Mobile nucleic acid amplification testing (mobiNAAT) for Chlamydia trachomatis screening in hospital emergency department settings

Abstract Management of curable sexually-transmitted infections (STI) such as Chlamydia can be revolutionized by highly sensitive nucleic acid testing that is deployable at the point-of-care (POC). Here we report the development of a mobile nucleic acid amplification testing (mobiNAAT) platform utili...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	D. J. Shin, P. Athamanolap, L. Chen, J. Hardick, M. Lewis, Y. H. Hsieh, R. E. Rothman, C. A. Gaydos, T. H. Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/71e671c7a77547ab9c88c20675696df8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:71e671c7a77547ab9c88c20675696df8
record_format	dspace
spelling	oai:doaj.org-article:71e671c7a77547ab9c88c20675696df82021-12-02T11:52:31ZMobile nucleic acid amplification testing (mobiNAAT) for Chlamydia trachomatis screening in hospital emergency department settings10.1038/s41598-017-04781-82045-2322https://doaj.org/article/71e671c7a77547ab9c88c20675696df82017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04781-8https://doaj.org/toc/2045-2322Abstract Management of curable sexually-transmitted infections (STI) such as Chlamydia can be revolutionized by highly sensitive nucleic acid testing that is deployable at the point-of-care (POC). Here we report the development of a mobile nucleic acid amplification testing (mobiNAAT) platform utilizing a mobile phone and droplet magnetofluidics to deliver NAAT in a portable and accessible format. By using magnetic particles as a mobile substrate for nucleic acid capture and transport, fluid handling is reduced to particle translocation on a simple magnetofluidic cartridge assembled with reagents for nucleic acid purification and amplification. A mobile phone user interface operating in tandem with a portable Bluetooth-enabled cartridge-processing unit facilitates process integration. We tested 30 potentially Chlamydia trachomatis (CT)-infected patients in a hospital emergency department and confirmed that mobiNAAT showed 100% concordance with laboratory-based NAAT. Concurrent evaluation by a nontechnical study coordinator who received brief training via an embedded mobile app module demonstrated ease of use and reproducibility of the platform. This work demonstrates the potential of mobile nucleic acid testing in bridging the diagnostic gap between centralized laboratories and hospital emergency departments.D. J. ShinP. AthamanolapL. ChenJ. HardickM. LewisY. H. HsiehR. E. RothmanC. A. GaydosT. H. WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q D. J. Shin P. Athamanolap L. Chen J. Hardick M. Lewis Y. H. Hsieh R. E. Rothman C. A. Gaydos T. H. Wang Mobile nucleic acid amplification testing (mobiNAAT) for Chlamydia trachomatis screening in hospital emergency department settings
description	Abstract Management of curable sexually-transmitted infections (STI) such as Chlamydia can be revolutionized by highly sensitive nucleic acid testing that is deployable at the point-of-care (POC). Here we report the development of a mobile nucleic acid amplification testing (mobiNAAT) platform utilizing a mobile phone and droplet magnetofluidics to deliver NAAT in a portable and accessible format. By using magnetic particles as a mobile substrate for nucleic acid capture and transport, fluid handling is reduced to particle translocation on a simple magnetofluidic cartridge assembled with reagents for nucleic acid purification and amplification. A mobile phone user interface operating in tandem with a portable Bluetooth-enabled cartridge-processing unit facilitates process integration. We tested 30 potentially Chlamydia trachomatis (CT)-infected patients in a hospital emergency department and confirmed that mobiNAAT showed 100% concordance with laboratory-based NAAT. Concurrent evaluation by a nontechnical study coordinator who received brief training via an embedded mobile app module demonstrated ease of use and reproducibility of the platform. This work demonstrates the potential of mobile nucleic acid testing in bridging the diagnostic gap between centralized laboratories and hospital emergency departments.
format	article
author	D. J. Shin P. Athamanolap L. Chen J. Hardick M. Lewis Y. H. Hsieh R. E. Rothman C. A. Gaydos T. H. Wang
author_facet	D. J. Shin P. Athamanolap L. Chen J. Hardick M. Lewis Y. H. Hsieh R. E. Rothman C. A. Gaydos T. H. Wang
author_sort	D. J. Shin
title	Mobile nucleic acid amplification testing (mobiNAAT) for Chlamydia trachomatis screening in hospital emergency department settings
title_short	Mobile nucleic acid amplification testing (mobiNAAT) for Chlamydia trachomatis screening in hospital emergency department settings
title_full	Mobile nucleic acid amplification testing (mobiNAAT) for Chlamydia trachomatis screening in hospital emergency department settings
title_fullStr	Mobile nucleic acid amplification testing (mobiNAAT) for Chlamydia trachomatis screening in hospital emergency department settings
title_full_unstemmed	Mobile nucleic acid amplification testing (mobiNAAT) for Chlamydia trachomatis screening in hospital emergency department settings
title_sort	mobile nucleic acid amplification testing (mobinaat) for chlamydia trachomatis screening in hospital emergency department settings
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/71e671c7a77547ab9c88c20675696df8
work_keys_str_mv	AT djshin mobilenucleicacidamplificationtestingmobinaatforchlamydiatrachomatisscreeninginhospitalemergencydepartmentsettings AT pathamanolap mobilenucleicacidamplificationtestingmobinaatforchlamydiatrachomatisscreeninginhospitalemergencydepartmentsettings AT lchen mobilenucleicacidamplificationtestingmobinaatforchlamydiatrachomatisscreeninginhospitalemergencydepartmentsettings AT jhardick mobilenucleicacidamplificationtestingmobinaatforchlamydiatrachomatisscreeninginhospitalemergencydepartmentsettings AT mlewis mobilenucleicacidamplificationtestingmobinaatforchlamydiatrachomatisscreeninginhospitalemergencydepartmentsettings AT yhhsieh mobilenucleicacidamplificationtestingmobinaatforchlamydiatrachomatisscreeninginhospitalemergencydepartmentsettings AT rerothman mobilenucleicacidamplificationtestingmobinaatforchlamydiatrachomatisscreeninginhospitalemergencydepartmentsettings AT cagaydos mobilenucleicacidamplificationtestingmobinaatforchlamydiatrachomatisscreeninginhospitalemergencydepartmentsettings AT thwang mobilenucleicacidamplificationtestingmobinaatforchlamydiatrachomatisscreeninginhospitalemergencydepartmentsettings
_version_	1718394991524446208

Mobile nucleic acid amplification testing (mobiNAAT) for Chlamydia trachomatis screening in hospital emergency department settings

Ejemplares similares