The interaction of the bioinsecticide PA1b (Pea Albumin 1 subunit b) with the insect V-ATPase triggers apoptosis
Abstract PA1b (Pea Albumin 1, subunit b) peptide is an entomotoxin, extracted from Legume seeds, with a lethal activity towards several insect pests, such as mosquitoes, some aphids and cereal weevils. This toxin acts by binding to the subunits c and e of the plasma membrane H+-ATPase (V-ATPase) in...
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Nature Portfolio
2017
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oai:doaj.org-article:7193b3596f33475aa472de278324cf702021-12-02T15:05:38ZThe interaction of the bioinsecticide PA1b (Pea Albumin 1 subunit b) with the insect V-ATPase triggers apoptosis10.1038/s41598-017-05315-y2045-2322https://doaj.org/article/7193b3596f33475aa472de278324cf702017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05315-yhttps://doaj.org/toc/2045-2322Abstract PA1b (Pea Albumin 1, subunit b) peptide is an entomotoxin, extracted from Legume seeds, with a lethal activity towards several insect pests, such as mosquitoes, some aphids and cereal weevils. This toxin acts by binding to the subunits c and e of the plasma membrane H+-ATPase (V-ATPase) in the insect midgut. In this study, two cereal weevils, the sensitive Sitophilus oryzae strain WAA42, the resistance Sitophilus oryzae strain ISOR3 and the insensitive red flour beetle Tribolium castaneum, were used in biochemical and histological experiments to demonstrate that a PA1b/V-ATPase interaction triggers the apoptosis mechanism, resulting in insect death. Upon intoxication with PA1b, apoptotic bodies are formed in the cells of the insect midgut. In addition, caspase-3 enzyme activity occurs in the midgut of sensitive weevils after intoxication with active PA1b, but not in the midgut of resistant weevils. These biochemical data were confirmed by immuno-histochemical detection of the caspase-3 active form in the midgut of sensitive weevils. Immuno-labelling experiments also revealed that the caspase-3 active form and V-ATPase are close-localized in the insect midgut. The results concerning this unique peptidic V-ATPase inhibitor pave the way for the utilization of PA1b as a promising, more selective and eco-friendly insecticide.Vanessa EyraudSéverine BalmandLamis KarakiIsabelle RahiouiCatherine SivignonAgnès F. DelmasCorinne RoyerYvan RahbéPedro Da SilvaFrédéric GressentNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
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Medicine R Science Q Vanessa Eyraud Séverine Balmand Lamis Karaki Isabelle Rahioui Catherine Sivignon Agnès F. Delmas Corinne Royer Yvan Rahbé Pedro Da Silva Frédéric Gressent The interaction of the bioinsecticide PA1b (Pea Albumin 1 subunit b) with the insect V-ATPase triggers apoptosis |
| description |
Abstract PA1b (Pea Albumin 1, subunit b) peptide is an entomotoxin, extracted from Legume seeds, with a lethal activity towards several insect pests, such as mosquitoes, some aphids and cereal weevils. This toxin acts by binding to the subunits c and e of the plasma membrane H+-ATPase (V-ATPase) in the insect midgut. In this study, two cereal weevils, the sensitive Sitophilus oryzae strain WAA42, the resistance Sitophilus oryzae strain ISOR3 and the insensitive red flour beetle Tribolium castaneum, were used in biochemical and histological experiments to demonstrate that a PA1b/V-ATPase interaction triggers the apoptosis mechanism, resulting in insect death. Upon intoxication with PA1b, apoptotic bodies are formed in the cells of the insect midgut. In addition, caspase-3 enzyme activity occurs in the midgut of sensitive weevils after intoxication with active PA1b, but not in the midgut of resistant weevils. These biochemical data were confirmed by immuno-histochemical detection of the caspase-3 active form in the midgut of sensitive weevils. Immuno-labelling experiments also revealed that the caspase-3 active form and V-ATPase are close-localized in the insect midgut. The results concerning this unique peptidic V-ATPase inhibitor pave the way for the utilization of PA1b as a promising, more selective and eco-friendly insecticide. |
| format |
article |
| author |
Vanessa Eyraud Séverine Balmand Lamis Karaki Isabelle Rahioui Catherine Sivignon Agnès F. Delmas Corinne Royer Yvan Rahbé Pedro Da Silva Frédéric Gressent |
| author_facet |
Vanessa Eyraud Séverine Balmand Lamis Karaki Isabelle Rahioui Catherine Sivignon Agnès F. Delmas Corinne Royer Yvan Rahbé Pedro Da Silva Frédéric Gressent |
| author_sort |
Vanessa Eyraud |
| title |
The interaction of the bioinsecticide PA1b (Pea Albumin 1 subunit b) with the insect V-ATPase triggers apoptosis |
| title_short |
The interaction of the bioinsecticide PA1b (Pea Albumin 1 subunit b) with the insect V-ATPase triggers apoptosis |
| title_full |
The interaction of the bioinsecticide PA1b (Pea Albumin 1 subunit b) with the insect V-ATPase triggers apoptosis |
| title_fullStr |
The interaction of the bioinsecticide PA1b (Pea Albumin 1 subunit b) with the insect V-ATPase triggers apoptosis |
| title_full_unstemmed |
The interaction of the bioinsecticide PA1b (Pea Albumin 1 subunit b) with the insect V-ATPase triggers apoptosis |
| title_sort |
interaction of the bioinsecticide pa1b (pea albumin 1 subunit b) with the insect v-atpase triggers apoptosis |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/7193b3596f33475aa472de278324cf70 |
| work_keys_str_mv |
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