Nanoparticles of conformation-stabilized canine distemper virus hemagglutinin are highly immunogenic and induce robust immunity

Abstract Background Canine distemper virus (CDV) infection of ferrets, dogs, and giant pandas causes an acute systemic disease involving multiple organ systems, including the respiratory tract, lymphoid system, and central nervous system. In this study, we tested a new candidate CDV vaccine-CDV nano...

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Autores principales:	Jingjian Dong, Yan Chen, Lili Shi, Bing Shen, Xianliang Sun, Kaiyi Ruan, Xianzhu Xia, Hao Feng, Na Feng
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Canine distemper virus Nanoparticle Mucosal immune response Hemagglutinin (H) tetramer Giant panda Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/71d91e9fb6fc4243a776a010bfa26fb5
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spelling	oai:doaj.org-article:71d91e9fb6fc4243a776a010bfa26fb52021-11-28T12:03:06ZNanoparticles of conformation-stabilized canine distemper virus hemagglutinin are highly immunogenic and induce robust immunity10.1186/s12985-021-01702-01743-422Xhttps://doaj.org/article/71d91e9fb6fc4243a776a010bfa26fb52021-11-01T00:00:00Zhttps://doi.org/10.1186/s12985-021-01702-0https://doaj.org/toc/1743-422XAbstract Background Canine distemper virus (CDV) infection of ferrets, dogs, and giant pandas causes an acute systemic disease involving multiple organ systems, including the respiratory tract, lymphoid system, and central nervous system. In this study, we tested a new candidate CDV vaccine-CDV nanoparticles-based on hemagglutinin protein. Methods The nanoparticles were generated from conformation-stabilized CDV hemagglutinin tetramers. Immune responses against CDV were evaluated in mice. Immunization was initiated 6 weeks after birth and boosted two times with 4-week intervals. The blood and mucosal samples were collected 2 weeks after each immunization. Results Vaccination with CDV nanoparticles elicited high levels of IgG antibody titers in mice (approximately sevenfold to eightfold higher than that obtained with soluble CDV H protein) and mucosal immune responses and developed increased CDV-specific neutralizing antibody. The mice that received nanoparticles showed significantly higher IFN-γ- and IL-4-secreting cell population in the spleen and lymph node compared with mice immunized with soluble H protein. The co-stimulatory molecular expression of CD80 and CD86 on the surface of DCs was also upregulated. Conclusion The results demonstrate that self-assembly into nanoparticles can increase the immunogenicity of vaccine antigens, and nanoparticles assembled from conformation-stabilized CDV H protein can serve as a new CDV vaccine.Jingjian DongYan ChenLili ShiBing ShenXianliang SunKaiyi RuanXianzhu XiaHao FengNa FengBMCarticleCanine distemper virusNanoparticleMucosal immune responseHemagglutinin (H) tetramerGiant pandaInfectious and parasitic diseasesRC109-216ENVirology Journal, Vol 18, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Canine distemper virus Nanoparticle Mucosal immune response Hemagglutinin (H) tetramer Giant panda Infectious and parasitic diseases RC109-216
spellingShingle	Canine distemper virus Nanoparticle Mucosal immune response Hemagglutinin (H) tetramer Giant panda Infectious and parasitic diseases RC109-216 Jingjian Dong Yan Chen Lili Shi Bing Shen Xianliang Sun Kaiyi Ruan Xianzhu Xia Hao Feng Na Feng Nanoparticles of conformation-stabilized canine distemper virus hemagglutinin are highly immunogenic and induce robust immunity
description	Abstract Background Canine distemper virus (CDV) infection of ferrets, dogs, and giant pandas causes an acute systemic disease involving multiple organ systems, including the respiratory tract, lymphoid system, and central nervous system. In this study, we tested a new candidate CDV vaccine-CDV nanoparticles-based on hemagglutinin protein. Methods The nanoparticles were generated from conformation-stabilized CDV hemagglutinin tetramers. Immune responses against CDV were evaluated in mice. Immunization was initiated 6 weeks after birth and boosted two times with 4-week intervals. The blood and mucosal samples were collected 2 weeks after each immunization. Results Vaccination with CDV nanoparticles elicited high levels of IgG antibody titers in mice (approximately sevenfold to eightfold higher than that obtained with soluble CDV H protein) and mucosal immune responses and developed increased CDV-specific neutralizing antibody. The mice that received nanoparticles showed significantly higher IFN-γ- and IL-4-secreting cell population in the spleen and lymph node compared with mice immunized with soluble H protein. The co-stimulatory molecular expression of CD80 and CD86 on the surface of DCs was also upregulated. Conclusion The results demonstrate that self-assembly into nanoparticles can increase the immunogenicity of vaccine antigens, and nanoparticles assembled from conformation-stabilized CDV H protein can serve as a new CDV vaccine.
format	article
author	Jingjian Dong Yan Chen Lili Shi Bing Shen Xianliang Sun Kaiyi Ruan Xianzhu Xia Hao Feng Na Feng
author_facet	Jingjian Dong Yan Chen Lili Shi Bing Shen Xianliang Sun Kaiyi Ruan Xianzhu Xia Hao Feng Na Feng
author_sort	Jingjian Dong
title	Nanoparticles of conformation-stabilized canine distemper virus hemagglutinin are highly immunogenic and induce robust immunity
title_short	Nanoparticles of conformation-stabilized canine distemper virus hemagglutinin are highly immunogenic and induce robust immunity
title_full	Nanoparticles of conformation-stabilized canine distemper virus hemagglutinin are highly immunogenic and induce robust immunity
title_fullStr	Nanoparticles of conformation-stabilized canine distemper virus hemagglutinin are highly immunogenic and induce robust immunity
title_full_unstemmed	Nanoparticles of conformation-stabilized canine distemper virus hemagglutinin are highly immunogenic and induce robust immunity
title_sort	nanoparticles of conformation-stabilized canine distemper virus hemagglutinin are highly immunogenic and induce robust immunity
publisher	BMC
publishDate	2021
url	https://doaj.org/article/71d91e9fb6fc4243a776a010bfa26fb5
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Nanoparticles of conformation-stabilized canine distemper virus hemagglutinin are highly immunogenic and induce robust immunity

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