Genomewide Analysis of Clp1 Function in Transcription in Budding Yeast

Abstract In budding yeast, the 3′ end processing of mRNA and the coupled termination of transcription by RNAPII requires the CF IA complex. We have earlier demonstrated a role for the Clp1 subunit of this complex in termination and promoter-associated transcription of CHA1. To assess the generality...

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Autores principales:	Nadra Al-Husini, Ali Sharifi, Seyed Ahmad Mousavi, Hamidreza Chitsaz, Athar Ansari
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/718c6c8b260044ad9ee751182f043ab0
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spelling	oai:doaj.org-article:718c6c8b260044ad9ee751182f043ab02021-12-02T12:31:52ZGenomewide Analysis of Clp1 Function in Transcription in Budding Yeast10.1038/s41598-017-07062-62045-2322https://doaj.org/article/718c6c8b260044ad9ee751182f043ab02017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07062-6https://doaj.org/toc/2045-2322Abstract In budding yeast, the 3′ end processing of mRNA and the coupled termination of transcription by RNAPII requires the CF IA complex. We have earlier demonstrated a role for the Clp1 subunit of this complex in termination and promoter-associated transcription of CHA1. To assess the generality of the observed function of Clp1 in transcription, we tested the effect of Clp1 on transcription on a genomewide scale using the Global Run-On-Seq (GRO-Seq) approach. GRO-Seq analysis showed the polymerase reading through the termination signal in the downstream region of highly transcribed genes in a temperature-sensitive mutant of Clp1 at elevated temperature. No such terminator readthrough was observed in the mutant at the permissive temperature. The poly(A)-independent termination of transcription of snoRNAs, however, remained unaffected in the absence of Clp1 activity. These results strongly suggest a role for Clp1 in poly(A)-coupled termination of transcription. Furthermore, the density of antisense transcribing polymerase upstream of the promoter region exhibited an increase in the absence of Clp1 activity, thus implicating Clp1 in promoter directionality. The overall conclusion of these results is that Clp1 plays a general role in poly(A)-coupled termination of RNAPII transcription and in enhancing promoter directionality in budding yeast.Nadra Al-HusiniAli SharifiSeyed Ahmad MousaviHamidreza ChitsazAthar AnsariNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nadra Al-Husini Ali Sharifi Seyed Ahmad Mousavi Hamidreza Chitsaz Athar Ansari Genomewide Analysis of Clp1 Function in Transcription in Budding Yeast
description	Abstract In budding yeast, the 3′ end processing of mRNA and the coupled termination of transcription by RNAPII requires the CF IA complex. We have earlier demonstrated a role for the Clp1 subunit of this complex in termination and promoter-associated transcription of CHA1. To assess the generality of the observed function of Clp1 in transcription, we tested the effect of Clp1 on transcription on a genomewide scale using the Global Run-On-Seq (GRO-Seq) approach. GRO-Seq analysis showed the polymerase reading through the termination signal in the downstream region of highly transcribed genes in a temperature-sensitive mutant of Clp1 at elevated temperature. No such terminator readthrough was observed in the mutant at the permissive temperature. The poly(A)-independent termination of transcription of snoRNAs, however, remained unaffected in the absence of Clp1 activity. These results strongly suggest a role for Clp1 in poly(A)-coupled termination of transcription. Furthermore, the density of antisense transcribing polymerase upstream of the promoter region exhibited an increase in the absence of Clp1 activity, thus implicating Clp1 in promoter directionality. The overall conclusion of these results is that Clp1 plays a general role in poly(A)-coupled termination of RNAPII transcription and in enhancing promoter directionality in budding yeast.
format	article
author	Nadra Al-Husini Ali Sharifi Seyed Ahmad Mousavi Hamidreza Chitsaz Athar Ansari
author_facet	Nadra Al-Husini Ali Sharifi Seyed Ahmad Mousavi Hamidreza Chitsaz Athar Ansari
author_sort	Nadra Al-Husini
title	Genomewide Analysis of Clp1 Function in Transcription in Budding Yeast
title_short	Genomewide Analysis of Clp1 Function in Transcription in Budding Yeast
title_full	Genomewide Analysis of Clp1 Function in Transcription in Budding Yeast
title_fullStr	Genomewide Analysis of Clp1 Function in Transcription in Budding Yeast
title_full_unstemmed	Genomewide Analysis of Clp1 Function in Transcription in Budding Yeast
title_sort	genomewide analysis of clp1 function in transcription in budding yeast
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/718c6c8b260044ad9ee751182f043ab0
work_keys_str_mv	AT nadraalhusini genomewideanalysisofclp1functionintranscriptioninbuddingyeast AT alisharifi genomewideanalysisofclp1functionintranscriptioninbuddingyeast AT seyedahmadmousavi genomewideanalysisofclp1functionintranscriptioninbuddingyeast AT hamidrezachitsaz genomewideanalysisofclp1functionintranscriptioninbuddingyeast AT atharansari genomewideanalysisofclp1functionintranscriptioninbuddingyeast
_version_	1718394221754318848

Genomewide Analysis of Clp1 Function in Transcription in Budding Yeast

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