Preparation And Evaluation Of A Recent Infectious Laryngotracheitis (Ilt) Vaccine From A Local Field Isolate

Infectious laryngotracheitis (ILT) is a respiratory tract disease affecting chickens worldwide. The disease leads to severe production losses due to increased mortality, decreased egg production, and delayed body weight gain, causing enormous economic losses to the poultry industry. ILT is controlle...

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Autores principales:	Nada A. Fathy, Abd El-Moneam M.M., Ibrahim S., Norhan Nagy Mohamed, Amina A. Radwan, Rafik Hamed Sayed
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2020
Materias:	bvdv ilt ceo tco spf elisa Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2020.101927 https://doaj.org/article/71d980f36b0643ac94fb627ddd3c025e
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spelling	oai:doaj.org-article:71d980f36b0643ac94fb627ddd3c025e2021-12-02T13:24:02ZPreparation And Evaluation Of A Recent Infectious Laryngotracheitis (Ilt) Vaccine From A Local Field Isolatehttps://dx.doi.org/10.21608/javs.2020.1019271687-40722090-3308https://doaj.org/article/71d980f36b0643ac94fb627ddd3c025e2020-07-01T00:00:00Zhttps://javs.journals.ekb.eg/article_101927.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Infectious laryngotracheitis (ILT) is a respiratory tract disease affecting chickens worldwide. The disease leads to severe production losses due to increased mortality, decreased egg production, and delayed body weight gain, causing enormous economic losses to the poultry industry. ILT is controlled through vaccination with live-modified attenuated vaccines of chicken embryo origin (CEO) and/or the tissue-culture origin (TCO). This study was conducted to develop Specific Pathogenic Free (SPF) egg-adapted live attenuated ILT vaccine from the field strain Fayoum_2018 isolated from broilers in Fayoum Province, Egypt in 2018. The isolate Fayoum_2018 with accession number (MN082684) was characterized molecularly as a TCO vaccine related strain. Measurement of immunity in vaccinated chicks employing the potency test revealed that chicks vaccinated with 0.5 ml of prepared ILT vaccine exhibited ELISA with 1905 antibody titer at 2nd-week post-vaccination, which was considered an ILT adequate immune response and increased till reach 3497 antibody titer at 6th week post-vaccination. The current work revealed that Fayoum_2018 ILTV egg adapted live attenuated vaccine produced a satisfactory antibodies titer that was efficient in controlling the ILT field infection in Egypt.Nada A. FathyAbd El-Moneam M.M.Ibrahim S.Norhan Nagy MohamedAmina A. RadwanRafik Hamed SayedEgyptian Society for Animal ManagementarticlebvdviltceotcospfelisaZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 5, Iss 3, Pp 87-91 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bvdv ilt ceo tco spf elisa Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	bvdv ilt ceo tco spf elisa Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Nada A. Fathy Abd El-Moneam M.M. Ibrahim S. Norhan Nagy Mohamed Amina A. Radwan Rafik Hamed Sayed Preparation And Evaluation Of A Recent Infectious Laryngotracheitis (Ilt) Vaccine From A Local Field Isolate
description	Infectious laryngotracheitis (ILT) is a respiratory tract disease affecting chickens worldwide. The disease leads to severe production losses due to increased mortality, decreased egg production, and delayed body weight gain, causing enormous economic losses to the poultry industry. ILT is controlled through vaccination with live-modified attenuated vaccines of chicken embryo origin (CEO) and/or the tissue-culture origin (TCO). This study was conducted to develop Specific Pathogenic Free (SPF) egg-adapted live attenuated ILT vaccine from the field strain Fayoum_2018 isolated from broilers in Fayoum Province, Egypt in 2018. The isolate Fayoum_2018 with accession number (MN082684) was characterized molecularly as a TCO vaccine related strain. Measurement of immunity in vaccinated chicks employing the potency test revealed that chicks vaccinated with 0.5 ml of prepared ILT vaccine exhibited ELISA with 1905 antibody titer at 2nd-week post-vaccination, which was considered an ILT adequate immune response and increased till reach 3497 antibody titer at 6th week post-vaccination. The current work revealed that Fayoum_2018 ILTV egg adapted live attenuated vaccine produced a satisfactory antibodies titer that was efficient in controlling the ILT field infection in Egypt.
format	article
author	Nada A. Fathy Abd El-Moneam M.M. Ibrahim S. Norhan Nagy Mohamed Amina A. Radwan Rafik Hamed Sayed
author_facet	Nada A. Fathy Abd El-Moneam M.M. Ibrahim S. Norhan Nagy Mohamed Amina A. Radwan Rafik Hamed Sayed
author_sort	Nada A. Fathy
title	Preparation And Evaluation Of A Recent Infectious Laryngotracheitis (Ilt) Vaccine From A Local Field Isolate
title_short	Preparation And Evaluation Of A Recent Infectious Laryngotracheitis (Ilt) Vaccine From A Local Field Isolate
title_full	Preparation And Evaluation Of A Recent Infectious Laryngotracheitis (Ilt) Vaccine From A Local Field Isolate
title_fullStr	Preparation And Evaluation Of A Recent Infectious Laryngotracheitis (Ilt) Vaccine From A Local Field Isolate
title_full_unstemmed	Preparation And Evaluation Of A Recent Infectious Laryngotracheitis (Ilt) Vaccine From A Local Field Isolate
title_sort	preparation and evaluation of a recent infectious laryngotracheitis (ilt) vaccine from a local field isolate
publisher	Egyptian Society for Animal Management
publishDate	2020
url	https://dx.doi.org/10.21608/javs.2020.101927 https://doaj.org/article/71d980f36b0643ac94fb627ddd3c025e
work_keys_str_mv	AT nadaafathy preparationandevaluationofarecentinfectiouslaryngotracheitisiltvaccinefromalocalfieldisolate AT abdelmoneammm preparationandevaluationofarecentinfectiouslaryngotracheitisiltvaccinefromalocalfieldisolate AT ibrahims preparationandevaluationofarecentinfectiouslaryngotracheitisiltvaccinefromalocalfieldisolate AT norhannagymohamed preparationandevaluationofarecentinfectiouslaryngotracheitisiltvaccinefromalocalfieldisolate AT aminaaradwan preparationandevaluationofarecentinfectiouslaryngotracheitisiltvaccinefromalocalfieldisolate AT rafikhamedsayed preparationandevaluationofarecentinfectiouslaryngotracheitisiltvaccinefromalocalfieldisolate
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Preparation And Evaluation Of A Recent Infectious Laryngotracheitis (Ilt) Vaccine From A Local Field Isolate

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