Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province
This study was performed on Ehrlichia canis positive ticks collected from dogs to perform sequencing of their 16S rRNA genetic section using the PCR method. The collection of ticks was performed from a total of 60 dogs in the Siirt province, Turkey. A total of 250 ticks were collected and morphologi...
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oai:doaj.org-article:71bbcb50f27a4832ae8eb485860017762021-11-17T21:27:54ZMolecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province1820-744810.2478/acve-2021-0022https://doaj.org/article/71bbcb50f27a4832ae8eb485860017762021-09-01T00:00:00Zhttps://doi.org/10.2478/acve-2021-0022https://doaj.org/toc/1820-7448This study was performed on Ehrlichia canis positive ticks collected from dogs to perform sequencing of their 16S rRNA genetic section using the PCR method. The collection of ticks was performed from a total of 60 dogs in the Siirt province, Turkey. A total of 250 ticks were collected and morphologically investigated. All ticks were identified as Rhipicephalus sanguineus sensu lato (s.l). Ehrlichial DNA was detected by the PCR method performed on 38 (15.2 %) of the ticks. The E. canis strains obtained as a result of the sequence analysis were found to be 100% identical to the American Texas (MH620194), Indian (KX766395), and Egyptian (MG564254) strains. This study thereby has identified a zoonotic agent from the R. sanguineus ticks collected from the dogs in the Siirt province.Çelik Burçak AslanAyan AdnanYilmaz Ali BilginÇelik Özgür YaşarKilinç Özlem OrunçAyan Özge OktaySciendoarticleehrlichia canisnested pcrrhipicephalus sanguineussiirtturkeyVeterinary medicineSF600-1100ENActa Veterinaria, Vol 71, Iss 3, Pp 247-255 (2021) |
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ehrlichia canis nested pcr rhipicephalus sanguineus siirt turkey Veterinary medicine SF600-1100 |
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ehrlichia canis nested pcr rhipicephalus sanguineus siirt turkey Veterinary medicine SF600-1100 Çelik Burçak Aslan Ayan Adnan Yilmaz Ali Bilgin Çelik Özgür Yaşar Kilinç Özlem Orunç Ayan Özge Oktay Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province |
description |
This study was performed on Ehrlichia canis positive ticks collected from dogs to perform sequencing of their 16S rRNA genetic section using the PCR method. The collection of ticks was performed from a total of 60 dogs in the Siirt province, Turkey. A total of 250 ticks were collected and morphologically investigated. All ticks were identified as Rhipicephalus sanguineus sensu lato (s.l). Ehrlichial DNA was detected by the PCR method performed on 38 (15.2 %) of the ticks. The E. canis strains obtained as a result of the sequence analysis were found to be 100% identical to the American Texas (MH620194), Indian (KX766395), and Egyptian (MG564254) strains. This study thereby has identified a zoonotic agent from the R. sanguineus ticks collected from the dogs in the Siirt province. |
format |
article |
author |
Çelik Burçak Aslan Ayan Adnan Yilmaz Ali Bilgin Çelik Özgür Yaşar Kilinç Özlem Orunç Ayan Özge Oktay |
author_facet |
Çelik Burçak Aslan Ayan Adnan Yilmaz Ali Bilgin Çelik Özgür Yaşar Kilinç Özlem Orunç Ayan Özge Oktay |
author_sort |
Çelik Burçak Aslan |
title |
Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province |
title_short |
Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province |
title_full |
Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province |
title_fullStr |
Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province |
title_full_unstemmed |
Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province |
title_sort |
molecular identification of ehrlichia canis in rhipicephalus sanguineus ticks from siirt province |
publisher |
Sciendo |
publishDate |
2021 |
url |
https://doaj.org/article/71bbcb50f27a4832ae8eb48586001776 |
work_keys_str_mv |
AT celikburcakaslan molecularidentificationofehrlichiacanisinrhipicephalussanguineusticksfromsiirtprovince AT ayanadnan molecularidentificationofehrlichiacanisinrhipicephalussanguineusticksfromsiirtprovince AT yilmazalibilgin molecularidentificationofehrlichiacanisinrhipicephalussanguineusticksfromsiirtprovince AT celikozguryasar molecularidentificationofehrlichiacanisinrhipicephalussanguineusticksfromsiirtprovince AT kilincozlemorunc molecularidentificationofehrlichiacanisinrhipicephalussanguineusticksfromsiirtprovince AT ayanozgeoktay molecularidentificationofehrlichiacanisinrhipicephalussanguineusticksfromsiirtprovince |
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1718425290612408320 |