Increasing cell density globally enhances the biogenesis of Piwi-interacting RNAs in Bombyx mori germ cells

Abstract Piwi proteins and their bound Piwi-interacting RNAs (piRNAs) are predominantly expressed in the germline and play crucial roles in germline development by silencing transposons and other targets. Bombyx mori BmN4 cells are culturable germ cells that equip the piRNA pathway. Because of the s...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shozo Honda, Phillipe Loher, Keisuke Morichika, Megumi Shigematsu, Takuya Kawamura, Yoriko Kirino, Isidore Rigoutsos, Yohei Kirino
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7196e9b3a1c64e6d884a003b92e7c0b6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7196e9b3a1c64e6d884a003b92e7c0b6
record_format	dspace
spelling	oai:doaj.org-article:7196e9b3a1c64e6d884a003b92e7c0b62021-12-02T12:32:17ZIncreasing cell density globally enhances the biogenesis of Piwi-interacting RNAs in Bombyx mori germ cells10.1038/s41598-017-04429-72045-2322https://doaj.org/article/7196e9b3a1c64e6d884a003b92e7c0b62017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04429-7https://doaj.org/toc/2045-2322Abstract Piwi proteins and their bound Piwi-interacting RNAs (piRNAs) are predominantly expressed in the germline and play crucial roles in germline development by silencing transposons and other targets. Bombyx mori BmN4 cells are culturable germ cells that equip the piRNA pathway. Because of the scarcity of piRNA-expressing culturable cells, BmN4 cells are being utilized for the analyses of piRNA biogenesis. We here report that the piRNA biogenesis in BmN4 cells is regulated by cell density. As cell density increased, the abundance of Piwi proteins and piRNA biogenesis factors was commonly upregulated, resulting in an increased number of perinuclear nuage-like granules where Piwi proteins localize. Along with these phenomena, the abundance of mature piRNAs also globally increased, whereas levels of long piRNA precursor and transposons decreased, suggesting that increasing cell density promotes piRNA biogenesis pathway and that the resultant accumulation of mature piRNAs is functionally significant for transposon silencing. Our study reveals a previously uncharacterized link between cell density and piRNA biogenesis, designates cell density as a critical variable in piRNA studies using BmN4 cell system, and suggests the alteration of cell density as a useful tool to monitor piRNA biogenesis and function.Shozo HondaPhillipe LoherKeisuke MorichikaMegumi ShigematsuTakuya KawamuraYoriko KirinoIsidore RigoutsosYohei KirinoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shozo Honda Phillipe Loher Keisuke Morichika Megumi Shigematsu Takuya Kawamura Yoriko Kirino Isidore Rigoutsos Yohei Kirino Increasing cell density globally enhances the biogenesis of Piwi-interacting RNAs in Bombyx mori germ cells
description	Abstract Piwi proteins and their bound Piwi-interacting RNAs (piRNAs) are predominantly expressed in the germline and play crucial roles in germline development by silencing transposons and other targets. Bombyx mori BmN4 cells are culturable germ cells that equip the piRNA pathway. Because of the scarcity of piRNA-expressing culturable cells, BmN4 cells are being utilized for the analyses of piRNA biogenesis. We here report that the piRNA biogenesis in BmN4 cells is regulated by cell density. As cell density increased, the abundance of Piwi proteins and piRNA biogenesis factors was commonly upregulated, resulting in an increased number of perinuclear nuage-like granules where Piwi proteins localize. Along with these phenomena, the abundance of mature piRNAs also globally increased, whereas levels of long piRNA precursor and transposons decreased, suggesting that increasing cell density promotes piRNA biogenesis pathway and that the resultant accumulation of mature piRNAs is functionally significant for transposon silencing. Our study reveals a previously uncharacterized link between cell density and piRNA biogenesis, designates cell density as a critical variable in piRNA studies using BmN4 cell system, and suggests the alteration of cell density as a useful tool to monitor piRNA biogenesis and function.
format	article
author	Shozo Honda Phillipe Loher Keisuke Morichika Megumi Shigematsu Takuya Kawamura Yoriko Kirino Isidore Rigoutsos Yohei Kirino
author_facet	Shozo Honda Phillipe Loher Keisuke Morichika Megumi Shigematsu Takuya Kawamura Yoriko Kirino Isidore Rigoutsos Yohei Kirino
author_sort	Shozo Honda
title	Increasing cell density globally enhances the biogenesis of Piwi-interacting RNAs in Bombyx mori germ cells
title_short	Increasing cell density globally enhances the biogenesis of Piwi-interacting RNAs in Bombyx mori germ cells
title_full	Increasing cell density globally enhances the biogenesis of Piwi-interacting RNAs in Bombyx mori germ cells
title_fullStr	Increasing cell density globally enhances the biogenesis of Piwi-interacting RNAs in Bombyx mori germ cells
title_full_unstemmed	Increasing cell density globally enhances the biogenesis of Piwi-interacting RNAs in Bombyx mori germ cells
title_sort	increasing cell density globally enhances the biogenesis of piwi-interacting rnas in bombyx mori germ cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7196e9b3a1c64e6d884a003b92e7c0b6
work_keys_str_mv	AT shozohonda increasingcelldensitygloballyenhancesthebiogenesisofpiwiinteractingrnasinbombyxmorigermcells AT phillipeloher increasingcelldensitygloballyenhancesthebiogenesisofpiwiinteractingrnasinbombyxmorigermcells AT keisukemorichika increasingcelldensitygloballyenhancesthebiogenesisofpiwiinteractingrnasinbombyxmorigermcells AT megumishigematsu increasingcelldensitygloballyenhancesthebiogenesisofpiwiinteractingrnasinbombyxmorigermcells AT takuyakawamura increasingcelldensitygloballyenhancesthebiogenesisofpiwiinteractingrnasinbombyxmorigermcells AT yorikokirino increasingcelldensitygloballyenhancesthebiogenesisofpiwiinteractingrnasinbombyxmorigermcells AT isidorerigoutsos increasingcelldensitygloballyenhancesthebiogenesisofpiwiinteractingrnasinbombyxmorigermcells AT yoheikirino increasingcelldensitygloballyenhancesthebiogenesisofpiwiinteractingrnasinbombyxmorigermcells
_version_	1718394100837777408

Increasing cell density globally enhances the biogenesis of Piwi-interacting RNAs in Bombyx mori germ cells

Ejemplares similares