Global gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosa

Abstract The anuran Rhinella spinulosa is distributed along the Andes Range at altitudes that undergo wide daily and seasonal variation in temperature. One of the populations inhabits geothermal streams, a stable environment that influences life history traits such as the timing of metamorphosis. To...

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Autores principales:	Luis Pastenes, Camilo Valdivieso, Alex Di Genova, Dante Travisany, Andrew Hart, Martín Montecino, Ariel Orellana, Mauricio Gonzalez, Rodrigo A. Gutiérrez, Miguel L. Allende, Alejandro Maass, Marco A. Méndez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7177982607b944ec803bc442d686a776
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spelling	oai:doaj.org-article:7177982607b944ec803bc442d686a7762021-12-02T16:06:40ZGlobal gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosa10.1038/s41598-017-01982-z2045-2322https://doaj.org/article/7177982607b944ec803bc442d686a7762017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01982-zhttps://doaj.org/toc/2045-2322Abstract The anuran Rhinella spinulosa is distributed along the Andes Range at altitudes that undergo wide daily and seasonal variation in temperature. One of the populations inhabits geothermal streams, a stable environment that influences life history traits such as the timing of metamorphosis. To investigate whether this population has undergone local adaptation to this unique habitat, we carried out transcriptome analyses in animals from two localities in two developmental stages (prometamorphic and metamorphic) and exposed them to two temperatures (20 and 25 °C). RNA-Seq, de novo assembly and annotation defined a transcriptome revealing 194,469 high quality SNPs, with 1,507 genes under positive selection. Comparisons among the experimental conditions yielded 1,593 differentially expressed genes. A bioinformatics search for candidates revealed a total of 70 genes that are highly likely to be implicated in the adaptive response of the population living in a stable environment, compared to those living in an environment with variable temperatures. Most importantly, the population inhabiting the geothermal environment showed decreased transcriptional plasticity and reduced genetic variation compared to its counterpart from the non-stable environment. This analysis will help to advance the understanding of the molecular mechanisms that account for the local adaptation to geothermal streams in anurans.Luis PastenesCamilo ValdiviesoAlex Di GenovaDante TravisanyAndrew HartMartín MontecinoAriel OrellanaMauricio GonzalezRodrigo A. GutiérrezMiguel L. AllendeAlejandro MaassMarco A. MéndezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luis Pastenes Camilo Valdivieso Alex Di Genova Dante Travisany Andrew Hart Martín Montecino Ariel Orellana Mauricio Gonzalez Rodrigo A. Gutiérrez Miguel L. Allende Alejandro Maass Marco A. Méndez Global gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosa
description	Abstract The anuran Rhinella spinulosa is distributed along the Andes Range at altitudes that undergo wide daily and seasonal variation in temperature. One of the populations inhabits geothermal streams, a stable environment that influences life history traits such as the timing of metamorphosis. To investigate whether this population has undergone local adaptation to this unique habitat, we carried out transcriptome analyses in animals from two localities in two developmental stages (prometamorphic and metamorphic) and exposed them to two temperatures (20 and 25 °C). RNA-Seq, de novo assembly and annotation defined a transcriptome revealing 194,469 high quality SNPs, with 1,507 genes under positive selection. Comparisons among the experimental conditions yielded 1,593 differentially expressed genes. A bioinformatics search for candidates revealed a total of 70 genes that are highly likely to be implicated in the adaptive response of the population living in a stable environment, compared to those living in an environment with variable temperatures. Most importantly, the population inhabiting the geothermal environment showed decreased transcriptional plasticity and reduced genetic variation compared to its counterpart from the non-stable environment. This analysis will help to advance the understanding of the molecular mechanisms that account for the local adaptation to geothermal streams in anurans.
format	article
author	Luis Pastenes Camilo Valdivieso Alex Di Genova Dante Travisany Andrew Hart Martín Montecino Ariel Orellana Mauricio Gonzalez Rodrigo A. Gutiérrez Miguel L. Allende Alejandro Maass Marco A. Méndez
author_facet	Luis Pastenes Camilo Valdivieso Alex Di Genova Dante Travisany Andrew Hart Martín Montecino Ariel Orellana Mauricio Gonzalez Rodrigo A. Gutiérrez Miguel L. Allende Alejandro Maass Marco A. Méndez
author_sort	Luis Pastenes
title	Global gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosa
title_short	Global gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosa
title_full	Global gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosa
title_fullStr	Global gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosa
title_full_unstemmed	Global gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosa
title_sort	global gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the andean toad rhinella spinulosa
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7177982607b944ec803bc442d686a776
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Global gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosa

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