Reliability of serum metabolite concentrations over a 4-month period using a targeted metabolomic approach.

Metabolomics is a promising tool for discovery of novel biomarkers of chronic disease risk in prospective epidemiologic studies. We investigated the between- and within-person variation of the concentrations of 163 serum metabolites over a period of 4 months to evaluate the metabolite reliability ex...

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Autores principales:	Anna Floegel, Dagmar Drogan, Rui Wang-Sattler, Cornelia Prehn, Thomas Illig, Jerzy Adamski, Hans-Georg Joost, Heiner Boeing, Tobias Pischon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/719537e82a3b4ec2bec74a2135026d9a
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spelling	oai:doaj.org-article:719537e82a3b4ec2bec74a2135026d9a2021-11-18T06:52:04ZReliability of serum metabolite concentrations over a 4-month period using a targeted metabolomic approach.1932-620310.1371/journal.pone.0021103https://doaj.org/article/719537e82a3b4ec2bec74a2135026d9a2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21698256/?tool=EBIhttps://doaj.org/toc/1932-6203Metabolomics is a promising tool for discovery of novel biomarkers of chronic disease risk in prospective epidemiologic studies. We investigated the between- and within-person variation of the concentrations of 163 serum metabolites over a period of 4 months to evaluate the metabolite reliability expressed by the intraclass-correlation coefficient (ICC: the ratio of between-person variance and total variance). The analyses were performed with the BIOCRATES AbsoluteIDQ™ targeted metabolomics technology, including acylcarnitines, amino acids, glycerophospholipids, sphingolipids and hexose in 100 healthy individuals from the European Prospective Investigation into Cancer and Nutrition (EPIC)-Potsdam study who had provided two fasting blood samples 4 months apart. Overall, serum reliability of metabolites over a 4-month period was good. The median ICC of the 163 metabolites was 0.57. The highest ICC was observed for hydroxysphingomyelin C14:1 (ICC = 0.85) and the lowest was found for acylcarnitine C3:1 (ICC = 0). Reliability was high for hexose (ICC = 0.76), sphingolipids (median ICC = 0.66; range: 0.24-0.85), amino acids (median ICC = 0.58; range: 0.41-0.72) and glycerophospholipids (median ICC = 0.58; range: 0.03-0.81). Among acylcarnitines, reliability of short and medium chain saturated compounds was good to excellent (ICC range: 0.50-0.81). Serum reliability was lower for most hydroxyacylcarnitines and monounsaturated acylcarnitines (ICC range: 0.11-0.45 and 0.00-0.63, respectively). For most of the metabolites a single measurement may be sufficient for risk assessment in epidemiologic studies with healthy subjects.Anna FloegelDagmar DroganRui Wang-SattlerCornelia PrehnThomas IlligJerzy AdamskiHans-Georg JoostHeiner BoeingTobias PischonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 6, p e21103 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Floegel Dagmar Drogan Rui Wang-Sattler Cornelia Prehn Thomas Illig Jerzy Adamski Hans-Georg Joost Heiner Boeing Tobias Pischon Reliability of serum metabolite concentrations over a 4-month period using a targeted metabolomic approach.
description	Metabolomics is a promising tool for discovery of novel biomarkers of chronic disease risk in prospective epidemiologic studies. We investigated the between- and within-person variation of the concentrations of 163 serum metabolites over a period of 4 months to evaluate the metabolite reliability expressed by the intraclass-correlation coefficient (ICC: the ratio of between-person variance and total variance). The analyses were performed with the BIOCRATES AbsoluteIDQ™ targeted metabolomics technology, including acylcarnitines, amino acids, glycerophospholipids, sphingolipids and hexose in 100 healthy individuals from the European Prospective Investigation into Cancer and Nutrition (EPIC)-Potsdam study who had provided two fasting blood samples 4 months apart. Overall, serum reliability of metabolites over a 4-month period was good. The median ICC of the 163 metabolites was 0.57. The highest ICC was observed for hydroxysphingomyelin C14:1 (ICC = 0.85) and the lowest was found for acylcarnitine C3:1 (ICC = 0). Reliability was high for hexose (ICC = 0.76), sphingolipids (median ICC = 0.66; range: 0.24-0.85), amino acids (median ICC = 0.58; range: 0.41-0.72) and glycerophospholipids (median ICC = 0.58; range: 0.03-0.81). Among acylcarnitines, reliability of short and medium chain saturated compounds was good to excellent (ICC range: 0.50-0.81). Serum reliability was lower for most hydroxyacylcarnitines and monounsaturated acylcarnitines (ICC range: 0.11-0.45 and 0.00-0.63, respectively). For most of the metabolites a single measurement may be sufficient for risk assessment in epidemiologic studies with healthy subjects.
format	article
author	Anna Floegel Dagmar Drogan Rui Wang-Sattler Cornelia Prehn Thomas Illig Jerzy Adamski Hans-Georg Joost Heiner Boeing Tobias Pischon
author_facet	Anna Floegel Dagmar Drogan Rui Wang-Sattler Cornelia Prehn Thomas Illig Jerzy Adamski Hans-Georg Joost Heiner Boeing Tobias Pischon
author_sort	Anna Floegel
title	Reliability of serum metabolite concentrations over a 4-month period using a targeted metabolomic approach.
title_short	Reliability of serum metabolite concentrations over a 4-month period using a targeted metabolomic approach.
title_full	Reliability of serum metabolite concentrations over a 4-month period using a targeted metabolomic approach.
title_fullStr	Reliability of serum metabolite concentrations over a 4-month period using a targeted metabolomic approach.
title_full_unstemmed	Reliability of serum metabolite concentrations over a 4-month period using a targeted metabolomic approach.
title_sort	reliability of serum metabolite concentrations over a 4-month period using a targeted metabolomic approach.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/719537e82a3b4ec2bec74a2135026d9a
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Reliability of serum metabolite concentrations over a 4-month period using a targeted metabolomic approach.

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