Tryptophan-derived metabolites and BAK1 separately contribute to Arabidopsis postinvasive immunity against Alternaria brassicicola

Abstract Nonhost resistance of Arabidopsis thaliana against the hemibiotrophic fungus Colletotrichum tropicale requires PEN2-dependent preinvasive resistance and CYP71A12 and CYP71A13-dependent postinvasive resistance, which both rely on tryptophan (Trp) metabolism. We here revealed that CYP71A12, C...

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Autores principales:	Ayumi Kosaka, Marta Pastorczyk, Mariola Piślewska-Bednarek, Takumi Nishiuchi, Erika Ono, Haruka Suemoto, Atsushi Ishikawa, Henning Frerigmann, Masanori Kaido, Kazuyuki Mise, Paweł Bednarek, Yoshitaka Takano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7185c909e24c436b889b20840639238c
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spelling	oai:doaj.org-article:7185c909e24c436b889b20840639238c2021-12-02T14:12:08ZTryptophan-derived metabolites and BAK1 separately contribute to Arabidopsis postinvasive immunity against Alternaria brassicicola10.1038/s41598-020-79562-x2045-2322https://doaj.org/article/7185c909e24c436b889b20840639238c2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79562-xhttps://doaj.org/toc/2045-2322Abstract Nonhost resistance of Arabidopsis thaliana against the hemibiotrophic fungus Colletotrichum tropicale requires PEN2-dependent preinvasive resistance and CYP71A12 and CYP71A13-dependent postinvasive resistance, which both rely on tryptophan (Trp) metabolism. We here revealed that CYP71A12, CYP71A13 and PAD3 are critical for Arabidopsis’ postinvasive basal resistance toward the necrotrophic Alternaria brassicicola. Consistent with this, gene expression and metabolite analyses suggested that the invasion by A. brassicicola triggered the CYP71A12-dependent production of indole-3-carboxylic acid derivatives and the PAD3 and CYP71A13-dependent production of camalexin. We next addressed the activation of the CYP71A12 and PAD3-dependent postinvasive resistance. We found that bak1-5 mutation significantly reduced postinvasive resistance against A. brassicicola, indicating that pattern recognition contributes to activation of this second defense-layer. However, the bak1-5 mutation had no detectable effects on the Trp-metabolism triggered by the fungal penetration. Together with this, further comparative gene expression analyses suggested that pathogen invasion in Arabidopsis activates (1) CYP71A12 and PAD3-related antifungal metabolism that is not hampered by bak1-5, and (2) a bak1-5 sensitive immune pathway that activates the expression of antimicrobial proteins.Ayumi KosakaMarta PastorczykMariola Piślewska-BednarekTakumi NishiuchiErika OnoHaruka SuemotoAtsushi IshikawaHenning FrerigmannMasanori KaidoKazuyuki MisePaweł BednarekYoshitaka TakanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ayumi Kosaka Marta Pastorczyk Mariola Piślewska-Bednarek Takumi Nishiuchi Erika Ono Haruka Suemoto Atsushi Ishikawa Henning Frerigmann Masanori Kaido Kazuyuki Mise Paweł Bednarek Yoshitaka Takano Tryptophan-derived metabolites and BAK1 separately contribute to Arabidopsis postinvasive immunity against Alternaria brassicicola
description	Abstract Nonhost resistance of Arabidopsis thaliana against the hemibiotrophic fungus Colletotrichum tropicale requires PEN2-dependent preinvasive resistance and CYP71A12 and CYP71A13-dependent postinvasive resistance, which both rely on tryptophan (Trp) metabolism. We here revealed that CYP71A12, CYP71A13 and PAD3 are critical for Arabidopsis’ postinvasive basal resistance toward the necrotrophic Alternaria brassicicola. Consistent with this, gene expression and metabolite analyses suggested that the invasion by A. brassicicola triggered the CYP71A12-dependent production of indole-3-carboxylic acid derivatives and the PAD3 and CYP71A13-dependent production of camalexin. We next addressed the activation of the CYP71A12 and PAD3-dependent postinvasive resistance. We found that bak1-5 mutation significantly reduced postinvasive resistance against A. brassicicola, indicating that pattern recognition contributes to activation of this second defense-layer. However, the bak1-5 mutation had no detectable effects on the Trp-metabolism triggered by the fungal penetration. Together with this, further comparative gene expression analyses suggested that pathogen invasion in Arabidopsis activates (1) CYP71A12 and PAD3-related antifungal metabolism that is not hampered by bak1-5, and (2) a bak1-5 sensitive immune pathway that activates the expression of antimicrobial proteins.
format	article
author	Ayumi Kosaka Marta Pastorczyk Mariola Piślewska-Bednarek Takumi Nishiuchi Erika Ono Haruka Suemoto Atsushi Ishikawa Henning Frerigmann Masanori Kaido Kazuyuki Mise Paweł Bednarek Yoshitaka Takano
author_facet	Ayumi Kosaka Marta Pastorczyk Mariola Piślewska-Bednarek Takumi Nishiuchi Erika Ono Haruka Suemoto Atsushi Ishikawa Henning Frerigmann Masanori Kaido Kazuyuki Mise Paweł Bednarek Yoshitaka Takano
author_sort	Ayumi Kosaka
title	Tryptophan-derived metabolites and BAK1 separately contribute to Arabidopsis postinvasive immunity against Alternaria brassicicola
title_short	Tryptophan-derived metabolites and BAK1 separately contribute to Arabidopsis postinvasive immunity against Alternaria brassicicola
title_full	Tryptophan-derived metabolites and BAK1 separately contribute to Arabidopsis postinvasive immunity against Alternaria brassicicola
title_fullStr	Tryptophan-derived metabolites and BAK1 separately contribute to Arabidopsis postinvasive immunity against Alternaria brassicicola
title_full_unstemmed	Tryptophan-derived metabolites and BAK1 separately contribute to Arabidopsis postinvasive immunity against Alternaria brassicicola
title_sort	tryptophan-derived metabolites and bak1 separately contribute to arabidopsis postinvasive immunity against alternaria brassicicola
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7185c909e24c436b889b20840639238c
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Tryptophan-derived metabolites and BAK1 separately contribute to Arabidopsis postinvasive immunity against Alternaria brassicicola

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