Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid

Extracellular vesicles (EVs), which exist in the follicular fluid of ruminant ovaries, are considered as cargo carriers for the transfer of biomolecules to recipient cells. However, the functions and changes in EVs in antral follicles remain ambiguous. In the present study, we isolated and character...

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Autores principales:	Qiang Ding, Miaohan Jin, Peter Kalds, Chunhua Meng, Huili Wang, Jifeng Zhong, Xiaolong Wang, Yulin Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	extracellular vesicles follicular fluid small RNA sequence miRNAs Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/71920c186e8f4e459d64849c146c2dfd
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spelling	oai:doaj.org-article:71920c186e8f4e459d64849c146c2dfd2021-11-25T16:18:40ZComparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid10.3390/ani111131902076-2615https://doaj.org/article/71920c186e8f4e459d64849c146c2dfd2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3190https://doaj.org/toc/2076-2615Extracellular vesicles (EVs), which exist in the follicular fluid of ruminant ovaries, are considered as cargo carriers for the transfer of biomolecules to recipient cells. However, the functions and changes in EVs in antral follicles remain ambiguous. In the present study, we isolated and characterized EVs from goat follicular fluid by means of differential ultracentrifugation and Western blotting of marker proteins. Bioinformatics tools were used to detect miRNA expression levels in EVs. Different miRNA expression patterns of EVs exist in small to large follicles. Thirteen differentially expressed miRNAs (seven upregulated and six downregulated) were identified and used for analysis. A total of 1948 predicted target genes of 13 miRNAs were mapped to signaling pathways, and three significantly enriched pathways (FoxO, MAPK, and PI3K-AKT signaling pathways) were involved in follicular development, as revealed by KEGG enrichment analysis. Our findings suggest that EVs in follicular fluid play biofunctional roles during follicular development in goats.Qiang DingMiaohan JinPeter KaldsChunhua MengHuili WangJifeng ZhongXiaolong WangYulin ChenMDPI AGarticleextracellular vesiclesfollicular fluidsmall RNA sequencemiRNAsVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3190, p 3190 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	extracellular vesicles follicular fluid small RNA sequence miRNAs Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	extracellular vesicles follicular fluid small RNA sequence miRNAs Veterinary medicine SF600-1100 Zoology QL1-991 Qiang Ding Miaohan Jin Peter Kalds Chunhua Meng Huili Wang Jifeng Zhong Xiaolong Wang Yulin Chen Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid
description	Extracellular vesicles (EVs), which exist in the follicular fluid of ruminant ovaries, are considered as cargo carriers for the transfer of biomolecules to recipient cells. However, the functions and changes in EVs in antral follicles remain ambiguous. In the present study, we isolated and characterized EVs from goat follicular fluid by means of differential ultracentrifugation and Western blotting of marker proteins. Bioinformatics tools were used to detect miRNA expression levels in EVs. Different miRNA expression patterns of EVs exist in small to large follicles. Thirteen differentially expressed miRNAs (seven upregulated and six downregulated) were identified and used for analysis. A total of 1948 predicted target genes of 13 miRNAs were mapped to signaling pathways, and three significantly enriched pathways (FoxO, MAPK, and PI3K-AKT signaling pathways) were involved in follicular development, as revealed by KEGG enrichment analysis. Our findings suggest that EVs in follicular fluid play biofunctional roles during follicular development in goats.
format	article
author	Qiang Ding Miaohan Jin Peter Kalds Chunhua Meng Huili Wang Jifeng Zhong Xiaolong Wang Yulin Chen
author_facet	Qiang Ding Miaohan Jin Peter Kalds Chunhua Meng Huili Wang Jifeng Zhong Xiaolong Wang Yulin Chen
author_sort	Qiang Ding
title	Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid
title_short	Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid
title_full	Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid
title_fullStr	Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid
title_full_unstemmed	Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid
title_sort	comparison of microrna profiles in extracellular vesicles from small and large goat follicular fluid
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/71920c186e8f4e459d64849c146c2dfd
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Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid

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