RNA structure-wide discovery of functional interactions with multiplexed RNA motif library

Structured RNA motifs can be obtained by structure probing, duplex capture, and motif prediction. Here the authors develop a multiplexed affinity assay system to identify functional protein interactors from an RNA structure library with validated or predicted RNA motifs.

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Detalles Bibliográficos
Autores principales: Kaoru R. Komatsu, Toshiki Taya, Sora Matsumoto, Emi Miyashita, Shunnichi Kashida, Hirohide Saito
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/71ac6f2904824fc8bf435fd6f84ffb58
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Sumario:Structured RNA motifs can be obtained by structure probing, duplex capture, and motif prediction. Here the authors develop a multiplexed affinity assay system to identify functional protein interactors from an RNA structure library with validated or predicted RNA motifs.