Apple Polyphenols Extract (APE) Alleviated Dextran Sulfate Sodium Induced Acute Ulcerative Colitis and Accompanying Neuroinflammation via Inhibition of Apoptosis and Pyroptosis

The main aim of this study was to investigate the potent anti-apoptosis and anti-pyroptosis effects of apple polyphenols extract (APE) on dextran sulfate sodium model group (DSS)-induced acute ulcerative colitis (UC) and the protective effect of APE against acute UC-related neuroinflammation and syn...

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Autores principales:	Fang Liu, Xinjing Wang, Yuan Cui, Yan Yin, Dong Qiu, Shilan Li, Xinli Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	apple polyphenols extracts ulcerative colitis apoptosis pyroptosis neuroinflammation Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/71d44d10d98047d9ae6afce9cbdbec7a
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spelling	oai:doaj.org-article:71d44d10d98047d9ae6afce9cbdbec7a2021-11-25T17:34:28ZApple Polyphenols Extract (APE) Alleviated Dextran Sulfate Sodium Induced Acute Ulcerative Colitis and Accompanying Neuroinflammation via Inhibition of Apoptosis and Pyroptosis10.3390/foods101127112304-8158https://doaj.org/article/71d44d10d98047d9ae6afce9cbdbec7a2021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2711https://doaj.org/toc/2304-8158The main aim of this study was to investigate the potent anti-apoptosis and anti-pyroptosis effects of apple polyphenols extract (APE) on dextran sulfate sodium model group (DSS)-induced acute ulcerative colitis (UC) and the protective effect of APE against acute UC-related neuroinflammation and synapse damage. Forty-three C57BL/6 male mice were randomly divided into a control group (CON), a 3% DSS model group (DSS), a 500 mg/(kg·bw·d) APE group (HAP), and a 125 (LD) or 500 (HD) mg/(kg·bw·d) APE treatment concomitantly with DSS treatment group. The results showed that APE significantly ameliorated DSS-induced acute UC through inhibiting intestinal epithelial cell (IEC) apoptosis and the Caspase-1/Caspase-11-dependent pyroptosis pathway, with increased BCL-2 protein expression and decreased protein levels of NLRP3, ASC, Caspase-1/11, and GSDND. Furthermore, APE significantly reduced acute UC-related neuroinflammation and synapse damage, supported by decreased mRNA levels of hypothalamus <i>Cox-2</i> and hippocampus <i>Gfap</i> and also increased the mRNA levels of hypothalamus <i>Psd-95</i>. The increased protein expression of ZO-1 and Occludin improved the intestinal barrier integrity and improved the function of goblet cells by upregulating the protein level of MUC-2 and TTF3 accounted for the beneficial effects of APE on UC-associated neuroinflammation. Therefore, APE might be a safe and effective agent for the management of acute UC.Fang LiuXinjing WangYuan CuiYan YinDong QiuShilan LiXinli LiMDPI AGarticleapple polyphenols extractsulcerative colitisapoptosispyroptosisneuroinflammationChemical technologyTP1-1185ENFoods, Vol 10, Iss 2711, p 2711 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	apple polyphenols extracts ulcerative colitis apoptosis pyroptosis neuroinflammation Chemical technology TP1-1185
spellingShingle	apple polyphenols extracts ulcerative colitis apoptosis pyroptosis neuroinflammation Chemical technology TP1-1185 Fang Liu Xinjing Wang Yuan Cui Yan Yin Dong Qiu Shilan Li Xinli Li Apple Polyphenols Extract (APE) Alleviated Dextran Sulfate Sodium Induced Acute Ulcerative Colitis and Accompanying Neuroinflammation via Inhibition of Apoptosis and Pyroptosis
description	The main aim of this study was to investigate the potent anti-apoptosis and anti-pyroptosis effects of apple polyphenols extract (APE) on dextran sulfate sodium model group (DSS)-induced acute ulcerative colitis (UC) and the protective effect of APE against acute UC-related neuroinflammation and synapse damage. Forty-three C57BL/6 male mice were randomly divided into a control group (CON), a 3% DSS model group (DSS), a 500 mg/(kg·bw·d) APE group (HAP), and a 125 (LD) or 500 (HD) mg/(kg·bw·d) APE treatment concomitantly with DSS treatment group. The results showed that APE significantly ameliorated DSS-induced acute UC through inhibiting intestinal epithelial cell (IEC) apoptosis and the Caspase-1/Caspase-11-dependent pyroptosis pathway, with increased BCL-2 protein expression and decreased protein levels of NLRP3, ASC, Caspase-1/11, and GSDND. Furthermore, APE significantly reduced acute UC-related neuroinflammation and synapse damage, supported by decreased mRNA levels of hypothalamus <i>Cox-2</i> and hippocampus <i>Gfap</i> and also increased the mRNA levels of hypothalamus <i>Psd-95</i>. The increased protein expression of ZO-1 and Occludin improved the intestinal barrier integrity and improved the function of goblet cells by upregulating the protein level of MUC-2 and TTF3 accounted for the beneficial effects of APE on UC-associated neuroinflammation. Therefore, APE might be a safe and effective agent for the management of acute UC.
format	article
author	Fang Liu Xinjing Wang Yuan Cui Yan Yin Dong Qiu Shilan Li Xinli Li
author_facet	Fang Liu Xinjing Wang Yuan Cui Yan Yin Dong Qiu Shilan Li Xinli Li
author_sort	Fang Liu
title	Apple Polyphenols Extract (APE) Alleviated Dextran Sulfate Sodium Induced Acute Ulcerative Colitis and Accompanying Neuroinflammation via Inhibition of Apoptosis and Pyroptosis
title_short	Apple Polyphenols Extract (APE) Alleviated Dextran Sulfate Sodium Induced Acute Ulcerative Colitis and Accompanying Neuroinflammation via Inhibition of Apoptosis and Pyroptosis
title_full	Apple Polyphenols Extract (APE) Alleviated Dextran Sulfate Sodium Induced Acute Ulcerative Colitis and Accompanying Neuroinflammation via Inhibition of Apoptosis and Pyroptosis
title_fullStr	Apple Polyphenols Extract (APE) Alleviated Dextran Sulfate Sodium Induced Acute Ulcerative Colitis and Accompanying Neuroinflammation via Inhibition of Apoptosis and Pyroptosis
title_full_unstemmed	Apple Polyphenols Extract (APE) Alleviated Dextran Sulfate Sodium Induced Acute Ulcerative Colitis and Accompanying Neuroinflammation via Inhibition of Apoptosis and Pyroptosis
title_sort	apple polyphenols extract (ape) alleviated dextran sulfate sodium induced acute ulcerative colitis and accompanying neuroinflammation via inhibition of apoptosis and pyroptosis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/71d44d10d98047d9ae6afce9cbdbec7a
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Apple Polyphenols Extract (APE) Alleviated Dextran Sulfate Sodium Induced Acute Ulcerative Colitis and Accompanying Neuroinflammation via Inhibition of Apoptosis and Pyroptosis

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