Oral Microbiome Alterations Associated with Early Childhood Caries Highlight the Importance of Carbohydrate Metabolic Activities
ABSTRACT Globally, dental caries is the most prevalent chronic oral disease and affects roughly half of all children. The aim of this report was to use metagenomic analyses to investigate the relationship between the oral microbiome and caries in preschool children. A total of 25 preschoolers, aged...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
American Society for Microbiology
2019
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/7184df723a3446a1b9f6b83235da5a8c |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:7184df723a3446a1b9f6b83235da5a8c |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:7184df723a3446a1b9f6b83235da5a8c2021-12-02T18:39:16ZOral Microbiome Alterations Associated with Early Childhood Caries Highlight the Importance of Carbohydrate Metabolic Activities10.1128/mSystems.00450-192379-5077https://doaj.org/article/7184df723a3446a1b9f6b83235da5a8c2019-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00450-19https://doaj.org/toc/2379-5077ABSTRACT Globally, dental caries is the most prevalent chronic oral disease and affects roughly half of all children. The aim of this report was to use metagenomic analyses to investigate the relationship between the oral microbiome and caries in preschool children. A total of 25 preschoolers, aged 3 to 5 years old with severe early childhood caries (ECC), and 19 age-matched, caries-free children as controls were recruited. Saliva samples were collected from the participants and were subjected to metagenomic analyses, whereby the oral microbial communities were investigated. The metagenomic analyses revealed substantial microbiota differences between the two groups, indicating apparent shifts of the oral microbiome present in the ECC group. At the species level, the ECC-enriched microbes included Prevotella amnii, Shuttleworthia satelles, Olsenella uli, and Anaeroglobus geminatus. Interestingly, Actinomyces odontolyticus and Actinomyces graevenitzii exhibited apparent differences at the strain level but not the species level between the ECC and control groups. Functional examination showed that the ECC group displayed extensive alterations in metabolic genes/pathways/modules, including enriched functions in sugar metabolism. Finally, an SVM (support vector machine) classifier comprising seven species was developed and generated a moderately good performance in predicting caries onset (area under the receiver operating characteristic curve [AUC] = 78.33%). Together, these findings indicate that caries is associated with considerable changes in the oral microbiome, some of which can potentially be exploited as therapeutic targets or diagnostic markers. (This study has been registered at ClinicalTrials.gov under registration no. NCT02341352.) IMPORTANCE Dental caries is a highly prevalent oral disease that can lead to severe dental damage and may greatly compromise the quality of life of the affected individuals. Previous studies, including those based on 16S rRNA gene, have revealed that the oral microbiota plays a prominent role in development of the disease. But the approach of those studies was limited in analyzing several key microbiome traits, including species- or strain-level composition and functional profile. Here, we performed metagenomic analyses for a cohort of preschool children with or without caries. Our results showed that caries was associated with extensive microbiota differences at various taxonomic and functional levels. Some caries-associated species had not been previously reported, some of which may have significant clinical implications. A microbiome gene catalogue from children with caries was constructed for the first time. The results demonstrated that caries is associated with alterations of the oral microbiome, including changes in microbial composition and metabolic functional profile.Yuan WangSa WangChunyan WuXi ChenZhuhui DuanQian XuWen JiangLei XuTingting WangLingkai SuYing WangYadong ChenJie ZhangYun HuangSuman TongCheng ZhouShuli DengNan QinAmerican Society for Microbiologyarticleearly childhood cariesmetagenomicsoral microbiomefunctional profilepreschool childrenMicrobiologyQR1-502ENmSystems, Vol 4, Iss 6 (2019) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
early childhood caries metagenomics oral microbiome functional profile preschool children Microbiology QR1-502 |
| spellingShingle |
early childhood caries metagenomics oral microbiome functional profile preschool children Microbiology QR1-502 Yuan Wang Sa Wang Chunyan Wu Xi Chen Zhuhui Duan Qian Xu Wen Jiang Lei Xu Tingting Wang Lingkai Su Ying Wang Yadong Chen Jie Zhang Yun Huang Suman Tong Cheng Zhou Shuli Deng Nan Qin Oral Microbiome Alterations Associated with Early Childhood Caries Highlight the Importance of Carbohydrate Metabolic Activities |
| description |
