Evaluating RNA Structural Flexibility: Viruses Lead the Way

Our understanding of RNA structure has lagged behind that of proteins and most other biological polymers, largely because of its ability to adopt multiple, and often very different, functional conformations within a single molecule. Flexibility and multifunctionality appear to be its hallmarks. Conv...

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Autores principales:	Connor W. Fairman, Andrew M. L. Lever, Julia C. Kenyon
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	RNA structure RNA flexibility RNA viruses SHAPE proximity ligation NMR Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/719a716a176d44d1b2984ae369a4b33b
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spelling	oai:doaj.org-article:719a716a176d44d1b2984ae369a4b33b2021-11-25T19:12:32ZEvaluating RNA Structural Flexibility: Viruses Lead the Way10.3390/v131121301999-4915https://doaj.org/article/719a716a176d44d1b2984ae369a4b33b2021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2130https://doaj.org/toc/1999-4915Our understanding of RNA structure has lagged behind that of proteins and most other biological polymers, largely because of its ability to adopt multiple, and often very different, functional conformations within a single molecule. Flexibility and multifunctionality appear to be its hallmarks. Conventional biochemical and biophysical techniques all have limitations in solving RNA structure and to address this in recent years we have seen the emergence of a wide diversity of techniques applied to RNA structural analysis and an accompanying appreciation of its ubiquity and versatility. Viral RNA is a particularly productive area to study in that this economy of function within a single molecule admirably suits the minimalist lifestyle of viruses. Here, we review the major techniques that are being used to elucidate RNA conformational flexibility and exemplify how the structure and function are, as in all biology, tightly linked.Connor W. FairmanAndrew M. L. LeverJulia C. KenyonMDPI AGarticleRNA structureRNA flexibilityRNA virusesSHAPEproximity ligationNMRMicrobiologyQR1-502ENViruses, Vol 13, Iss 2130, p 2130 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	RNA structure RNA flexibility RNA viruses SHAPE proximity ligation NMR Microbiology QR1-502
spellingShingle	RNA structure RNA flexibility RNA viruses SHAPE proximity ligation NMR Microbiology QR1-502 Connor W. Fairman Andrew M. L. Lever Julia C. Kenyon Evaluating RNA Structural Flexibility: Viruses Lead the Way
description	Our understanding of RNA structure has lagged behind that of proteins and most other biological polymers, largely because of its ability to adopt multiple, and often very different, functional conformations within a single molecule. Flexibility and multifunctionality appear to be its hallmarks. Conventional biochemical and biophysical techniques all have limitations in solving RNA structure and to address this in recent years we have seen the emergence of a wide diversity of techniques applied to RNA structural analysis and an accompanying appreciation of its ubiquity and versatility. Viral RNA is a particularly productive area to study in that this economy of function within a single molecule admirably suits the minimalist lifestyle of viruses. Here, we review the major techniques that are being used to elucidate RNA conformational flexibility and exemplify how the structure and function are, as in all biology, tightly linked.
format	article
author	Connor W. Fairman Andrew M. L. Lever Julia C. Kenyon
author_facet	Connor W. Fairman Andrew M. L. Lever Julia C. Kenyon
author_sort	Connor W. Fairman
title	Evaluating RNA Structural Flexibility: Viruses Lead the Way
title_short	Evaluating RNA Structural Flexibility: Viruses Lead the Way
title_full	Evaluating RNA Structural Flexibility: Viruses Lead the Way
title_fullStr	Evaluating RNA Structural Flexibility: Viruses Lead the Way
title_full_unstemmed	Evaluating RNA Structural Flexibility: Viruses Lead the Way
title_sort	evaluating rna structural flexibility: viruses lead the way
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/719a716a176d44d1b2984ae369a4b33b
work_keys_str_mv	AT connorwfairman evaluatingrnastructuralflexibilityvirusesleadtheway AT andrewmllever evaluatingrnastructuralflexibilityvirusesleadtheway AT juliackenyon evaluatingrnastructuralflexibilityvirusesleadtheway
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Evaluating RNA Structural Flexibility: Viruses Lead the Way

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