Fucosylated Human Milk Oligosaccharides and N-Glycans in the Milk of Chinese Mothers Regulate the Gut Microbiome of Their Breast-Fed Infants during Different Lactation Stages
ABSTRACT The milk glycobiome has a significant impact on the gut microbiota of infants, which plays a pivotal role in health and development. Fucosylated human milk oligosaccharides (HMOs) and N-glycans on milk proteins are beneficial for the development of healthy gut microbiota, and the fucosylati...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
American Society for Microbiology
2018
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/71945bbf4bd34500aba6cd7b408b8bef |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:71945bbf4bd34500aba6cd7b408b8bef |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:71945bbf4bd34500aba6cd7b408b8bef2021-12-02T19:46:17ZFucosylated Human Milk Oligosaccharides and N-Glycans in the Milk of Chinese Mothers Regulate the Gut Microbiome of Their Breast-Fed Infants during Different Lactation Stages10.1128/mSystems.00206-182379-5077https://doaj.org/article/71945bbf4bd34500aba6cd7b408b8bef2018-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00206-18https://doaj.org/toc/2379-5077ABSTRACT The milk glycobiome has a significant impact on the gut microbiota of infants, which plays a pivotal role in health and development. Fucosylated human milk oligosaccharides (HMOs) and N-glycans on milk proteins are beneficial for the development of healthy gut microbiota, and the fucosylation levels of these glycans can be affected by the maternal fucosyltransferase 2 gene (FUT2). Here, we present results of longitudinal research on paired milk and stool samples from 56 Chinese mothers (CMs) and their breast-fed children. Changes of HMOs and fucosylated N-glycans in milk of CMs at different lactation stages were detected, which allowed characterization of the major differences in milk glycans and consequential effects on the gut microbiome of infants according to maternal FUT2 status. Significant differences in the abundance of total and fucosylated HMOs between secretor and nonsecretor CMs were noted, especially during early lactation. Despite a tendency toward decreasing milk protein concentrations, the fucosylation levels of milk N-glycans increased during late lactation. The changes in the levels of fucosylated HMOs and milk N-glycans were highly correlated with the growth of Bifidobacterium spp. and Lactobacillus spp. in the gut of infants during early and later lactation, respectively. Enriched expression of genes encoding glycoside hydrolases, glycosyl transferases, ATP-binding cassette (ABC) transporters, and permeases in infants fed by secretor CMs contributed to the promotion of these bacteria in infants. Our data highlight the important role of fucosylated milk glycans in shaping the gut microbiome of infants and provide a solid foundation for development of “personalized” nutrition for Chinese infants. IMPORTANCE Human milk glycans provide a broad range of carbon sources for gut microbes in infants. Levels of protein glycosylation in human milk vary during lactation and may also be affected by the stages of gestation and lactation and by the secretor status of the mother. This was the first study to evaluate systematically dynamic changes in human milk oligosaccharides and fucosylated N-glycans in the milk of Chinese mothers with different secretor statuses during 6 months of lactation. Given the unique single nucleotide polymorphism site (rs1047781, A385T) on the fucosyltransferase 2 gene among Chinese populations, our report provides a specific insight into the milk glycobiome of Chinese mothers, which may exert effects on the gut microbiota of infants that differ from findings from other study cohorts.Yaqiang BaiJia TaoJiaorui ZhouQingjie FanMan LiuYuqi HuYao XuLilong ZhangJieli YuanWenzhe LiXiaolei ZePatrice MalardZhimou GuoJingyu YanMing LiAmerican Society for MicrobiologyarticleFUT2HMOsfucosylationgut microbiomemilk N-glycansMicrobiologyQR1-502ENmSystems, Vol 3, Iss 6 (2018) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
