Activation of NLRP3 Inflammasome Complexes by Beta-Tricalcium Phosphate Particles and Stimulation of Immune Cell Migration in vivo

Beta-tricalcium phosphate (β-TCP) serves as a bone substitute in clinical practice because it is resorbable, biocompatible, osteointegrative, and osteoconductive. Particles of β-TCP are also inflammatory mediators although the mechanism of this function has not been fully elucidated. Regardless, the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kouji Maruyama, Jin-Yan Cheng, Hidee Ishii, Yu Takahashi, Vincent Zangiacomi, Takatomo Satoh, Tetsuji Hosono, Ken Yamaguchi
Formato:	article
Lenguaje:	EN
Publicado:	Karger Publishers 2021
Materias:	beta-tricalcium phosphate inflammasome dendritic cell macrophage thp-1 cell immunomodulation Medicine R Internal medicine RC31-1245
Acceso en línea:	https://doaj.org/article/71902c7dfc9b445eb4a81acd9fe93fe1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:71902c7dfc9b445eb4a81acd9fe93fe1
record_format	dspace
spelling	oai:doaj.org-article:71902c7dfc9b445eb4a81acd9fe93fe12021-11-04T14:40:31ZActivation of NLRP3 Inflammasome Complexes by Beta-Tricalcium Phosphate Particles and Stimulation of Immune Cell Migration in vivo1662-811X1662-812810.1159/000518953https://doaj.org/article/71902c7dfc9b445eb4a81acd9fe93fe12021-10-01T00:00:00Zhttps://www.karger.com/Article/FullText/518953https://doaj.org/toc/1662-811Xhttps://doaj.org/toc/1662-8128Beta-tricalcium phosphate (β-TCP) serves as a bone substitute in clinical practice because it is resorbable, biocompatible, osteointegrative, and osteoconductive. Particles of β-TCP are also inflammatory mediators although the mechanism of this function has not been fully elucidated. Regardless, the ability of β-TCP to stimulate the immune system might be useful for immunomodulation. The present study aimed to determine the effects of β-TCP particles on NLR family pyrin domain containing 3 (NLRP3) inflammasome complexes. We found that β-TCP activates NLRP3 inflammasomes, and increases interleukin (IL)-1β production in primary cultured mouse dendritic cells (DCs) and macrophages, and human THP-1 cells in caspase-1 dependent manner. In THP-1 cells, β-TCP increased also IL-18 production, and NLRP3 inflammasome activation by β-TCP depended on phagocytosis, potassium efflux, and reactive oxygen species (ROS) generation. We also investigated the effects of β-TCP in wild-type and NLRP3-deficient mice in vivo. Immune cell migration around subcutaneously injected β-TCP particles was reduced in NLRP3-deficient mice. These findings suggest that the effects of β-TCP particles in vivo are at least partly mediated by NLRP3 inflammasome complexes.Kouji MaruyamaJin-Yan ChengHidee IshiiYu TakahashiVincent ZangiacomiTakatomo SatohTetsuji HosonoKen YamaguchiKarger Publishersarticlebeta-tricalcium phosphateinflammasomedendritic cellmacrophagethp-1 cellimmunomodulationMedicineRInternal medicineRC31-1245ENJournal of Innate Immunity, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	beta-tricalcium phosphate inflammasome dendritic cell macrophage thp-1 cell immunomodulation Medicine R Internal medicine RC31-1245
spellingShingle	beta-tricalcium phosphate inflammasome dendritic cell macrophage thp-1 cell immunomodulation Medicine R Internal medicine RC31-1245 Kouji Maruyama Jin-Yan Cheng Hidee Ishii Yu Takahashi Vincent Zangiacomi Takatomo Satoh Tetsuji Hosono Ken Yamaguchi Activation of NLRP3 Inflammasome Complexes by Beta-Tricalcium Phosphate Particles and Stimulation of Immune Cell Migration in vivo
