High cholesterol diet induces IL-1β expression in adult but not larval zebrafish.

Recently, it has been demonstrated that high cholesterol diet induced hypercholesterolemia and vascular lipid oxidation and accumulation in zebrafish larvae, suggesting that zebrafish is a new promising atherosclerosis model in addition to mouse models. However, up to date, there was no report regar...

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Autores principales:	Yina Yoon, Jihye Yoon, Man-Young Jang, Yirang Na, Youngho Ko, Jae-Hoon Choi, Seung Hyeok Seok
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/71c86064b2d44c7287f1f0f0de25d199
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spelling	oai:doaj.org-article:71c86064b2d44c7287f1f0f0de25d1992021-11-18T07:40:07ZHigh cholesterol diet induces IL-1β expression in adult but not larval zebrafish.1932-620310.1371/journal.pone.0066970https://doaj.org/article/71c86064b2d44c7287f1f0f0de25d1992013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23825600/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Recently, it has been demonstrated that high cholesterol diet induced hypercholesterolemia and vascular lipid oxidation and accumulation in zebrafish larvae, suggesting that zebrafish is a new promising atherosclerosis model in addition to mouse models. However, up to date, there was no report regarding inflammatory cytokine expression during the lipid accumulation in zebrafish larva and adult fish. In this study, we first demonstrated the expression levels of IL-1β and TNF-α in high cholesterol diet (HCD)-fed zebrafish larvae, and found that although HCD induced vascular lipid accumulation, the cytokine expressions in the larvae were not changed by HCD. Furthermore, there was no significant difference in leukocyte accumulation in vessels between control and HCD fed group. But prolonged HCD induced IL-1β expression in spleen and liver compared to those of control zebrafish, and produced very early stage of fatty streak lesion in dorsal aorta of 19 week HCD-fed zebrafish. These results indicate that HCD induced hypercholesterolemia and atherosclerotic changes in zebrafish are very early stage, and suggest the necessity of the generation of mutant zebrafish having a disruption in a lipid metabolism-related gene leading to severe hypercholesterolemia and advanced atherosclerosis.Yina YoonJihye YoonMan-Young JangYirang NaYoungho KoJae-Hoon ChoiSeung Hyeok SeokPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e66970 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yina Yoon Jihye Yoon Man-Young Jang Yirang Na Youngho Ko Jae-Hoon Choi Seung Hyeok Seok High cholesterol diet induces IL-1β expression in adult but not larval zebrafish.
description	Recently, it has been demonstrated that high cholesterol diet induced hypercholesterolemia and vascular lipid oxidation and accumulation in zebrafish larvae, suggesting that zebrafish is a new promising atherosclerosis model in addition to mouse models. However, up to date, there was no report regarding inflammatory cytokine expression during the lipid accumulation in zebrafish larva and adult fish. In this study, we first demonstrated the expression levels of IL-1β and TNF-α in high cholesterol diet (HCD)-fed zebrafish larvae, and found that although HCD induced vascular lipid accumulation, the cytokine expressions in the larvae were not changed by HCD. Furthermore, there was no significant difference in leukocyte accumulation in vessels between control and HCD fed group. But prolonged HCD induced IL-1β expression in spleen and liver compared to those of control zebrafish, and produced very early stage of fatty streak lesion in dorsal aorta of 19 week HCD-fed zebrafish. These results indicate that HCD induced hypercholesterolemia and atherosclerotic changes in zebrafish are very early stage, and suggest the necessity of the generation of mutant zebrafish having a disruption in a lipid metabolism-related gene leading to severe hypercholesterolemia and advanced atherosclerosis.
format	article
author	Yina Yoon Jihye Yoon Man-Young Jang Yirang Na Youngho Ko Jae-Hoon Choi Seung Hyeok Seok
author_facet	Yina Yoon Jihye Yoon Man-Young Jang Yirang Na Youngho Ko Jae-Hoon Choi Seung Hyeok Seok
author_sort	Yina Yoon
title	High cholesterol diet induces IL-1β expression in adult but not larval zebrafish.
title_short	High cholesterol diet induces IL-1β expression in adult but not larval zebrafish.
title_full	High cholesterol diet induces IL-1β expression in adult but not larval zebrafish.
title_fullStr	High cholesterol diet induces IL-1β expression in adult but not larval zebrafish.
title_full_unstemmed	High cholesterol diet induces IL-1β expression in adult but not larval zebrafish.
title_sort	high cholesterol diet induces il-1β expression in adult but not larval zebrafish.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/71c86064b2d44c7287f1f0f0de25d199
work_keys_str_mv	AT yinayoon highcholesteroldietinducesil1bexpressioninadultbutnotlarvalzebrafish AT jihyeyoon highcholesteroldietinducesil1bexpressioninadultbutnotlarvalzebrafish AT manyoungjang highcholesteroldietinducesil1bexpressioninadultbutnotlarvalzebrafish AT yirangna highcholesteroldietinducesil1bexpressioninadultbutnotlarvalzebrafish AT younghoko highcholesteroldietinducesil1bexpressioninadultbutnotlarvalzebrafish AT jaehoonchoi highcholesteroldietinducesil1bexpressioninadultbutnotlarvalzebrafish AT seunghyeokseok highcholesteroldietinducesil1bexpressioninadultbutnotlarvalzebrafish
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High cholesterol diet induces IL-1β expression in adult but not larval zebrafish.

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