Molecular mechanism of lateral bud differentiation of Pinus massoniana based on high-throughput sequencing

Abstract Knot-free timber cultivation is an important goal of forest breeding, and lateral shoots affect yield and stem shape of tree. The purpose of this study was to analyze the molecular mechanism of lateral bud development by removing the apical dominance of Pinus massoniana young seedlings thro...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hu Chen, Jianhui Tan, Xingxing Liang, Shengsen Tang, Jie Jia, Zhangqi Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/717db88f128f4d97955130b67ce50d62
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:717db88f128f4d97955130b67ce50d62
record_format	dspace
spelling	oai:doaj.org-article:717db88f128f4d97955130b67ce50d622021-12-02T14:53:43ZMolecular mechanism of lateral bud differentiation of Pinus massoniana based on high-throughput sequencing10.1038/s41598-021-87787-72045-2322https://doaj.org/article/717db88f128f4d97955130b67ce50d622021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87787-7https://doaj.org/toc/2045-2322Abstract Knot-free timber cultivation is an important goal of forest breeding, and lateral shoots affect yield and stem shape of tree. The purpose of this study was to analyze the molecular mechanism of lateral bud development by removing the apical dominance of Pinus massoniana young seedlings through transcriptome sequencing and identify key genes involved in lateral bud development. We analyzed hormone contents and transcriptome data for removal of apical dominant of lateral buds as well as apical and lateral buds of normal development ones. Data were analyzed using an comprehensive approach of pathway- and gene-set enrichment analysis, Mapman visualization tool, and gene expression analysis. Our results showed that the contents of auxin (IAA), Zea and strigolactone (SL) in lateral buds significantly increased after removal of apical dominance, while abscisic acid (ABA) decreased. Gibberellin (GA) metabolism, cytokinin (CK), jasmonic acid, zeatin pathway-related genes positively regulated lateral bud development, ABA metabolism-related genes basically negatively regulated lateral bud differentiation, auxin, ethylene, SLs were positive and negative regulation, while only A small number of genes of SA and BRASSINOSTEROID, such as TGA and TCH4, were involved in lateral bud development. In addition, it was speculated that transcription factors such as WRKY, TCP, MYB, HSP, AuxIAA, and AP2 played important roles in the development of lateral buds. In summary, our results provided a better understanding of lateral bud differentiation and lateral shoot formation of P. massoniana from transcriptome level. It provided a basis for molecular characteristics of side branch formation of other timber forests, and contributed to knot-free breeding of forest trees.Hu ChenJianhui TanXingxing LiangShengsen TangJie JiaZhangqi YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hu Chen Jianhui Tan Xingxing Liang Shengsen Tang Jie Jia Zhangqi Yang Molecular mechanism of lateral bud differentiation of Pinus massoniana based on high-throughput sequencing
description	Abstract Knot-free timber cultivation is an important goal of forest breeding, and lateral shoots affect yield and stem shape of tree. The purpose of this study was to analyze the molecular mechanism of lateral bud development by removing the apical dominance of Pinus massoniana young seedlings through transcriptome sequencing and identify key genes involved in lateral bud development. We analyzed hormone contents and transcriptome data for removal of apical dominant of lateral buds as well as apical and lateral buds of normal development ones. Data were analyzed using an comprehensive approach of pathway- and gene-set enrichment analysis, Mapman visualization tool, and gene expression analysis. Our results showed that the contents of auxin (IAA), Zea and strigolactone (SL) in lateral buds significantly increased after removal of apical dominance, while abscisic acid (ABA) decreased. Gibberellin (GA) metabolism, cytokinin (CK), jasmonic acid, zeatin pathway-related genes positively regulated lateral bud development, ABA metabolism-related genes basically negatively regulated lateral bud differentiation, auxin, ethylene, SLs were positive and negative regulation, while only A small number of genes of SA and BRASSINOSTEROID, such as TGA and TCH4, were involved in lateral bud development. In addition, it was speculated that transcription factors such as WRKY, TCP, MYB, HSP, AuxIAA, and AP2 played important roles in the development of lateral buds. In summary, our results provided a better understanding of lateral bud differentiation and lateral shoot formation of P. massoniana from transcriptome level. It provided a basis for molecular characteristics of side branch formation of other timber forests, and contributed to knot-free breeding of forest trees.
format	article
author	Hu Chen Jianhui Tan Xingxing Liang Shengsen Tang Jie Jia Zhangqi Yang
author_facet	Hu Chen Jianhui Tan Xingxing Liang Shengsen Tang Jie Jia Zhangqi Yang
author_sort	Hu Chen
title	Molecular mechanism of lateral bud differentiation of Pinus massoniana based on high-throughput sequencing
title_short	Molecular mechanism of lateral bud differentiation of Pinus massoniana based on high-throughput sequencing
title_full	Molecular mechanism of lateral bud differentiation of Pinus massoniana based on high-throughput sequencing
title_fullStr	Molecular mechanism of lateral bud differentiation of Pinus massoniana based on high-throughput sequencing
title_full_unstemmed	Molecular mechanism of lateral bud differentiation of Pinus massoniana based on high-throughput sequencing
title_sort	molecular mechanism of lateral bud differentiation of pinus massoniana based on high-throughput sequencing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/717db88f128f4d97955130b67ce50d62
work_keys_str_mv	AT huchen molecularmechanismoflateralbuddifferentiationofpinusmassonianabasedonhighthroughputsequencing AT jianhuitan molecularmechanismoflateralbuddifferentiationofpinusmassonianabasedonhighthroughputsequencing AT xingxingliang molecularmechanismoflateralbuddifferentiationofpinusmassonianabasedonhighthroughputsequencing AT shengsentang molecularmechanismoflateralbuddifferentiationofpinusmassonianabasedonhighthroughputsequencing AT jiejia molecularmechanismoflateralbuddifferentiationofpinusmassonianabasedonhighthroughputsequencing AT zhangqiyang molecularmechanismoflateralbuddifferentiationofpinusmassonianabasedonhighthroughputsequencing
_version_	1718389390568325120

Molecular mechanism of lateral bud differentiation of Pinus massoniana based on high-throughput sequencing

Ejemplares similares