Additive Manufacturing of Caffeic Acid-Inspired Mineral Trioxide Aggregate/Poly-ε-Caprolactone Scaffold for Regulating Vascular Induction and Osteogenic Regeneration of Dental Pulp Stem Cells

Mineral trioxide aggregate (MTA) is a common biomaterial used in endodontics regeneration due to its antibacterial properties, good biocompatibility and high bioactivity. Surface modification technology allows us to endow biomaterials with the necessary biological targets for activation of specific...

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Autores principales:	Ni Tien, Jian-Jr Lee, Alvin Kai-Xing Lee, Yen-Hong Lin, Jian-Xun Chen, Ting-You Kuo, Ming-You Shie
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	angiogenesis caffeic acid human dental pulp stem cell osteogenesis surface modification Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7186ac3cd706485cb384c6b07939f7f8
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spelling	oai:doaj.org-article:7186ac3cd706485cb384c6b07939f7f82021-11-25T17:08:49ZAdditive Manufacturing of Caffeic Acid-Inspired Mineral Trioxide Aggregate/Poly-ε-Caprolactone Scaffold for Regulating Vascular Induction and Osteogenic Regeneration of Dental Pulp Stem Cells10.3390/cells101129112073-4409https://doaj.org/article/7186ac3cd706485cb384c6b07939f7f82021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2911https://doaj.org/toc/2073-4409Mineral trioxide aggregate (MTA) is a common biomaterial used in endodontics regeneration due to its antibacterial properties, good biocompatibility and high bioactivity. Surface modification technology allows us to endow biomaterials with the necessary biological targets for activation of specific downstream functions such as promoting angiogenesis and osteogenesis. In this study, we used caffeic acid (CA)-coated MTA/polycaprolactone (PCL) composites and fabricated 3D scaffolds to evaluate the influence on the physicochemical and biological aspects of CA-coated MTA scaffolds. As seen from the results, modification of CA does not change the original structural characteristics of MTA, thus allowing us to retain the properties of MTA. CA-coated MTA scaffolds were shown to have 25% to 55% higher results than bare scaffold. In addition, CA-coated MTA scaffolds were able to significantly adsorb more vascular endothelial growth factors (<i>p</i> < 0.05) secreted from human dental pulp stem cells (hDPSCs). More importantly, CA-coated MTA scaffolds not only promoted the adhesion and proliferation behaviors of hDPSCs, but also enhanced angiogenesis and osteogenesis. Finally, CA-coated MTA scaffolds led to enhanced subsequent in vivo bone regeneration of the femur of rabbits, which was confirmed using micro-computed tomography and histological staining. Taken together, CA can be used as a potently functional bioactive coating for various scaffolds in bone tissue engineering and other biomedical applications in the future.Ni TienJian-Jr LeeAlvin Kai-Xing LeeYen-Hong LinJian-Xun ChenTing-You KuoMing-You ShieMDPI AGarticleangiogenesiscaffeic acidhuman dental pulp stem cellosteogenesissurface modificationBiology (General)QH301-705.5ENCells, Vol 10, Iss 2911, p 2911 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	angiogenesis caffeic acid human dental pulp stem cell osteogenesis surface modification Biology (General) QH301-705.5
spellingShingle	angiogenesis caffeic acid human dental pulp stem cell osteogenesis surface modification Biology (General) QH301-705.5 Ni Tien Jian-Jr Lee Alvin Kai-Xing Lee Yen-Hong Lin Jian-Xun Chen Ting-You Kuo Ming-You Shie Additive Manufacturing of Caffeic Acid-Inspired Mineral Trioxide Aggregate/Poly-ε-Caprolactone Scaffold for Regulating Vascular Induction and Osteogenic Regeneration of Dental Pulp Stem Cells
description	Mineral trioxide aggregate (MTA) is a common biomaterial used in endodontics regeneration due to its antibacterial properties, good biocompatibility and high bioactivity. Surface modification technology allows us to endow biomaterials with the necessary biological targets for activation of specific downstream functions such as promoting angiogenesis and osteogenesis. In this study, we used caffeic acid (CA)-coated MTA/polycaprolactone (PCL) composites and fabricated 3D scaffolds to evaluate the influence on the physicochemical and biological aspects of CA-coated MTA scaffolds. As seen from the results, modification of CA does not change the original structural characteristics of MTA, thus allowing us to retain the properties of MTA. CA-coated MTA scaffolds were shown to have 25% to 55% higher results than bare scaffold. In addition, CA-coated MTA scaffolds were able to significantly adsorb more vascular endothelial growth factors (<i>p</i> < 0.05) secreted from human dental pulp stem cells (hDPSCs). More importantly, CA-coated MTA scaffolds not only promoted the adhesion and proliferation behaviors of hDPSCs, but also enhanced angiogenesis and osteogenesis. Finally, CA-coated MTA scaffolds led to enhanced subsequent in vivo bone regeneration of the femur of rabbits, which was confirmed using micro-computed tomography and histological staining. Taken together, CA can be used as a potently functional bioactive coating for various scaffolds in bone tissue engineering and other biomedical applications in the future.
format	article
author	Ni Tien Jian-Jr Lee Alvin Kai-Xing Lee Yen-Hong Lin Jian-Xun Chen Ting-You Kuo Ming-You Shie
author_facet	Ni Tien Jian-Jr Lee Alvin Kai-Xing Lee Yen-Hong Lin Jian-Xun Chen Ting-You Kuo Ming-You Shie
author_sort	Ni Tien
title	Additive Manufacturing of Caffeic Acid-Inspired Mineral Trioxide Aggregate/Poly-ε-Caprolactone Scaffold for Regulating Vascular Induction and Osteogenic Regeneration of Dental Pulp Stem Cells
title_short	Additive Manufacturing of Caffeic Acid-Inspired Mineral Trioxide Aggregate/Poly-ε-Caprolactone Scaffold for Regulating Vascular Induction and Osteogenic Regeneration of Dental Pulp Stem Cells
title_full	Additive Manufacturing of Caffeic Acid-Inspired Mineral Trioxide Aggregate/Poly-ε-Caprolactone Scaffold for Regulating Vascular Induction and Osteogenic Regeneration of Dental Pulp Stem Cells
title_fullStr	Additive Manufacturing of Caffeic Acid-Inspired Mineral Trioxide Aggregate/Poly-ε-Caprolactone Scaffold for Regulating Vascular Induction and Osteogenic Regeneration of Dental Pulp Stem Cells
title_full_unstemmed	Additive Manufacturing of Caffeic Acid-Inspired Mineral Trioxide Aggregate/Poly-ε-Caprolactone Scaffold for Regulating Vascular Induction and Osteogenic Regeneration of Dental Pulp Stem Cells
title_sort	additive manufacturing of caffeic acid-inspired mineral trioxide aggregate/poly-ε-caprolactone scaffold for regulating vascular induction and osteogenic regeneration of dental pulp stem cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7186ac3cd706485cb384c6b07939f7f8
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Additive Manufacturing of Caffeic Acid-Inspired Mineral Trioxide Aggregate/Poly-ε-Caprolactone Scaffold for Regulating Vascular Induction and Osteogenic Regeneration of Dental Pulp Stem Cells

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