ABSTRACT Globally, dental caries is the most prevalent chronic oral disease and affects roughly half of all children. The aim of this report was to use metagenomic analyses to investigate the relationship between the oral microbiome and caries in preschool children. A total of 25 preschoolers, aged 3 to 5 years old with severe early childhood caries (ECC), and 19 age-matched, caries-free children as controls were recruited. Saliva samples were collected from the participants and were subjected to metagenomic analyses, whereby the oral microbial communities were investigated. The metagenomic analyses revealed substantial microbiota differences between the two groups, indicating apparent shifts of the oral microbiome present in the ECC group. At the species level, the ECC-enriched microbes included Prevotella amnii, Shuttleworthia satelles, Olsenella uli, and Anaeroglobus geminatus. Interestingly, Actinomyces odontolyticus and Actinomyces graevenitzii exhibited apparent differences at the strain level but not the species level between the ECC and control groups. Functional examination showed that the ECC group displayed extensive alterations in metabolic genes/pathways/modules, including enriched functions in sugar metabolism. Finally, an SVM (support vector machine) classifier comprising seven species was developed and generated a moderately good performance in predicting caries onset (area under the receiver operating characteristic curve [AUC] = 78.33%). Together, these findings indicate that caries is associated with considerable changes in the oral microbiome, some of which can potentially be exploited as therapeutic targets or diagnostic markers. (This study has been registered at ClinicalTrials.gov under registration no. NCT02341352.) IMPORTANCE Dental caries is a highly prevalent oral disease that can lead to severe dental damage and may greatly compromise the quality of life of the affected individuals. Previous studies, including those based on 16S rRNA gene, have revealed that the oral microbiota plays a prominent role in development of the disease. But the approach of those studies was limited in analyzing several key microbiome traits, including species- or strain-level composition and functional profile. Here, we performed metagenomic analyses for a cohort of preschool children with or without caries. Our results showed that caries was associated with extensive microbiota differences at various taxonomic and functional levels. Some caries-associated species had not been previously reported, some of which may have significant clinical implications. A microbiome gene catalogue from children with caries was constructed for the first time. The results demonstrated that caries is associated with alterations of the oral microbiome, including changes in microbial composition and metabolic functional profile. |
| format |
article |
| author |
Yuan Wang Sa Wang Chunyan Wu Xi Chen Zhuhui Duan Qian Xu Wen Jiang Lei Xu Tingting Wang Lingkai Su Ying Wang Yadong Chen Jie Zhang Yun Huang Suman Tong Cheng Zhou Shuli Deng Nan Qin |
| author_facet |
Yuan Wang Sa Wang Chunyan Wu Xi Chen Zhuhui Duan Qian Xu Wen Jiang Lei Xu Tingting Wang Lingkai Su Ying Wang Yadong Chen Jie Zhang Yun Huang Suman Tong Cheng Zhou Shuli Deng Nan Qin |
| author_sort |
Yuan Wang |
| title |
Oral Microbiome Alterations Associated with Early Childhood Caries Highlight the Importance of Carbohydrate Metabolic Activities |
| title_short |
Oral Microbiome Alterations Associated with Early Childhood Caries Highlight the Importance of Carbohydrate Metabolic Activities |
| title_full |
Oral Microbiome Alterations Associated with Early Childhood Caries Highlight the Importance of Carbohydrate Metabolic Activities |
| title_fullStr |
Oral Microbiome Alterations Associated with Early Childhood Caries Highlight the Importance of Carbohydrate Metabolic Activities |
| title_full_unstemmed |
Oral Microbiome Alterations Associated with Early Childhood Caries Highlight the Importance of Carbohydrate Metabolic Activities |
| title_sort |
oral microbiome alterations associated with early childhood caries highlight the importance of carbohydrate metabolic activities |
| publisher |
American Society for Microbiology |
| publishDate |
2019 |
| url |
https://doaj.org/article/7184df723a3446a1b9f6b83235da5a8c |
| work_keys_str_mv |
AT yuanwang oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT sawang oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT chunyanwu oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT xichen oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT zhuhuiduan oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT qianxu oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT wenjiang oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT leixu oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT tingtingwang oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT lingkaisu oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT yingwang oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT yadongchen oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT jiezhang oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT yunhuang oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT sumantong oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT chengzhou oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT shulideng oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities AT nanqin oralmicrobiomealterationsassociatedwithearlychildhoodcarieshighlighttheimportanceofcarbohydratemetabolicactivities |
| _version_ |
1718377744275865600 |