Coagulation factor IX analysis in bioreactor cell culture supernatant predicts quality of the purified product

Zacchi et al. attempt to bridge the gap between understanding the impact of process variables and determination of critical post-translational modifications on a highly complex glycoprotein, factor IX. They implement an LC-MS/MS approach based on data independent acquisition which allows measurement...

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Autores principales:	Lucia F. Zacchi, Dinora Roche-Recinos, Cassandra L. Pegg, Toan K. Phung, Mark Napoli, Campbell Aitken, Vanessa Sandford, Stephen M. Mahler, Yih Yean Lee, Benjamin L. Schulz, Christopher B. Howard
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/71aba8fb8f8b4392a1a05cbdb5d2e86f
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spelling	oai:doaj.org-article:71aba8fb8f8b4392a1a05cbdb5d2e86f2021-12-02T13:24:24ZCoagulation factor IX analysis in bioreactor cell culture supernatant predicts quality of the purified product10.1038/s42003-021-01903-x2399-3642https://doaj.org/article/71aba8fb8f8b4392a1a05cbdb5d2e86f2021-03-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01903-xhttps://doaj.org/toc/2399-3642Zacchi et al. attempt to bridge the gap between understanding the impact of process variables and determination of critical post-translational modifications on a highly complex glycoprotein, factor IX. They implement an LC-MS/MS approach based on data independent acquisition which allows measurement of all detectable peptides, even with heterogeneous modifications.Lucia F. ZacchiDinora Roche-RecinosCassandra L. PeggToan K. PhungMark NapoliCampbell AitkenVanessa SandfordStephen M. MahlerYih Yean LeeBenjamin L. SchulzChristopher B. HowardNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Lucia F. Zacchi Dinora Roche-Recinos Cassandra L. Pegg Toan K. Phung Mark Napoli Campbell Aitken Vanessa Sandford Stephen M. Mahler Yih Yean Lee Benjamin L. Schulz Christopher B. Howard Coagulation factor IX analysis in bioreactor cell culture supernatant predicts quality of the purified product
description	Zacchi et al. attempt to bridge the gap between understanding the impact of process variables and determination of critical post-translational modifications on a highly complex glycoprotein, factor IX. They implement an LC-MS/MS approach based on data independent acquisition which allows measurement of all detectable peptides, even with heterogeneous modifications.
format	article
author	Lucia F. Zacchi Dinora Roche-Recinos Cassandra L. Pegg Toan K. Phung Mark Napoli Campbell Aitken Vanessa Sandford Stephen M. Mahler Yih Yean Lee Benjamin L. Schulz Christopher B. Howard
author_facet	Lucia F. Zacchi Dinora Roche-Recinos Cassandra L. Pegg Toan K. Phung Mark Napoli Campbell Aitken Vanessa Sandford Stephen M. Mahler Yih Yean Lee Benjamin L. Schulz Christopher B. Howard
author_sort	Lucia F. Zacchi
title	Coagulation factor IX analysis in bioreactor cell culture supernatant predicts quality of the purified product
title_short	Coagulation factor IX analysis in bioreactor cell culture supernatant predicts quality of the purified product
title_full	Coagulation factor IX analysis in bioreactor cell culture supernatant predicts quality of the purified product
title_fullStr	Coagulation factor IX analysis in bioreactor cell culture supernatant predicts quality of the purified product
title_full_unstemmed	Coagulation factor IX analysis in bioreactor cell culture supernatant predicts quality of the purified product
title_sort	coagulation factor ix analysis in bioreactor cell culture supernatant predicts quality of the purified product
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/71aba8fb8f8b4392a1a05cbdb5d2e86f
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Coagulation factor IX analysis in bioreactor cell culture supernatant predicts quality of the purified product

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