The RNA binding protein ESRP1 fine-tunes the expression of pluripotency-related factors in mouse embryonic stem cells.

In pluripotent stem cells, there is increasing evidence for crosstalk between post-transcriptional and transcriptional networks, offering multifold steps at which pluripotency can be controlled. In addition to well-studied transcription factors, chromatin modifiers and miRNAs, RNA-binding proteins a...

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Autores principales:	Sharmila Fagoonee, Claudia Bearzi, Ferdinando Di Cunto, John G Clohessy, Roberto Rizzi, Markus Reschke, Emanuela Tolosano, Paolo Provero, Pier Paolo Pandolfi, Lorenzo Silengo, Fiorella Altruda
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7175660abb2249f590890fd252ab86ae
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spelling	oai:doaj.org-article:7175660abb2249f590890fd252ab86ae2021-11-18T08:58:00ZThe RNA binding protein ESRP1 fine-tunes the expression of pluripotency-related factors in mouse embryonic stem cells.1932-620310.1371/journal.pone.0072300https://doaj.org/article/7175660abb2249f590890fd252ab86ae2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24015231/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In pluripotent stem cells, there is increasing evidence for crosstalk between post-transcriptional and transcriptional networks, offering multifold steps at which pluripotency can be controlled. In addition to well-studied transcription factors, chromatin modifiers and miRNAs, RNA-binding proteins are emerging as fundamental players in pluripotency regulation. Here, we report a new role for the RNA-binding protein ESRP1 in the control of pluripotency. Knockdown of Esrp1 in mouse embryonic stem cells induces, other than the well-documented epithelial to mesenchymal-like state, also an increase in expression of the core transcription factors Oct4, Nanog and Sox2, thereby enhancing self-renewal of these cells. Esrp1-depleted embryonic stem cells displayed impaired early differentiation in vitro and formed larger teratomas in vivo when compared to control embryonic stem cells. We also show that ESRP1 binds to Oct4 and Sox2 mRNAs and decreases their polysomal loading. ESRP1 thus acts as a physiological regulator of the finely-tuned balance between self-renewal and commitment to a restricted developmental fate. Importantly, both mouse and human epithelial stem cells highly express ESRP1, pinpointing the importance of this RNA-binding protein in stem cell biology.Sharmila FagooneeClaudia BearziFerdinando Di CuntoJohn G ClohessyRoberto RizziMarkus ReschkeEmanuela TolosanoPaolo ProveroPier Paolo PandolfiLorenzo SilengoFiorella AltrudaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e72300 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sharmila Fagoonee Claudia Bearzi Ferdinando Di Cunto John G Clohessy Roberto Rizzi Markus Reschke Emanuela Tolosano Paolo Provero Pier Paolo Pandolfi Lorenzo Silengo Fiorella Altruda The RNA binding protein ESRP1 fine-tunes the expression of pluripotency-related factors in mouse embryonic stem cells.
description	In pluripotent stem cells, there is increasing evidence for crosstalk between post-transcriptional and transcriptional networks, offering multifold steps at which pluripotency can be controlled. In addition to well-studied transcription factors, chromatin modifiers and miRNAs, RNA-binding proteins are emerging as fundamental players in pluripotency regulation. Here, we report a new role for the RNA-binding protein ESRP1 in the control of pluripotency. Knockdown of Esrp1 in mouse embryonic stem cells induces, other than the well-documented epithelial to mesenchymal-like state, also an increase in expression of the core transcription factors Oct4, Nanog and Sox2, thereby enhancing self-renewal of these cells. Esrp1-depleted embryonic stem cells displayed impaired early differentiation in vitro and formed larger teratomas in vivo when compared to control embryonic stem cells. We also show that ESRP1 binds to Oct4 and Sox2 mRNAs and decreases their polysomal loading. ESRP1 thus acts as a physiological regulator of the finely-tuned balance between self-renewal and commitment to a restricted developmental fate. Importantly, both mouse and human epithelial stem cells highly express ESRP1, pinpointing the importance of this RNA-binding protein in stem cell biology.
format	article
author	Sharmila Fagoonee Claudia Bearzi Ferdinando Di Cunto John G Clohessy Roberto Rizzi Markus Reschke Emanuela Tolosano Paolo Provero Pier Paolo Pandolfi Lorenzo Silengo Fiorella Altruda
author_facet	Sharmila Fagoonee Claudia Bearzi Ferdinando Di Cunto John G Clohessy Roberto Rizzi Markus Reschke Emanuela Tolosano Paolo Provero Pier Paolo Pandolfi Lorenzo Silengo Fiorella Altruda
author_sort	Sharmila Fagoonee
title	The RNA binding protein ESRP1 fine-tunes the expression of pluripotency-related factors in mouse embryonic stem cells.
title_short	The RNA binding protein ESRP1 fine-tunes the expression of pluripotency-related factors in mouse embryonic stem cells.
title_full	The RNA binding protein ESRP1 fine-tunes the expression of pluripotency-related factors in mouse embryonic stem cells.
title_fullStr	The RNA binding protein ESRP1 fine-tunes the expression of pluripotency-related factors in mouse embryonic stem cells.
title_full_unstemmed	The RNA binding protein ESRP1 fine-tunes the expression of pluripotency-related factors in mouse embryonic stem cells.
title_sort	rna binding protein esrp1 fine-tunes the expression of pluripotency-related factors in mouse embryonic stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7175660abb2249f590890fd252ab86ae
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The RNA binding protein ESRP1 fine-tunes the expression of pluripotency-related factors in mouse embryonic stem cells.

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