Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.

Bifidobacteria are claimed to contribute positively to human health through a range of beneficial or probiotic activities, including amelioration of gastrointestinal and metabolic disorders, and therefore this particular group of gastrointestinal commensals has enjoyed increasing industrial and scie...

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Autores principales:	Lorena Ruiz, Mary O'Connell Motherway, Noreen Lanigan, Douwe van Sinderen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/71e428cacd024ef293085296df454c12
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spelling	oai:doaj.org-article:71e428cacd024ef293085296df454c122021-11-18T07:43:47ZTransposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.1932-620310.1371/journal.pone.0064699https://doaj.org/article/71e428cacd024ef293085296df454c122013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23737995/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Bifidobacteria are claimed to contribute positively to human health through a range of beneficial or probiotic activities, including amelioration of gastrointestinal and metabolic disorders, and therefore this particular group of gastrointestinal commensals has enjoyed increasing industrial and scientific attention in recent years. However, the molecular mechanisms underlying these probiotic mechanisms are still largely unknown, mainly due to the fact that molecular tools for bifidobacteria are rather poorly developed, with many strains lacking genetic accessibility. In this work, we describe the generation of transposon insertion mutants in two bifidobacterial strains, B. breve UCC2003 and B. breve NCFB2258. We also report the creation of the first transposon mutant library in a bifidobacterial strain, employing B. breve UCC2003 and a Tn5-based transposome strategy. The library was found to be composed of clones containing single transposon insertions which appear to be randomly distributed along the genome. The usefulness of the library to perform phenotypic screenings was confirmed through identification and analysis of mutants defective in D-galactose, D-lactose or pullulan utilization abilities.Lorena RuizMary O'Connell MotherwayNoreen LaniganDouwe van SinderenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e64699 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lorena Ruiz Mary O'Connell Motherway Noreen Lanigan Douwe van Sinderen Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.
description	Bifidobacteria are claimed to contribute positively to human health through a range of beneficial or probiotic activities, including amelioration of gastrointestinal and metabolic disorders, and therefore this particular group of gastrointestinal commensals has enjoyed increasing industrial and scientific attention in recent years. However, the molecular mechanisms underlying these probiotic mechanisms are still largely unknown, mainly due to the fact that molecular tools for bifidobacteria are rather poorly developed, with many strains lacking genetic accessibility. In this work, we describe the generation of transposon insertion mutants in two bifidobacterial strains, B. breve UCC2003 and B. breve NCFB2258. We also report the creation of the first transposon mutant library in a bifidobacterial strain, employing B. breve UCC2003 and a Tn5-based transposome strategy. The library was found to be composed of clones containing single transposon insertions which appear to be randomly distributed along the genome. The usefulness of the library to perform phenotypic screenings was confirmed through identification and analysis of mutants defective in D-galactose, D-lactose or pullulan utilization abilities.
format	article
author	Lorena Ruiz Mary O'Connell Motherway Noreen Lanigan Douwe van Sinderen
author_facet	Lorena Ruiz Mary O'Connell Motherway Noreen Lanigan Douwe van Sinderen
author_sort	Lorena Ruiz
title	Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.
title_short	Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.
title_full	Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.
title_fullStr	Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.
title_full_unstemmed	Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.
title_sort	transposon mutagenesis in bifidobacterium breve: construction and characterization of a tn5 transposon mutant library for bifidobacterium breve ucc2003.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/71e428cacd024ef293085296df454c12
work_keys_str_mv	AT lorenaruiz transposonmutagenesisinbifidobacteriumbreveconstructionandcharacterizationofatn5transposonmutantlibraryforbifidobacteriumbreveucc2003 AT maryoconnellmotherway transposonmutagenesisinbifidobacteriumbreveconstructionandcharacterizationofatn5transposonmutantlibraryforbifidobacteriumbreveucc2003 AT noreenlanigan transposonmutagenesisinbifidobacteriumbreveconstructionandcharacterizationofatn5transposonmutantlibraryforbifidobacteriumbreveucc2003 AT douwevansinderen transposonmutagenesisinbifidobacteriumbreveconstructionandcharacterizationofatn5transposonmutantlibraryforbifidobacteriumbreveucc2003
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Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.

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