Metabolic rewiring is associated with HPV-specific profiles in cervical cancer cell lines

Abstract Both HPV-positive and HPV-negative cervical cancers are associated with aberrant metabolism, although the oncogenic drivers remain elusive. Here we show the assessment of the metabolomic profiles of four distinct cervical cell lines, a normal and three cancer cell lines, one HPV-negative (C...

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Autores principales:	Kalliopi I. Pappa, George Daskalakis, Nicholas P. Anagnou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/717e866d8941493791dbefb1260661d8
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spelling	oai:doaj.org-article:717e866d8941493791dbefb1260661d82021-12-02T17:19:14ZMetabolic rewiring is associated with HPV-specific profiles in cervical cancer cell lines10.1038/s41598-021-96038-82045-2322https://doaj.org/article/717e866d8941493791dbefb1260661d82021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96038-8https://doaj.org/toc/2045-2322Abstract Both HPV-positive and HPV-negative cervical cancers are associated with aberrant metabolism, although the oncogenic drivers remain elusive. Here we show the assessment of the metabolomic profiles of four distinct cervical cell lines, a normal and three cancer cell lines, one HPV-negative (C33A) and two HPV-positive (SiHa HPV16+, HeLa HPV18+), employing an ultra performance liquid chromatography and a high resolution mass spectrometry. Out of the total 462 metabolites, 248 to 326 exhibited statistically significant differences, while Random Forests analysis identified unique molecules for each cell line. The two HPV+ cell lines exhibited features of Warburg metabolism, consistent with the role of the HPV E6 protein. SiHa and HeLa cells displayed purine salvage pathway activity, while C33A cells revealed synthesis of cytidine, via a novel mechanism. These data document a highly dynamic HPV-specific rewiring of metabolic pathways occurring in cervical cancer. Therefore, this approach can eventually provide novel mechanistic insights into cervical carcinogenesis.Kalliopi I. PappaGeorge DaskalakisNicholas P. AnagnouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kalliopi I. Pappa George Daskalakis Nicholas P. Anagnou Metabolic rewiring is associated with HPV-specific profiles in cervical cancer cell lines
description	Abstract Both HPV-positive and HPV-negative cervical cancers are associated with aberrant metabolism, although the oncogenic drivers remain elusive. Here we show the assessment of the metabolomic profiles of four distinct cervical cell lines, a normal and three cancer cell lines, one HPV-negative (C33A) and two HPV-positive (SiHa HPV16+, HeLa HPV18+), employing an ultra performance liquid chromatography and a high resolution mass spectrometry. Out of the total 462 metabolites, 248 to 326 exhibited statistically significant differences, while Random Forests analysis identified unique molecules for each cell line. The two HPV+ cell lines exhibited features of Warburg metabolism, consistent with the role of the HPV E6 protein. SiHa and HeLa cells displayed purine salvage pathway activity, while C33A cells revealed synthesis of cytidine, via a novel mechanism. These data document a highly dynamic HPV-specific rewiring of metabolic pathways occurring in cervical cancer. Therefore, this approach can eventually provide novel mechanistic insights into cervical carcinogenesis.
format	article
author	Kalliopi I. Pappa George Daskalakis Nicholas P. Anagnou
author_facet	Kalliopi I. Pappa George Daskalakis Nicholas P. Anagnou
author_sort	Kalliopi I. Pappa
title	Metabolic rewiring is associated with HPV-specific profiles in cervical cancer cell lines
title_short	Metabolic rewiring is associated with HPV-specific profiles in cervical cancer cell lines
title_full	Metabolic rewiring is associated with HPV-specific profiles in cervical cancer cell lines
title_fullStr	Metabolic rewiring is associated with HPV-specific profiles in cervical cancer cell lines
title_full_unstemmed	Metabolic rewiring is associated with HPV-specific profiles in cervical cancer cell lines
title_sort	metabolic rewiring is associated with hpv-specific profiles in cervical cancer cell lines
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/717e866d8941493791dbefb1260661d8
work_keys_str_mv	AT kalliopiipappa metabolicrewiringisassociatedwithhpvspecificprofilesincervicalcancercelllines AT georgedaskalakis metabolicrewiringisassociatedwithhpvspecificprofilesincervicalcancercelllines AT nicholaspanagnou metabolicrewiringisassociatedwithhpvspecificprofilesincervicalcancercelllines
_version_	1718381092306681856

Metabolic rewiring is associated with HPV-specific profiles in cervical cancer cell lines

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