Efficient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variant

Abstract Thermophilic organisms are extensively studied in industrial biotechnology, for exploration of the limits of life, and in other contexts. Their optimal growth at high temperatures presents a challenge for the development of genetic tools for their genome editing, since genetic markers and s...

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Autores principales: Bjorn Thor Adalsteinsson, Thordis Kristjansdottir, William Merre, Alexandra Helleux, Julia Dusaucy, Mathilde Tourigny, Olafur Fridjonsson, Gudmundur Oli Hreggvidsson
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/71aa805bce4a4201afdca651fc406aba
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