New Insights into Dissemination and Variation of the Health Care-Associated Pathogen <named-content content-type="genus-species">Acinetobacter baumannii</named-content> from Genomic Analysis

ABSTRACT Acinetobacter baumannii is a globally important nosocomial pathogen characterized by an increasing incidence of multidrug resistance. Routes of dissemination and gene flow among health care facilities are poorly resolved and are important for understanding the epidemiology of A. baumannii,...

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Autores principales:	Meredith S. Wright, Daniel H. Haft, Derek M. Harkins, Federico Perez, Kristine M. Hujer, Saralee Bajaksouzian, Michael F. Benard, Michael R. Jacobs, Robert A. Bonomo, Mark D. Adams
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/71a65212208f4a8588f88dc40134a600
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spelling	oai:doaj.org-article:71a65212208f4a8588f88dc40134a6002021-11-15T15:45:10ZNew Insights into Dissemination and Variation of the Health Care-Associated Pathogen <named-content content-type="genus-species">Acinetobacter baumannii</named-content> from Genomic Analysis10.1128/mBio.00963-132150-7511https://doaj.org/article/71a65212208f4a8588f88dc40134a6002014-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00963-13https://doaj.org/toc/2150-7511ABSTRACT Acinetobacter baumannii is a globally important nosocomial pathogen characterized by an increasing incidence of multidrug resistance. Routes of dissemination and gene flow among health care facilities are poorly resolved and are important for understanding the epidemiology of A. baumannii, minimizing disease transmission, and improving patient outcomes. We used whole-genome sequencing to assess diversity and genome dynamics in 49 isolates from one United States hospital system during one year from 2007 to 2008. Core single-nucleotide-variant-based phylogenetic analysis revealed multiple founder strains and multiple independent strains recovered from the same patient yet was insufficient to fully resolve strain relationships, where gene content and insertion sequence patterns added additional discriminatory power. Gene content comparisons illustrated extensive and redundant antibiotic resistance gene carriage and direct evidence of gene transfer, recombination, gene loss, and mutation. Evidence of barriers to gene flow among hospital components was not found, suggesting complex mixing of strains and a large reservoir of A. baumannii strains capable of colonizing patients. IMPORTANCE Genome sequencing was used to characterize multidrug-resistant Acinetobacter baumannii strains from one United States hospital system during a 1-year period to better understand how A. baumannii strains that cause infection are related to one another. Extensive variation in gene content was found, even among strains that were very closely related phylogenetically and epidemiologically. Several mechanisms contributed to this diversity, including transfer of mobile genetic elements, mobilization of insertion sequences, insertion sequence-mediated deletions, and genome-wide homologous recombination. Variation in gene content, however, lacked clear spatial or temporal patterns, suggesting a diverse pool of circulating strains with considerable interaction between strains and hospital locations. Widespread genetic variation among strains from the same hospital and even the same patient, particularly involving antibiotic resistance genes, reinforces the need for molecular diagnostic testing and genomic analysis to determine resistance profiles, rather than a reliance primarily on strain typing and antimicrobial resistance phenotypes for epidemiological studies.Meredith S. WrightDaniel H. HaftDerek M. HarkinsFederico PerezKristine M. HujerSaralee BajaksouzianMichael F. BenardMichael R. JacobsRobert A. BonomoMark D. AdamsAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 1 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Meredith S. Wright Daniel H. Haft Derek M. Harkins Federico Perez Kristine M. Hujer Saralee Bajaksouzian Michael F. Benard Michael R. Jacobs Robert A. Bonomo Mark D. Adams New Insights into Dissemination and Variation of the Health Care-Associated Pathogen <named-content content-type="genus-species">Acinetobacter baumannii</named-content> from Genomic Analysis
description	ABSTRACT Acinetobacter baumannii is a globally important nosocomial pathogen characterized by an increasing incidence of multidrug resistance. Routes of dissemination and gene flow among health care facilities are poorly resolved and are important for understanding the epidemiology of A. baumannii, minimizing disease transmission, and improving patient outcomes. We used whole-genome sequencing to assess diversity and genome dynamics in 49 isolates from one United States hospital system during one year from 2007 to 2008. Core single-nucleotide-variant-based phylogenetic analysis revealed multiple founder strains and multiple independent strains recovered from the same patient yet was insufficient to fully resolve strain relationships, where gene content and insertion sequence patterns added additional discriminatory power. Gene content comparisons illustrated extensive and redundant antibiotic resistance gene carriage and direct evidence of gene transfer, recombination, gene loss, and mutation. Evidence of barriers to gene flow among hospital components was not found, suggesting complex mixing of strains and a large reservoir of A. baumannii strains capable of colonizing patients. IMPORTANCE Genome sequencing was used to characterize multidrug-resistant Acinetobacter baumannii strains from one United States hospital system during a 1-year period to better understand how A. baumannii strains that cause infection are related to one another. Extensive variation in gene content was found, even among strains that were very closely related phylogenetically and epidemiologically. Several mechanisms contributed to this diversity, including transfer of mobile genetic elements, mobilization of insertion sequences, insertion sequence-mediated deletions, and genome-wide homologous recombination. Variation in gene content, however, lacked clear spatial or temporal patterns, suggesting a diverse pool of circulating strains with considerable interaction between strains and hospital locations. Widespread genetic variation among strains from the same hospital and even the same patient, particularly involving antibiotic resistance genes, reinforces the need for molecular diagnostic testing and genomic analysis to determine resistance profiles, rather than a reliance primarily on strain typing and antimicrobial resistance phenotypes for epidemiological studies.
format	article
author	Meredith S. Wright Daniel H. Haft Derek M. Harkins Federico Perez Kristine M. Hujer Saralee Bajaksouzian Michael F. Benard Michael R. Jacobs Robert A. Bonomo Mark D. Adams
author_facet	Meredith S. Wright Daniel H. Haft Derek M. Harkins Federico Perez Kristine M. Hujer Saralee Bajaksouzian Michael F. Benard Michael R. Jacobs Robert A. Bonomo Mark D. Adams
author_sort	Meredith S. Wright
title	New Insights into Dissemination and Variation of the Health Care-Associated Pathogen <named-content content-type="genus-species">Acinetobacter baumannii</named-content> from Genomic Analysis
title_short	New Insights into Dissemination and Variation of the Health Care-Associated Pathogen <named-content content-type="genus-species">Acinetobacter baumannii</named-content> from Genomic Analysis
title_full	New Insights into Dissemination and Variation of the Health Care-Associated Pathogen <named-content content-type="genus-species">Acinetobacter baumannii</named-content> from Genomic Analysis
title_fullStr	New Insights into Dissemination and Variation of the Health Care-Associated Pathogen <named-content content-type="genus-species">Acinetobacter baumannii</named-content> from Genomic Analysis
title_full_unstemmed	New Insights into Dissemination and Variation of the Health Care-Associated Pathogen <named-content content-type="genus-species">Acinetobacter baumannii</named-content> from Genomic Analysis
title_sort	new insights into dissemination and variation of the health care-associated pathogen <named-content content-type="genus-species">acinetobacter baumannii</named-content> from genomic analysis
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/71a65212208f4a8588f88dc40134a600
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New Insights into Dissemination and Variation of the Health Care-Associated Pathogen <named-content content-type="genus-species">Acinetobacter baumannii</named-content> from Genomic Analysis

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