Bacterial membrane vesicles from Acinetobacter baumannii induced by ceftazidime are more virulent than those induced by imipenem

Patients with Acinetobacter baumannii bacteremia treated with antipseudomonal cephalosporins showed higher 14-day mortality than patients treated with antipseudomonal carbapenems. We hypothesized that the bacterial membrane vesicles (BMVs) induced by antipseudomonal cephalosporins are more virulent...

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Autores principales:	Chun-Hsiang Chiu, Yi-Tzu Lee, Yu-Chun Lin, Shu-Chen Kuo, Ya-Sung Yang, Yung-Chih Wang, Yu-Han Liu, Jung-Chung Lin, Feng-Yee Chang, Te-Li Chen
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	bacterial membrane vesicles acinetobacter baumannii ceftazidime imipenem virulence Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/719c766ad5054befbe957d4b7ad9fac0
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spelling	oai:doaj.org-article:719c766ad5054befbe957d4b7ad9fac02021-11-17T14:21:57ZBacterial membrane vesicles from Acinetobacter baumannii induced by ceftazidime are more virulent than those induced by imipenem2150-55942150-560810.1080/21505594.2020.1726593https://doaj.org/article/719c766ad5054befbe957d4b7ad9fac02020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1726593https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Patients with Acinetobacter baumannii bacteremia treated with antipseudomonal cephalosporins showed higher 14-day mortality than patients treated with antipseudomonal carbapenems. We hypothesized that the bacterial membrane vesicles (BMVs) induced by antipseudomonal cephalosporins are more virulent than BMVs induced by antipseudomonal carbapenems. To simulate the clinical condition with inadequate antimicrobial treatment, carbapenem-resistant A. baumannii was treated with ceftazidime (an antipseudomonal cephalosporin) or imipenem (an antipseudomonal carbapenem) at 1/2 the minimum inhibitory concentration. BMVs and BMV-carried lipopolysaccharide were measured by nanoparticle tracking analysis and western blotting, respectively. Cytokine expression in RAW264.7 macrophages or mice serum induced by the BMVs was determined by ELISA, fluorescent bead-based immunoassay or western blotting. The virulence of the BMVs was assessed in mice. Liquid chromatography tandem-mass spectrometry was used to determine the protein contents of the BMVs. We found that ceftazidime induced a higher number of BMVs (CAZ-BMV), which carried more LPS, and induced higher expression levels of iNOS, IL-1β, and IL-6 in macrophages, higher expression of many cytokines in mice, more neutrophil infiltration in lung interstitium, and higher mortality in mice than imipenem-induced BMVs (IMP-BMV). When adjusted to same amount of LPS, CAZ-BMV still led to higher mortality than IMP-BMV. Proteomic analysis revealed different protein contents in CAZ-BMV and IMP-BMV. In conclusion, A. baumannii BMVs induced by ceftazidime are more virulent than BMVs induced by imipenem.Chun-Hsiang ChiuYi-Tzu LeeYu-Chun LinShu-Chen KuoYa-Sung YangYung-Chih WangYu-Han LiuJung-Chung LinFeng-Yee ChangTe-Li ChenTaylor & Francis Grouparticlebacterial membrane vesiclesacinetobacter baumanniiceftazidimeimipenemvirulenceInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 145-158 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial membrane vesicles acinetobacter baumannii ceftazidime imipenem virulence Infectious and parasitic diseases RC109-216
spellingShingle	bacterial membrane vesicles acinetobacter baumannii ceftazidime imipenem virulence Infectious and parasitic diseases RC109-216 Chun-Hsiang Chiu Yi-Tzu Lee Yu-Chun Lin Shu-Chen Kuo Ya-Sung Yang Yung-Chih Wang Yu-Han Liu Jung-Chung Lin Feng-Yee Chang Te-Li Chen Bacterial membrane vesicles from Acinetobacter baumannii induced by ceftazidime are more virulent than those induced by imipenem
description	Patients with Acinetobacter baumannii bacteremia treated with antipseudomonal cephalosporins showed higher 14-day mortality than patients treated with antipseudomonal carbapenems. We hypothesized that the bacterial membrane vesicles (BMVs) induced by antipseudomonal cephalosporins are more virulent than BMVs induced by antipseudomonal carbapenems. To simulate the clinical condition with inadequate antimicrobial treatment, carbapenem-resistant A. baumannii was treated with ceftazidime (an antipseudomonal cephalosporin) or imipenem (an antipseudomonal carbapenem) at 1/2 the minimum inhibitory concentration. BMVs and BMV-carried lipopolysaccharide were measured by nanoparticle tracking analysis and western blotting, respectively. Cytokine expression in RAW264.7 macrophages or mice serum induced by the BMVs was determined by ELISA, fluorescent bead-based immunoassay or western blotting. The virulence of the BMVs was assessed in mice. Liquid chromatography tandem-mass spectrometry was used to determine the protein contents of the BMVs. We found that ceftazidime induced a higher number of BMVs (CAZ-BMV), which carried more LPS, and induced higher expression levels of iNOS, IL-1β, and IL-6 in macrophages, higher expression of many cytokines in mice, more neutrophil infiltration in lung interstitium, and higher mortality in mice than imipenem-induced BMVs (IMP-BMV). When adjusted to same amount of LPS, CAZ-BMV still led to higher mortality than IMP-BMV. Proteomic analysis revealed different protein contents in CAZ-BMV and IMP-BMV. In conclusion, A. baumannii BMVs induced by ceftazidime are more virulent than BMVs induced by imipenem.
format	article
author	Chun-Hsiang Chiu Yi-Tzu Lee Yu-Chun Lin Shu-Chen Kuo Ya-Sung Yang Yung-Chih Wang Yu-Han Liu Jung-Chung Lin Feng-Yee Chang Te-Li Chen
author_facet	Chun-Hsiang Chiu Yi-Tzu Lee Yu-Chun Lin Shu-Chen Kuo Ya-Sung Yang Yung-Chih Wang Yu-Han Liu Jung-Chung Lin Feng-Yee Chang Te-Li Chen
author_sort	Chun-Hsiang Chiu
title	Bacterial membrane vesicles from Acinetobacter baumannii induced by ceftazidime are more virulent than those induced by imipenem
title_short	Bacterial membrane vesicles from Acinetobacter baumannii induced by ceftazidime are more virulent than those induced by imipenem
title_full	Bacterial membrane vesicles from Acinetobacter baumannii induced by ceftazidime are more virulent than those induced by imipenem
title_fullStr	Bacterial membrane vesicles from Acinetobacter baumannii induced by ceftazidime are more virulent than those induced by imipenem
title_full_unstemmed	Bacterial membrane vesicles from Acinetobacter baumannii induced by ceftazidime are more virulent than those induced by imipenem
title_sort	bacterial membrane vesicles from acinetobacter baumannii induced by ceftazidime are more virulent than those induced by imipenem
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/719c766ad5054befbe957d4b7ad9fac0
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Bacterial membrane vesicles from Acinetobacter baumannii induced by ceftazidime are more virulent than those induced by imipenem

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