Autoinducers act as biological timers in Vibrio harveyi.

Quorum sensing regulates cell density-dependent phenotypes and involves the synthesis, excretion and detection of so-called autoinducers. Vibrio harveyi strain ATCC BAA-1116 (recently reclassified as Vibrio campbellii), one of the best-characterized model organisms for the study of quorum sensing, p...

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Autores principales:	Claudia Anetzberger, Matthias Reiger, Agnes Fekete, Ursula Schell, Nina Stambrau, Laure Plener, Joachim Kopka, Phillippe Schmitt-Kopplin, Hubert Hilbi, Kirsten Jung
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/71b617aaf9dd47e0b22a32f57a090469
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spelling	oai:doaj.org-article:71b617aaf9dd47e0b22a32f57a0904692021-11-18T08:10:50ZAutoinducers act as biological timers in Vibrio harveyi.1932-620310.1371/journal.pone.0048310https://doaj.org/article/71b617aaf9dd47e0b22a32f57a0904692012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23110227/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Quorum sensing regulates cell density-dependent phenotypes and involves the synthesis, excretion and detection of so-called autoinducers. Vibrio harveyi strain ATCC BAA-1116 (recently reclassified as Vibrio campbellii), one of the best-characterized model organisms for the study of quorum sensing, produces and responds to three autoinducers. HAI-1, AI-2 and CAI-1 are recognized by different receptors, but all information is channeled into the same signaling cascade, which controls a specific set of genes. Here we examine temporal variations of availability and concentration of the three autoinducers in V. harveyi, and monitor the phenotypes they regulate, from the early exponential to the stationary growth phase in liquid culture. Specifically, the exponential growth phase is characterized by an increase in AI-2 and the induction of bioluminescence, while HAI-1 and CAI-1 are undetectable prior to the late exponential growth phase. CAI-1 activity reaches its maximum upon entry into stationary phase, while molar concentrations of AI-2 and HAI-1 become approximately equal. Similarly, autoinducer-dependent exoproteolytic activity increases at the transition into stationary phase. These findings are reflected in temporal alterations in expression of the luxR gene that encodes the master regulator LuxR, and of four autoinducer-regulated genes during growth. Moreover, in vitro phosphorylation assays reveal a tight correlation between the HAI-1/AI-2 ratio as input and levels of receptor-mediated phosphorylation of LuxU as output. Our study supports a model in which the combinations of autoinducers available, rather than cell density per se, determine the timing of various processes in V. harveyi populations.Claudia AnetzbergerMatthias ReigerAgnes FeketeUrsula SchellNina StambrauLaure PlenerJoachim KopkaPhillippe Schmitt-KopplinHubert HilbiKirsten JungPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e48310 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claudia Anetzberger Matthias Reiger Agnes Fekete Ursula Schell Nina Stambrau Laure Plener Joachim Kopka Phillippe Schmitt-Kopplin Hubert Hilbi Kirsten Jung Autoinducers act as biological timers in Vibrio harveyi.
description	Quorum sensing regulates cell density-dependent phenotypes and involves the synthesis, excretion and detection of so-called autoinducers. Vibrio harveyi strain ATCC BAA-1116 (recently reclassified as Vibrio campbellii), one of the best-characterized model organisms for the study of quorum sensing, produces and responds to three autoinducers. HAI-1, AI-2 and CAI-1 are recognized by different receptors, but all information is channeled into the same signaling cascade, which controls a specific set of genes. Here we examine temporal variations of availability and concentration of the three autoinducers in V. harveyi, and monitor the phenotypes they regulate, from the early exponential to the stationary growth phase in liquid culture. Specifically, the exponential growth phase is characterized by an increase in AI-2 and the induction of bioluminescence, while HAI-1 and CAI-1 are undetectable prior to the late exponential growth phase. CAI-1 activity reaches its maximum upon entry into stationary phase, while molar concentrations of AI-2 and HAI-1 become approximately equal. Similarly, autoinducer-dependent exoproteolytic activity increases at the transition into stationary phase. These findings are reflected in temporal alterations in expression of the luxR gene that encodes the master regulator LuxR, and of four autoinducer-regulated genes during growth. Moreover, in vitro phosphorylation assays reveal a tight correlation between the HAI-1/AI-2 ratio as input and levels of receptor-mediated phosphorylation of LuxU as output. Our study supports a model in which the combinations of autoinducers available, rather than cell density per se, determine the timing of various processes in V. harveyi populations.
format	article
author	Claudia Anetzberger Matthias Reiger Agnes Fekete Ursula Schell Nina Stambrau Laure Plener Joachim Kopka Phillippe Schmitt-Kopplin Hubert Hilbi Kirsten Jung
author_facet	Claudia Anetzberger Matthias Reiger Agnes Fekete Ursula Schell Nina Stambrau Laure Plener Joachim Kopka Phillippe Schmitt-Kopplin Hubert Hilbi Kirsten Jung
author_sort	Claudia Anetzberger
title	Autoinducers act as biological timers in Vibrio harveyi.
title_short	Autoinducers act as biological timers in Vibrio harveyi.
title_full	Autoinducers act as biological timers in Vibrio harveyi.
title_fullStr	Autoinducers act as biological timers in Vibrio harveyi.
title_full_unstemmed	Autoinducers act as biological timers in Vibrio harveyi.
title_sort	autoinducers act as biological timers in vibrio harveyi.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/71b617aaf9dd47e0b22a32f57a090469
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Autoinducers act as biological timers in Vibrio harveyi.

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