Protective effects of broccoli extracts and sulforaphane against hydrogen peroxide induced oxidative stress in B16 cells

The antioxidant activity of broccoli extracts and its purified compounds (polyphenols and purified sulforaphane (PSF)) was investigated. Broccoli extracts and SF protected B16 cells from H2O2-induced oxidative stress through increasing total antioxidant capacity (T-AOC) and activities of NADPH dehyd...

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Autores principales:	Yao Zhang, Chengzhi Lv, Juan Sun, Xinjie Song, Nyasha Makaza, Yuanfeng Wu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Broccoli extracts Sulforaphane Polyphenols Oxidative stress Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/71bb06fefa1342a5bd2fe7818fa504e7
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spelling	oai:doaj.org-article:71bb06fefa1342a5bd2fe7818fa504e72021-11-12T04:28:49ZProtective effects of broccoli extracts and sulforaphane against hydrogen peroxide induced oxidative stress in B16 cells1756-464610.1016/j.jff.2021.104833https://doaj.org/article/71bb06fefa1342a5bd2fe7818fa504e72021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1756464621004825https://doaj.org/toc/1756-4646The antioxidant activity of broccoli extracts and its purified compounds (polyphenols and purified sulforaphane (PSF)) was investigated. Broccoli extracts and SF protected B16 cells from H2O2-induced oxidative stress through increasing total antioxidant capacity (T-AOC) and activities of NADPH dehydrogenase quinone1 (NQO1) and catalase (CAT), and decreasing content of malondialdehyde (MDA). Western blot and qRT-PCR revealed that the expression of nuclear factor erythroid 2-related factor 2 (Nrf2), NQO1 and heme oxygenase 1 (HMOX-1) was increased. Broccoli extracts was better than SF in improving T-AOC, antioxidant enzyme activity (especially, NQO1) and reducing MDA content. Polyphenols and PSF were separated from broccoli extracts by high-speed countercurrent chromatography (HSCCC). They could significantly increase T-AOC and NQO1 activities (P < 0.05). PSF increased activities of Phase 2 enzymes more than polyphenols. Broccoli extracts protected B16 cells from H2O2-induced oxidative stress via enhancing Nrf2/Keap1 signaling pathway. The protection was mainly attributable to SF, followed by polyphenols.Yao ZhangChengzhi LvJuan SunXinjie SongNyasha MakazaYuanfeng WuElsevierarticleBroccoli extractsSulforaphanePolyphenolsOxidative stressNutrition. Foods and food supplyTX341-641ENJournal of Functional Foods, Vol 87, Iss , Pp 104833- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Broccoli extracts Sulforaphane Polyphenols Oxidative stress Nutrition. Foods and food supply TX341-641
spellingShingle	Broccoli extracts Sulforaphane Polyphenols Oxidative stress Nutrition. Foods and food supply TX341-641 Yao Zhang Chengzhi Lv Juan Sun Xinjie Song Nyasha Makaza Yuanfeng Wu Protective effects of broccoli extracts and sulforaphane against hydrogen peroxide induced oxidative stress in B16 cells
description	The antioxidant activity of broccoli extracts and its purified compounds (polyphenols and purified sulforaphane (PSF)) was investigated. Broccoli extracts and SF protected B16 cells from H2O2-induced oxidative stress through increasing total antioxidant capacity (T-AOC) and activities of NADPH dehydrogenase quinone1 (NQO1) and catalase (CAT), and decreasing content of malondialdehyde (MDA). Western blot and qRT-PCR revealed that the expression of nuclear factor erythroid 2-related factor 2 (Nrf2), NQO1 and heme oxygenase 1 (HMOX-1) was increased. Broccoli extracts was better than SF in improving T-AOC, antioxidant enzyme activity (especially, NQO1) and reducing MDA content. Polyphenols and PSF were separated from broccoli extracts by high-speed countercurrent chromatography (HSCCC). They could significantly increase T-AOC and NQO1 activities (P < 0.05). PSF increased activities of Phase 2 enzymes more than polyphenols. Broccoli extracts protected B16 cells from H2O2-induced oxidative stress via enhancing Nrf2/Keap1 signaling pathway. The protection was mainly attributable to SF, followed by polyphenols.
format	article
author	Yao Zhang Chengzhi Lv Juan Sun Xinjie Song Nyasha Makaza Yuanfeng Wu
author_facet	Yao Zhang Chengzhi Lv Juan Sun Xinjie Song Nyasha Makaza Yuanfeng Wu
author_sort	Yao Zhang
title	Protective effects of broccoli extracts and sulforaphane against hydrogen peroxide induced oxidative stress in B16 cells
title_short	Protective effects of broccoli extracts and sulforaphane against hydrogen peroxide induced oxidative stress in B16 cells
title_full	Protective effects of broccoli extracts and sulforaphane against hydrogen peroxide induced oxidative stress in B16 cells
title_fullStr	Protective effects of broccoli extracts and sulforaphane against hydrogen peroxide induced oxidative stress in B16 cells
title_full_unstemmed	Protective effects of broccoli extracts and sulforaphane against hydrogen peroxide induced oxidative stress in B16 cells
title_sort	protective effects of broccoli extracts and sulforaphane against hydrogen peroxide induced oxidative stress in b16 cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/71bb06fefa1342a5bd2fe7818fa504e7
work_keys_str_mv	AT yaozhang protectiveeffectsofbroccoliextractsandsulforaphaneagainsthydrogenperoxideinducedoxidativestressinb16cells AT chengzhilv protectiveeffectsofbroccoliextractsandsulforaphaneagainsthydrogenperoxideinducedoxidativestressinb16cells AT juansun protectiveeffectsofbroccoliextractsandsulforaphaneagainsthydrogenperoxideinducedoxidativestressinb16cells AT xinjiesong protectiveeffectsofbroccoliextractsandsulforaphaneagainsthydrogenperoxideinducedoxidativestressinb16cells AT nyashamakaza protectiveeffectsofbroccoliextractsandsulforaphaneagainsthydrogenperoxideinducedoxidativestressinb16cells AT yuanfengwu protectiveeffectsofbroccoliextractsandsulforaphaneagainsthydrogenperoxideinducedoxidativestressinb16cells
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Protective effects of broccoli extracts and sulforaphane against hydrogen peroxide induced oxidative stress in B16 cells

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