Comparative RNA-Seq Analysis Reveals Potentially Resistance-Related Genes in Response to Bacterial Canker of Tomato

Tomato is one of the most important crops for human consumption. Its production is affected by the actinomycete Clavibacter michiganensis subsp. michiganensis (Cmm), one of the most devastating bacterial pathogens of this crop. Several wild to...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Leonardo I. Pereyra-Bistraín, Cesaré Ovando-Vázquez, Alejandra Rougon-Cardoso, Ángel G. Alpuche-Solís
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	arcanum bacterial canker <i>Clavibacter</i> gene expression RNA-Seq tomato Genetics QH426-470
Acceso en línea:	https://doaj.org/article/71bc5b57d29b460280ffe3909e583272
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:71bc5b57d29b460280ffe3909e583272
record_format	dspace
spelling	oai:doaj.org-article:71bc5b57d29b460280ffe3909e5832722021-11-25T17:41:28ZComparative RNA-Seq Analysis Reveals Potentially Resistance-Related Genes in Response to Bacterial Canker of Tomato10.3390/genes121117452073-4425https://doaj.org/article/71bc5b57d29b460280ffe3909e5832722021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1745https://doaj.org/toc/2073-4425Tomato is one of the most important crops for human consumption. Its production is affected by the actinomycete <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> (<i>Cmm</i>), one of the most devastating bacterial pathogens of this crop. Several wild tomato species represent a source of natural resistance to <i>Cmm</i>. Here, we contrasted the transcriptomes of the resistant wild tomato species <i>Solanum arcanum</i> LA2157 and the susceptible species <i>Solanum lycopersicum</i> cv. Ailsa Craig, during the first 24 h of challenge with <i>Cmm</i>. We used three analyses approaches which demonstrated to be complementary: mapping to <i>S. lycopersicum</i> reference genome SL3.0; semi de novo transcriptome assembly; and de novo transcriptome assembly. In a global context, transcriptional changes seem to be similar between both species, although there are some specific genes only upregulated in <i>S. arcanum</i> during <i>Cmm</i> interaction, suggesting that the resistance regulatory mechanism probably diverged during the domestication process. Although <i>S. lycopersicum</i> showed enriched functional groups related to defense, <i>S. arcanum</i> displayed a higher number of induced genes related to bacterial, oomycete, and fungal defense at the first few hours of interaction. This study revealed genes that may contribute to the resistance phenotype in the wild tomato species, such as those that encode for a polyphenol oxidase E, diacyl glycerol kinase, TOM1-like protein 6, and an ankyrin repeat-containing protein, among others. This work will contribute to a better understanding of the defense mechanism against <i>Cmm</i>, and the development of new control methods.Leonardo I. Pereyra-BistraínCesaré Ovando-VázquezAlejandra Rougon-CardosoÁngel G. Alpuche-SolísMDPI AGarticlearcanumbacterial canker<i>Clavibacter</i>gene expressionRNA-SeqtomatoGeneticsQH426-470ENGenes, Vol 12, Iss 1745, p 1745 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	arcanum bacterial canker <i>Clavibacter</i> gene expression RNA-Seq tomato Genetics QH426-470
spellingShingle	arcanum bacterial canker <i>Clavibacter</i> gene expression RNA-Seq tomato Genetics QH426-470 Leonardo I. Pereyra-Bistraín Cesaré Ovando-Vázquez Alejandra Rougon-Cardoso Ángel G. Alpuche-Solís Comparative RNA-Seq Analysis Reveals Potentially Resistance-Related Genes in Response to Bacterial Canker of Tomato
description	Tomato is one of the most important crops for human consumption. Its production is affected by the actinomycete <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> (<i>Cmm</i>), one of the most devastating bacterial pathogens of this crop. Several wild tomato species represent a source of natural resistance to <i>Cmm</i>. Here, we contrasted the transcriptomes of the resistant wild tomato species <i>Solanum arcanum</i> LA2157 and the susceptible species <i>Solanum lycopersicum</i> cv. Ailsa Craig, during the first 24 h of challenge with <i>Cmm</i>. We used three analyses approaches which demonstrated to be complementary: mapping to <i>S. lycopersicum</i> reference genome SL3.0; semi de novo transcriptome assembly; and de novo transcriptome assembly. In a global context, transcriptional changes seem to be similar between both species, although there are some specific genes only upregulated in <i>S. arcanum</i> during <i>Cmm</i> interaction, suggesting that the resistance regulatory mechanism probably diverged during the domestication process. Although <i>S. lycopersicum</i> showed enriched functional groups related to defense, <i>S. arcanum</i> displayed a higher number of induced genes related to bacterial, oomycete, and fungal defense at the first few hours of interaction. This study revealed genes that may contribute to the resistance phenotype in the wild tomato species, such as those that encode for a polyphenol oxidase E, diacyl glycerol kinase, TOM1-like protein 6, and an ankyrin repeat-containing protein, among others. This work will contribute to a better understanding of the defense mechanism against <i>Cmm</i>, and the development of new control methods.
format	article
author	Leonardo I. Pereyra-Bistraín Cesaré Ovando-Vázquez Alejandra Rougon-Cardoso Ángel G. Alpuche-Solís
author_facet	Leonardo I. Pereyra-Bistraín Cesaré Ovando-Vázquez Alejandra Rougon-Cardoso Ángel G. Alpuche-Solís
author_sort	Leonardo I. Pereyra-Bistraín
title	Comparative RNA-Seq Analysis Reveals Potentially Resistance-Related Genes in Response to Bacterial Canker of Tomato
title_short	Comparative RNA-Seq Analysis Reveals Potentially Resistance-Related Genes in Response to Bacterial Canker of Tomato
title_full	Comparative RNA-Seq Analysis Reveals Potentially Resistance-Related Genes in Response to Bacterial Canker of Tomato
title_fullStr	Comparative RNA-Seq Analysis Reveals Potentially Resistance-Related Genes in Response to Bacterial Canker of Tomato
title_full_unstemmed	Comparative RNA-Seq Analysis Reveals Potentially Resistance-Related Genes in Response to Bacterial Canker of Tomato
title_sort	comparative rna-seq analysis reveals potentially resistance-related genes in response to bacterial canker of tomato
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/71bc5b57d29b460280ffe3909e583272
work_keys_str_mv	AT leonardoipereyrabistrain comparativernaseqanalysisrevealspotentiallyresistancerelatedgenesinresponsetobacterialcankeroftomato AT cesareovandovazquez comparativernaseqanalysisrevealspotentiallyresistancerelatedgenesinresponsetobacterialcankeroftomato AT alejandrarougoncardoso comparativernaseqanalysisrevealspotentiallyresistancerelatedgenesinresponsetobacterialcankeroftomato AT angelgalpuchesolis comparativernaseqanalysisrevealspotentiallyresistancerelatedgenesinresponsetobacterialcankeroftomato
_version_	1718412110780694528

Comparative RNA-Seq Analysis Reveals Potentially Resistance-Related Genes in Response to Bacterial Canker of Tomato

Ejemplares similares