FUT2 HMOs fucosylation gut microbiome milk N-glycans Microbiology QR1-502 |
| spellingShingle |
FUT2 HMOs fucosylation gut microbiome milk N-glycans Microbiology QR1-502 Yaqiang Bai Jia Tao Jiaorui Zhou Qingjie Fan Man Liu Yuqi Hu Yao Xu Lilong Zhang Jieli Yuan Wenzhe Li Xiaolei Ze Patrice Malard Zhimou Guo Jingyu Yan Ming Li Fucosylated Human Milk Oligosaccharides and N-Glycans in the Milk of Chinese Mothers Regulate the Gut Microbiome of Their Breast-Fed Infants during Different Lactation Stages |
| description |
ABSTRACT The milk glycobiome has a significant impact on the gut microbiota of infants, which plays a pivotal role in health and development. Fucosylated human milk oligosaccharides (HMOs) and N-glycans on milk proteins are beneficial for the development of healthy gut microbiota, and the fucosylation levels of these glycans can be affected by the maternal fucosyltransferase 2 gene (FUT2). Here, we present results of longitudinal research on paired milk and stool samples from 56 Chinese mothers (CMs) and their breast-fed children. Changes of HMOs and fucosylated N-glycans in milk of CMs at different lactation stages were detected, which allowed characterization of the major differences in milk glycans and consequential effects on the gut microbiome of infants according to maternal FUT2 status. Significant differences in the abundance of total and fucosylated HMOs between secretor and nonsecretor CMs were noted, especially during early lactation. Despite a tendency toward decreasing milk protein concentrations, the fucosylation levels of milk N-glycans increased during late lactation. The changes in the levels of fucosylated HMOs and milk N-glycans were highly correlated with the growth of Bifidobacterium spp. and Lactobacillus spp. in the gut of infants during early and later lactation, respectively. Enriched expression of genes encoding glycoside hydrolases, glycosyl transferases, ATP-binding cassette (ABC) transporters, and permeases in infants fed by secretor CMs contributed to the promotion of these bacteria in infants. Our data highlight the important role of fucosylated milk glycans in shaping the gut microbiome of infants and provide a solid foundation for development of “personalized” nutrition for Chinese infants. IMPORTANCE Human milk glycans provide a broad range of carbon sources for gut microbes in infants. Levels of protein glycosylation in human milk vary during lactation and may also be affected by the stages of gestation and lactation and by the secretor status of the mother. This was the first study to evaluate systematically dynamic changes in human milk oligosaccharides and fucosylated N-glycans in the milk of Chinese mothers with different secretor statuses during 6 months of lactation. Given the unique single nucleotide polymorphism site (rs1047781, A385T) on the fucosyltransferase 2 gene among Chinese populations, our report provides a specific insight into the milk glycobiome of Chinese mothers, which may exert effects on the gut microbiota of infants that differ from findings from other study cohorts. |
| format |
article |
| author |
Yaqiang Bai Jia Tao Jiaorui Zhou Qingjie Fan Man Liu Yuqi Hu Yao Xu Lilong Zhang Jieli Yuan Wenzhe Li Xiaolei Ze Patrice Malard Zhimou Guo Jingyu Yan Ming Li |
| author_facet |
Yaqiang Bai Jia Tao Jiaorui Zhou Qingjie Fan Man Liu Yuqi Hu Yao Xu Lilong Zhang Jieli Yuan Wenzhe Li Xiaolei Ze Patrice Malard Zhimou Guo Jingyu Yan Ming Li |
| author_sort |
Yaqiang Bai |