description	Beta-tricalcium phosphate (β-TCP) serves as a bone substitute in clinical practice because it is resorbable, biocompatible, osteointegrative, and osteoconductive. Particles of β-TCP are also inflammatory mediators although the mechanism of this function has not been fully elucidated. Regardless, the ability of β-TCP to stimulate the immune system might be useful for immunomodulation. The present study aimed to determine the effects of β-TCP particles on NLR family pyrin domain containing 3 (NLRP3) inflammasome complexes. We found that β-TCP activates NLRP3 inflammasomes, and increases interleukin (IL)-1β production in primary cultured mouse dendritic cells (DCs) and macrophages, and human THP-1 cells in caspase-1 dependent manner. In THP-1 cells, β-TCP increased also IL-18 production, and NLRP3 inflammasome activation by β-TCP depended on phagocytosis, potassium efflux, and reactive oxygen species (ROS) generation. We also investigated the effects of β-TCP in wild-type and NLRP3-deficient mice in vivo. Immune cell migration around subcutaneously injected β-TCP particles was reduced in NLRP3-deficient mice. These findings suggest that the effects of β-TCP particles in vivo are at least partly mediated by NLRP3 inflammasome complexes.
format	article
author	Kouji Maruyama Jin-Yan Cheng Hidee Ishii Yu Takahashi Vincent Zangiacomi Takatomo Satoh Tetsuji Hosono Ken Yamaguchi
author_facet	Kouji Maruyama Jin-Yan Cheng Hidee Ishii Yu Takahashi Vincent Zangiacomi Takatomo Satoh Tetsuji Hosono Ken Yamaguchi
author_sort	Kouji Maruyama
title	Activation of NLRP3 Inflammasome Complexes by Beta-Tricalcium Phosphate Particles and Stimulation of Immune Cell Migration in vivo
title_short	Activation of NLRP3 Inflammasome Complexes by Beta-Tricalcium Phosphate Particles and Stimulation of Immune Cell Migration in vivo
title_full	Activation of NLRP3 Inflammasome Complexes by Beta-Tricalcium Phosphate Particles and Stimulation of Immune Cell Migration in vivo
title_fullStr	Activation of NLRP3 Inflammasome Complexes by Beta-Tricalcium Phosphate Particles and Stimulation of Immune Cell Migration in vivo
title_full_unstemmed	Activation of NLRP3 Inflammasome Complexes by Beta-Tricalcium Phosphate Particles and Stimulation of Immune Cell Migration in vivo
title_sort	activation of nlrp3 inflammasome complexes by beta-tricalcium phosphate particles and stimulation of immune cell migration in vivo
publisher	Karger Publishers
publishDate	2021
url	https://doaj.org/article/71902c7dfc9b445eb4a81acd9fe93fe1
work_keys_str_mv	AT koujimaruyama activationofnlrp3inflammasomecomplexesbybetatricalciumphosphateparticlesandstimulationofimmunecellmigrationinvivo AT jinyancheng activationofnlrp3inflammasomecomplexesbybetatricalciumphosphateparticlesandstimulationofimmunecellmigrationinvivo AT hideeishii activationofnlrp3inflammasomecomplexesbybetatricalciumphosphateparticlesandstimulationofimmunecellmigrationinvivo AT yutakahashi activationofnlrp3inflammasomecomplexesbybetatricalciumphosphateparticlesandstimulationofimmunecellmigrationinvivo AT vincentzangiacomi activationofnlrp3inflammasomecomplexesbybetatricalciumphosphateparticlesandstimulationofimmunecellmigrationinvivo AT takatomosatoh activationofnlrp3inflammasomecomplexesbybetatricalciumphosphateparticlesandstimulationofimmunecellmigrationinvivo AT tetsujihosono activationofnlrp3inflammasomecomplexesbybetatricalciumphosphateparticlesandstimulationofimmunecellmigrationinvivo AT kenyamaguchi activationofnlrp3inflammasomecomplexesbybetatricalciumphosphateparticlesandstimulationofimmunecellmigrationinvivo
_version_	1718444845382500352

Activation of NLRP3 Inflammasome Complexes by Beta-Tricalcium Phosphate Particles and Stimulation of Immune Cell Migration in vivo

Ejemplares similares