Exploring the human plasma proteome for humoral mediators of remote ischemic preconditioning--a word of caution.

Despite major advances in early revascularization techniques, cardiovascular diseases are still the leading cause of death worldwide, and myocardial infarctions contribute heavily to this. Over the past decades, it has become apparent that reperfusion of blood to a previously ischemic area of the he...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Erik Helgeland, Lars Ertesvåg Breivik, Marc Vaudel, Øyvind Sverre Svendsen, Hilde Garberg, Jan Erik Nordrehaug, Frode Steingrimsen Berven, Anne Kristine Jonassen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/718d14b3bc3a49b3b4272f69a6fc6cf9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:718d14b3bc3a49b3b4272f69a6fc6cf9
record_format	dspace
spelling	oai:doaj.org-article:718d14b3bc3a49b3b4272f69a6fc6cf92021-11-25T05:56:31ZExploring the human plasma proteome for humoral mediators of remote ischemic preconditioning--a word of caution.1932-620310.1371/journal.pone.0109279https://doaj.org/article/718d14b3bc3a49b3b4272f69a6fc6cf92014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0109279https://doaj.org/toc/1932-6203Despite major advances in early revascularization techniques, cardiovascular diseases are still the leading cause of death worldwide, and myocardial infarctions contribute heavily to this. Over the past decades, it has become apparent that reperfusion of blood to a previously ischemic area of the heart causes damage in and of itself, and that this ischemia reperfusion induced injury can be reduced by up to 50% by mechanical manipulation of the blood flow to the heart. The recent discovery of remote ischemic preconditioning (RIPC) provides a non-invasive approach of inducing this cardioprotection at a distance. Finding its endogenous mediators and their operative mode is an important step toward increasing the ischemic tolerance. The release of humoral factor(s) upon RIPC was recently demonstrated and several candidate proteins were published as possible mediators of the cardioprotection. Before clinical applicability, these potential biomarkers and their efficiency must be validated, a task made challenging by the large heterogeneity in reported data and results. Here, in an attempt to reproduce and provide more experimental data on these mediators, we conducted an unbiased in-depth analysis of the human plasma proteome before and after RIPC. From the 68 protein markers reported in the literature, only 28 could be mapped to manually reviewed (Swiss-Prot) protein sequences. 23 of them were monitored in our untargeted experiment. However, their significant regulation could not be reproducibly estimated. In fact, among the 394 plasma proteins we accurately quantified, no significant regulation could be confidently and reproducibly assessed. This indicates that it is difficult to both monitor and reproduce published data from experiments exploring for RIPC induced plasma proteomic regulations, and suggests that further work should be directed towards small humoral factors. To simplify this task, we made our proteomic dataset available via ProteomeXchange, where scientists can mine for novel potential targets.Erik HelgelandLars Ertesvåg BreivikMarc VaudelØyvind Sverre SvendsenHilde GarbergJan Erik NordrehaugFrode Steingrimsen BervenAnne Kristine JonassenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e109279 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Erik Helgeland Lars Ertesvåg Breivik Marc Vaudel Øyvind Sverre Svendsen Hilde Garberg Jan Erik Nordrehaug Frode Steingrimsen Berven Anne Kristine Jonassen Exploring the human plasma proteome for humoral mediators of remote ischemic preconditioning--a word of caution.
description	Despite major advances in early revascularization techniques, cardiovascular diseases are still the leading cause of death worldwide, and myocardial infarctions contribute heavily to this. Over the past decades, it has become apparent that reperfusion of blood to a previously ischemic area of the heart causes damage in and of itself, and that this ischemia reperfusion induced injury can be reduced by up to 50% by mechanical manipulation of the blood flow to the heart. The recent discovery of remote ischemic preconditioning (RIPC) provides a non-invasive approach of inducing this cardioprotection at a distance. Finding its endogenous mediators and their operative mode is an important step toward increasing the ischemic tolerance. The release of humoral factor(s) upon RIPC was recently demonstrated and several candidate proteins were published as possible mediators of the cardioprotection. Before clinical applicability, these potential biomarkers and their efficiency must be validated, a task made challenging by the large heterogeneity in reported data and results. Here, in an attempt to reproduce and provide more experimental data on these mediators, we conducted an unbiased in-depth analysis of the human plasma proteome before and after RIPC. From the 68 protein markers reported in the literature, only 28 could be mapped to manually reviewed (Swiss-Prot) protein sequences. 23 of them were monitored in our untargeted experiment. However, their significant regulation could not be reproducibly estimated. In fact, among the 394 plasma proteins we accurately quantified, no significant regulation could be confidently and reproducibly assessed. This indicates that it is difficult to both monitor and reproduce published data from experiments exploring for RIPC induced plasma proteomic regulations, and suggests that further work should be directed towards small humoral factors. To simplify this task, we made our proteomic dataset available via ProteomeXchange, where scientists can mine for novel potential targets.
format	article
author	Erik Helgeland Lars Ertesvåg Breivik Marc Vaudel Øyvind Sverre Svendsen Hilde Garberg Jan Erik Nordrehaug Frode Steingrimsen Berven Anne Kristine Jonassen
author_facet	Erik Helgeland Lars Ertesvåg Breivik Marc Vaudel Øyvind Sverre Svendsen Hilde Garberg Jan Erik Nordrehaug Frode Steingrimsen Berven Anne Kristine Jonassen
author_sort	Erik Helgeland
title	Exploring the human plasma proteome for humoral mediators of remote ischemic preconditioning--a word of caution.
title_short	Exploring the human plasma proteome for humoral mediators of remote ischemic preconditioning--a word of caution.
title_full	Exploring the human plasma proteome for humoral mediators of remote ischemic preconditioning--a word of caution.
title_fullStr	Exploring the human plasma proteome for humoral mediators of remote ischemic preconditioning--a word of caution.
title_full_unstemmed	Exploring the human plasma proteome for humoral mediators of remote ischemic preconditioning--a word of caution.
title_sort	exploring the human plasma proteome for humoral mediators of remote ischemic preconditioning--a word of caution.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/718d14b3bc3a49b3b4272f69a6fc6cf9
work_keys_str_mv	AT erikhelgeland exploringthehumanplasmaproteomeforhumoralmediatorsofremoteischemicpreconditioningawordofcaution AT larsertesvagbreivik exploringthehumanplasmaproteomeforhumoralmediatorsofremoteischemicpreconditioningawordofcaution AT marcvaudel exploringthehumanplasmaproteomeforhumoralmediatorsofremoteischemicpreconditioningawordofcaution AT øyvindsverresvendsen exploringthehumanplasmaproteomeforhumoralmediatorsofremoteischemicpreconditioningawordofcaution AT hildegarberg exploringthehumanplasmaproteomeforhumoralmediatorsofremoteischemicpreconditioningawordofcaution AT janeriknordrehaug exploringthehumanplasmaproteomeforhumoralmediatorsofremoteischemicpreconditioningawordofcaution AT frodesteingrimsenberven exploringthehumanplasmaproteomeforhumoralmediatorsofremoteischemicpreconditioningawordofcaution AT annekristinejonassen exploringthehumanplasmaproteomeforhumoralmediatorsofremoteischemicpreconditioningawordofcaution
_version_	1718414346975969280

Exploring the human plasma proteome for humoral mediators of remote ischemic preconditioning--a word of caution.

Ejemplares similares