| title |
Fucosylated Human Milk Oligosaccharides and N-Glycans in the Milk of Chinese Mothers Regulate the Gut Microbiome of Their Breast-Fed Infants during Different Lactation Stages |
| title_short |
Fucosylated Human Milk Oligosaccharides and N-Glycans in the Milk of Chinese Mothers Regulate the Gut Microbiome of Their Breast-Fed Infants during Different Lactation Stages |
| title_full |
Fucosylated Human Milk Oligosaccharides and N-Glycans in the Milk of Chinese Mothers Regulate the Gut Microbiome of Their Breast-Fed Infants during Different Lactation Stages |
| title_fullStr |
Fucosylated Human Milk Oligosaccharides and N-Glycans in the Milk of Chinese Mothers Regulate the Gut Microbiome of Their Breast-Fed Infants during Different Lactation Stages |
| title_full_unstemmed |
Fucosylated Human Milk Oligosaccharides and N-Glycans in the Milk of Chinese Mothers Regulate the Gut Microbiome of Their Breast-Fed Infants during Different Lactation Stages |
| title_sort |
fucosylated human milk oligosaccharides and n-glycans in the milk of chinese mothers regulate the gut microbiome of their breast-fed infants during different lactation stages |
| publisher |
American Society for Microbiology |
| publishDate |
2018 |
| url |
https://doaj.org/article/71945bbf4bd34500aba6cd7b408b8bef |
| work_keys_str_mv |
AT yaqiangbai fucosylatedhumanmilkoligosaccharidesandnglycansinthemilkofchinesemothersregulatethegutmicrobiomeoftheirbreastfedinfantsduringdifferentlactationstages AT jiatao fucosylatedhumanmilkoligosaccharidesandnglycansinthemilkofchinesemothersregulatethegutmicrobiomeoftheirbreastfedinfantsduringdifferentlactationstages AT jiaoruizhou fucosylatedhumanmilkoligosaccharidesandnglycansinthemilkofchinesemothersregulatethegutmicrobiomeoftheirbreastfedinfantsduringdifferentlactationstages AT qingjiefan fucosylatedhumanmilkoligosaccharidesandnglycansinthemilkofchinesemothersregulatethegutmicrobiomeoftheirbreastfedinfantsduringdifferentlactationstages AT manliu fucosylatedhumanmilkoligosaccharidesandnglycansinthemilkofchinesemothersregulatethegutmicrobiomeoftheirbreastfedinfantsduringdifferentlactationstages AT yuqihu fucosylatedhumanmilkoligosaccharidesandnglycansinthemilkofchinesemothersregulatethegutmicrobiomeoftheirbreastfedinfantsduringdifferentlactationstages AT yaoxu fucosylatedhumanmilkoligosaccharidesandnglycansinthemilkofchinesemothersregulatethegutmicrobiomeoftheirbreastfedinfantsduringdifferentlactationstages AT lilongzhang fucosylatedhumanmilkoligosaccharidesandnglycansinthemilkofchinesemothersregulatethegutmicrobiomeoftheirbreastfedinfantsduringdifferentlactationstages AT jieliyuan fucosylatedhumanmilkoligosaccharidesandnglycansinthemilkofchinesemothersregulatethegutmicrobiomeoftheirbreastfedinfantsduringdifferentlactationstages AT wenzheli fucosylatedhumanmilkoligosaccharidesandnglycansinthemilkofchinesemothersregulatethegutmicrobiomeoftheirbreastfedinfantsduringdifferentlactationstages AT xiaoleize fucosylatedhumanmilkoligosaccharidesandnglycansinthemilkofchinesemothersregulatethegutmicrobiomeoftheirbreastfedinfantsduringdifferentlactationstages AT patricemalard fucosylatedhumanmilkoligosaccharidesandnglycansinthemilkofchinesemothersregulatethegutmicrobiomeoftheirbreastfedinfantsduringdifferentlactationstages AT zhimouguo fucosylatedhumanmilkoligosaccharidesandnglycansinthemilkofchinesemothersregulatethegutmicrobiomeoftheirbreastfedinfantsduringdifferentlactationstages AT jingyuyan fucosylatedhumanmilkoligosaccharidesandnglycansinthemilkofchinesemothersregulatethegutmicrobiomeoftheirbreastfedinfantsduringdifferentlactationstages AT mingli fucosylatedhumanmilkoligosaccharidesandnglycansinthemilkofchinesemothersregulatethegutmicrobiomeoftheirbreastfedinfantsduringdifferentlactationstages |
| _version_ |
1718376031615713280 |