Integrated analysis of long non-coding RNA-microRNA-mRNA competing endogenous RNAregulatory networks in thromboangiitis obliterans

Thromboangiitis obliterans (TAO) is a non-atherosclerotic, segmental, chronic vascular inflammatory disease. Our aim was to explore the underlying mechanisms of long non-coding RNA (lncRNA)-related competing endogenous RNAs (ceRNAs) in TAO. Six blood samples were collected from patients with TAO and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bo Chen, Ying Deng, Bo Wang, Zhongyi Tian, Jindong Tong, Bo Yu, Weijun Shi, Jingdong Tang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	thromboangiitis obliterans lncrna mirna cerna network Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/71b851d29c2544cb81704338b72a781d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:71b851d29c2544cb81704338b72a781d
record_format	dspace
spelling	oai:doaj.org-article:71b851d29c2544cb81704338b72a781d2021-11-26T11:19:49ZIntegrated analysis of long non-coding RNA-microRNA-mRNA competing endogenous RNAregulatory networks in thromboangiitis obliterans2165-59792165-598710.1080/21655979.2021.2002497https://doaj.org/article/71b851d29c2544cb81704338b72a781d2021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2002497https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Thromboangiitis obliterans (TAO) is a non-atherosclerotic, segmental, chronic vascular inflammatory disease. Our aim was to explore the underlying mechanisms of long non-coding RNA (lncRNA)-related competing endogenous RNAs (ceRNAs) in TAO. Six blood samples were collected from patients with TAO and healthy individuals (three for each category). Total RNA was extracted from the blood of each participant and sequenced. Differentially expressed lncRNAs (DE-lncRNAs) and miRNAs (DE-miRNAs) were screened, and ceRNA networks associated with TAO were constructed. Thereafter, the genes in the ceRNA network were subjected to functional analyses. Finally, a ceRNA relationship (lncRNA NEAT1-hsa-miR-1-3p-mRNA GNA12) was selected for further validation. Analysis revealed that 347 DE-lncRNAs (150 downregulated and 197 upregulated) and 16 DE-miRNAs (3 downregulated and 13 upregulated) were identified in TAO. Further, TAO-associated ceRNA networks, which included 219 lncRNAs, 6 miRNAs, and 53 mRNAs, were proposed and subjected to gene annotation and pathway analysis. Additionally, NEAT1 and GNA12 levels were significantly upregulated, while miR-1-3p levels were evidently downregulated in TAO patients, as compared with those in healthy controls. Dual luciferase reporter assays showed that NEAT1, miR-1-3p, and GNA12 interacted with each other. We report potential TAO-associated ceRNA regulatory networks and suggest activation of NEAT1/miR-1-3p/GNA12 signaling as a novel mechanism for TAO progression.Bo ChenYing DengBo WangZhongyi TianJindong TongBo YuWeijun ShiJingdong TangTaylor & Francis Grouparticlethromboangiitis obliteranslncrnamirnacerna networkBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	thromboangiitis obliterans lncrna mirna cerna network Biotechnology TP248.13-248.65
spellingShingle	thromboangiitis obliterans lncrna mirna cerna network Biotechnology TP248.13-248.65 Bo Chen Ying Deng Bo Wang Zhongyi Tian Jindong Tong Bo Yu Weijun Shi Jingdong Tang Integrated analysis of long non-coding RNA-microRNA-mRNA competing endogenous RNAregulatory networks in thromboangiitis obliterans
description	Thromboangiitis obliterans (TAO) is a non-atherosclerotic, segmental, chronic vascular inflammatory disease. Our aim was to explore the underlying mechanisms of long non-coding RNA (lncRNA)-related competing endogenous RNAs (ceRNAs) in TAO. Six blood samples were collected from patients with TAO and healthy individuals (three for each category). Total RNA was extracted from the blood of each participant and sequenced. Differentially expressed lncRNAs (DE-lncRNAs) and miRNAs (DE-miRNAs) were screened, and ceRNA networks associated with TAO were constructed. Thereafter, the genes in the ceRNA network were subjected to functional analyses. Finally, a ceRNA relationship (lncRNA NEAT1-hsa-miR-1-3p-mRNA GNA12) was selected for further validation. Analysis revealed that 347 DE-lncRNAs (150 downregulated and 197 upregulated) and 16 DE-miRNAs (3 downregulated and 13 upregulated) were identified in TAO. Further, TAO-associated ceRNA networks, which included 219 lncRNAs, 6 miRNAs, and 53 mRNAs, were proposed and subjected to gene annotation and pathway analysis. Additionally, NEAT1 and GNA12 levels were significantly upregulated, while miR-1-3p levels were evidently downregulated in TAO patients, as compared with those in healthy controls. Dual luciferase reporter assays showed that NEAT1, miR-1-3p, and GNA12 interacted with each other. We report potential TAO-associated ceRNA regulatory networks and suggest activation of NEAT1/miR-1-3p/GNA12 signaling as a novel mechanism for TAO progression.
format	article
author	Bo Chen Ying Deng Bo Wang Zhongyi Tian Jindong Tong Bo Yu Weijun Shi Jingdong Tang
author_facet	Bo Chen Ying Deng Bo Wang Zhongyi Tian Jindong Tong Bo Yu Weijun Shi Jingdong Tang
author_sort	Bo Chen
title	Integrated analysis of long non-coding RNA-microRNA-mRNA competing endogenous RNAregulatory networks in thromboangiitis obliterans
title_short	Integrated analysis of long non-coding RNA-microRNA-mRNA competing endogenous RNAregulatory networks in thromboangiitis obliterans
title_full	Integrated analysis of long non-coding RNA-microRNA-mRNA competing endogenous RNAregulatory networks in thromboangiitis obliterans
title_fullStr	Integrated analysis of long non-coding RNA-microRNA-mRNA competing endogenous RNAregulatory networks in thromboangiitis obliterans
title_full_unstemmed	Integrated analysis of long non-coding RNA-microRNA-mRNA competing endogenous RNAregulatory networks in thromboangiitis obliterans
title_sort	integrated analysis of long non-coding rna-microrna-mrna competing endogenous rnaregulatory networks in thromboangiitis obliterans
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/71b851d29c2544cb81704338b72a781d
work_keys_str_mv	AT bochen integratedanalysisoflongnoncodingrnamicrornamrnacompetingendogenousrnaregulatorynetworksinthromboangiitisobliterans AT yingdeng integratedanalysisoflongnoncodingrnamicrornamrnacompetingendogenousrnaregulatorynetworksinthromboangiitisobliterans AT bowang integratedanalysisoflongnoncodingrnamicrornamrnacompetingendogenousrnaregulatorynetworksinthromboangiitisobliterans AT zhongyitian integratedanalysisoflongnoncodingrnamicrornamrnacompetingendogenousrnaregulatorynetworksinthromboangiitisobliterans AT jindongtong integratedanalysisoflongnoncodingrnamicrornamrnacompetingendogenousrnaregulatorynetworksinthromboangiitisobliterans AT boyu integratedanalysisoflongnoncodingrnamicrornamrnacompetingendogenousrnaregulatorynetworksinthromboangiitisobliterans AT weijunshi integratedanalysisoflongnoncodingrnamicrornamrnacompetingendogenousrnaregulatorynetworksinthromboangiitisobliterans AT jingdongtang integratedanalysisoflongnoncodingrnamicrornamrnacompetingendogenousrnaregulatorynetworksinthromboangiitisobliterans
_version_	1718409479828013056

Integrated analysis of long non-coding RNA-microRNA-mRNA competing endogenous RNAregulatory networks in thromboangiitis obliterans

Ejemplares similares