzhioev N. P. Peretolchina L. A. Stepanenko V. A. Kuzminova Yu. M. Zemlyanskaya L. A. Kokorina N. A. Arefieva O. N. Reva Y. Wang Z. Qu V. I. Zlobin
author_sort	A. Yu. Borisenko
title	BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA
title_short	BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA
title_full	BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA
title_fullStr	BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA
title_full_unstemmed	BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA
title_sort	bioinformation search and analysis of structures of crispr/cas systems in phage staphylococus aureus genome and estimation of profiles of phage detected through crispr-cassette bacteria
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2018
url	https://doaj.org/article/7172ef98e787428b9ecc43e4d8acfdb1
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BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA

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